We narrowed to 253 results for: IRES

1. Hot Plasmids - February 2022

   Type

   Blog Post

   Published

   Feb. 22, 2022, 2:15 p.m.

   ...label your favorite protein! While the target only requires a small epitope tag (FLAG is eight amino acids...

2. Antibodies 101: Isotypes

   Type

   Blog Post

   Published

   Oct. 19, 2021, 1:15 p.m.

   ...deleted.     Figure 2: Isotype switching requires DNA recombination of the antibody gene locus. ...

3. From Lab Bench to Leadership: Transitioning into Management as a PhD

   Type

   Blog Post

   Published

   Jan. 21, 2025, 2:15 p.m.

   ... goals, and fostering team success. This shift requires deliberate preparation and a willingness to embrace...

4. Plasmids 101: Inducible Promoters

   Type

   Blog Post

   Published

   Jan. 18, 2018, 2:34 p.m.

   ...negative inducible pLac promoter. This promoter requires removal of the lac repressor (lacI protein) for...

5. Luminescent Imaging with Nano-lanterns

   Type

   Blog Post

   Published

   May 25, 2017, 2:30 p.m.

   ...technology. Multicolor imaging using Nano-lanterns requires a linear unmixing algorithm to separate the various...

6. Screening for Successful Genome Editing with Digital PCR

   Type

   Blog Post

   Published

   March 30, 2017, 2:30 p.m.

   ...digital PCR is a great screening tool, it still requires target DNA sequencing to confirm the exact nature...

7. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

   Type

   Blog Post

   Published

   June 25, 2019, 12:54 p.m.

   ...introduced directly into the cell. Microinjection requires the use of a fine needle to deliver nucleic acids...

8. 15 Years of Addgene: The Top 15 Plasmids

   Type

   Blog Post

   Published

   Jan. 8, 2019, 1:54 p.m.

   ...lab that contains the gag and pol genes. It also requires pRSV-Rev and the envelope expressing plasmid pMD2...

9. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

   Type

   Blog Post

   Published

   Dec. 6, 2018, 1:34 p.m.

   ... tagging proximal proteins within minutes, but requires H2O2, which can be toxic in living cells. BioID...

10. A Deep Dive into BioBuilder

    Type

    Blog Post

    Published

    Oct. 11, 2017, 1:44 p.m.

    ...being asked in MIT’s research labs, BioBuilder inspires students and teachers to apply engineering principles...

11. How Addgene Creates Content

    Type

    Blog Post

    Published

    Nov. 17, 2022, 2:15 p.m.

    ... made most of the Antibody Guide figures. This requires a fair amount of communication between our content...

12. Plasmids 101: Methylation and Restriction Enzymes

    Type

    Blog Post

    Published

    June 30, 2016, 2:30 p.m.

    ...overlapping methylation Not affected Not affected DpnI Requires methylation for activity Not affected Not affected...

13. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type

    Blog Post

    Published

    Feb. 10, 2016, 3:30 p.m.

    ...in the 10-12 kb range. REPLACR-mutagenesis also requires making your own electrocompetent cells in which...

14. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type

    Blog Post

    Published

    June 2, 2015, 6:06 p.m.

    ...Although this method shortens construct size, it requires coexpression of Csy4 with Cas9 and the polycistronic...

15. Plasmids 101: Yeast Vectors

    Type

    Blog Post

    Published

    Feb. 25, 2014, 7:11 p.m.

    ... across many different cell types and each one requires different elements for vector propagation. This...

16. Plasmids 101: The protein expression toolbox

    Type

    Blog Post

    Published

    Feb. 16, 2023, 7:05 p.m.

    ...control all the time If your experimental system requires tunable control - or maybe you’re just a control...

17. Visualizing Protein Turnover In Situ

    Type

    Blog Post

    Published

    Jan. 16, 2018, 3:20 p.m.

    ... of radioactive materials is highly regulated, requires designated work areas and come with health risks...

18. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type

    Blog Post

    Published

    Feb. 28, 2019, 1:34 p.m.

    ...thinking about reproducibility and shared similar desires to take action. Thanks to Teytelman’s vast network...

19. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type

    Blog Post

    Published

    Sept. 4, 2018, 12:53 p.m.

    ... example, the synthesis of deoxychromoviridans requires three genes whose proteins are needed sequentially...

20. Developing Lab Management Software for Biology

    Type

    Blog Post

    Published

    Aug. 25, 2016, 2:30 p.m.

    ... itself. If something happens to the worm that requires a change of tube, there’s no clean way to separate...