Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 615 results for: LIS

Showing: 141 - 160 of 615 results

  1. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    Published
    Aug. 21, 2014, 3:15 p.m.
    ...she would be looking at where I had published, who I had published with and whether I would be able to... set up an ELISA kit company on whose website you can find some great information on ELISA troubleshooting...Undergraduate sections and brought in more focus on the list of conferences I presented at and placed them under...troubleshooting, ELISA kits and cell death assays. You can find Seán on twitter @Macfhearraigh.    Read more posts...

  2. Advice for Scientists Starting a Lab

    Type
    Blog Post
    Published
    April 29, 2014, 1:43 p.m.
    ...and foster an effective working environment? Tom Ellis was offered a start-up package that included a postdoc... most surprising thing about becoming a PI? Tom Ellis has a one word answer: “Email. Nothing prepared ...differently or the same as your adviser did? Tom Ellis merges the influence from his two PhD advisers who...who were “chalk-and-cheese”. While one was an established Professor who was always “off flying his plane...our latest results as soon as the gels ran.” Dr. Ellis tries to lead his team as a scientists and a friend... lab!”   Acknowledgments Thank you to Dr. Tom Ellis (Imperial College London), Dr. Connie Cepko (Harvard...

  3. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

    Type
    Blog Post
    Published
    June 6, 2019, 12:41 p.m.
    ...recent plasmid deposit please sign up here.      Listen to this episode of the Hot Plasmids podcast Light-mediated...transmission Article contributed by Klaus Wanisch  Listen to the optogenetics podcast segment An optogenetic...and joined each fragment with one part of an established pair of proteins (iLID and SspB) that undergo...mammalian hosts Article contributed by Lukas Morgan  Listen to the acoustic reporter gene podcast segment In...regulation Article contributed by Michael Lemieux  Listen to the miRNA reporter podcast segment Discovered... CHIME Article contributed by Aliyah Weinstein  Listen to the CRISPR podcast segment Understanding the...

  4. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    Published
    June 29, 2017, 1:06 p.m.
    ... their precise positions within the plasmid. The list of enzymes that can be detected is 5x greater than...GenBank File or a SnapGene file for the sequences listed. You can then analyze the sequence in your sequence...Sequence” tabs. Features The table found here lists the common features detected in the plasmid sequence... map, direction (if relevant), and types. Types listed are standard GenBank feature types. Clicking on...Sequence” tabs. Primers This tab contains a table listing commonly used primers detected in a given nucleotide...Analyzer guide A quick guide to all of the tabs listed above and their functions. We hope that these...

  5. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type
    Blog Post
    Published
    Jan. 30, 2020, 2:15 p.m.
    ...pre-clinical life science work, economics) that published research is difficult to replicate.  Why is replicating...appreciate how few details are provided in most published papers. The precise timing, order, or every combination... rarely exist or are preserved as part of the publishing process and become lost if not archived. Remove... article is given “in-principle acceptance” to publish the final results regardless of the outcome of ...null findings that are notoriously difficult to publish.  With Registered Reports, the authors are able...scientific research. These include policies for publishers and funders in the Transparency and Openness ...

  6. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type
    Blog Post
    Published
    March 19, 2019, 1:31 p.m.
    ... plasmid deposit please sign up here.          Listen to this episode of Hot Plasmids! Optogenetic control...by CASANOVA Article contributed by Cary Valley  Listen to the anti-CRISPR podcast segment Anti-CRISPR ...using blue light (Bubeck et al., 2018). They accomplished this by inserting a photo-sensitive LOV2 domain... Archon Article contributed by Shreya Vedantam  Listen to the voltage indicator podcast segment The neurons... system Article contributed by Michelle Cronin  Listen to the 2in1 cloning podcast segment Recently, the...and AsLOV2 Article contributed by Angela Abitua  Listen to the photoswitchable protein podcast segment ...

  7. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ... Map & Network (CeNGEN) The CeNGEN goal is to establish a comprehensive gene expression atlas of every...publication for the worm community where one can publish brief novel findings. These results are normally...results. This a great resource for scientists to publish findings that don't fit into larger publications... that can be referenced. In addition the data published in microPublications is curated and added to other...but there are Drosophila and zebrafish articles published there as well.   Worm Development Dynamics Database...used for introducing point mutations that was published in a microPublication (Schwartz and Jorgensen,...

  8. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    Published
    Sept. 17, 2015, 2:30 p.m.
    ... impossible to interpret. Polishing the fiber optics with diamond polishing paper of sequentially increasing... second key point; the fiber optics need to be polished to significant smoothness to prevent drastic loss...its output with a light meter before and after polishing. Efficiency of the implant should be at least ... output. The same considerations apply to the polishing and preparation of the patch cable (although you...it’s best to try those that have already been published first. Because behavioral optogenetics is performed...

  9. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ...intellectual property, it worked away in isolation. It published a paper a year (or less) and limited the exchange...public and to other academic labs. Being unable to publish or attend conferences makes it very challenging...As a Master’s student, she had less pressure to publish and more freedom to attend conferences, but after...occasionally thinking about how we could bypass the establishment hiring system altogether. We had a lot of ideas...restricted to science jobs. Keira’s an optimist, I’m a realist, and together we make a pretty good team. We have... – although we didn’t figure out how we would accomplish the color change until a few months later. RevBio...

  10. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ...access to a list of posters until the day-of, take a moment to step aside to read through the list at the ... a day in advance. Dress for the venue and professionalism of the conference, and remember to wear comfortable...about the relationship of your work to recently published papers or “hot topics,” so be sure to be well-...strategically use your time. Don’t have access to a list of posters at all? No problem! At the beginning ...traverse the sea of posters If you come prepared with a list of posters of interest, then you’ve already set ...

  11. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ... can be helpful. Publishing: As a postdoc at AstraZeneca, you are expected to publish, but this can vary...prerequisite “industry experience” that I’ve seen listed as a requirement on some industry job postings....vary depending on which company you choose. If publishing is important to you, make sure that your project... Regeneron, and Genentech, among others, and the list is growing. Some post positions as they come up,... Novartis, have specific web sites for postdoc listings, so the method of posting varies between companies...

  12. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    Published
    Jan. 10, 2017, 3:37 p.m.
    ... thanks to our guest bloggers Alissa Lance-Byrne and Alex Chavez! Alissa Lance-Byrne is a graduate student...This post was contributed by guest bloggers Alissa Lance-Byrne and Alex Chavez, researchers at the Wyss...gene or introduce a desired genetic alteration. Listen to Our Podcast Interview with Alex Chavez ... induce Cas9 to make edits (AC, WLC, and JQ, unpublished results). An additional benefit of the use of...PMC4265475. Additional Resources on the Addgene Blog Listen to Our Interview with Alex Chavez Learn about Sequencing...

  13. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    Published
    Dec. 20, 2017, 2:33 p.m.
    ... new accomplishments to add. Obtain copies and translations of documents that are not in English. Create...development, consulting, policy, big data and the list goes on and on. As you think about your career (... to obtain permanent residence. The recently established case Matter of Dhanasar provides the three-prong...qualify, you need to satisfy 3 out of the 10 criteria listed below. An international or national award for ...therapeutics to cure the cancer. Authorship:  Publishing books, journals online and/or in print. Having...demonstrate outstanding achievement Evidence of published material in professional publications written ...

  14. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ... already answered this question and used it to publish!  Working with AAV vectors in neuroscience Let...performance in our system and yet none of that data was published.    Fig. 1: Testing of three different ... for your system or to successfully replicate published works. Of particular importance to these efforts...build on others’ results is available not through published manuscripts, but through word of mouth or old ...asked your team members or colleagues: How much unpublished AAV data do you have sitting in computers and... O’Connor, D. M., Lutomski, C., Jarrold, M. F., Boulis, N. M., & Donsante, A. (2019). Lot-to-Lot Variation...

  15. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ...made a table that listed each protein in the class, then for each protein we listed all the studies that...include every piece of information that was ever published. Obviously this isn’t possible. So, define your...people cared about. I also used reviews to get a list of research papers that I needed to read. Once I... page as possible. This could be in the form of lists, streams of consciousness, or anything else. Like.... Curate and present some useful data Typically lists and pictures are the most useful parts of reviews...

  16. An Interview with Connie Cepko - Gene Therapy, Plasmid Tools and Insights to Success

    Type
    Blog Post
    Published
    Dec. 8, 2015, 3:30 p.m.
    ...specific subjects in the interview. Happy Listening! Click here to listen! Podcast breakdown: (Time : Topic) ...Harvard Medical School researcher Connie Cepko. Listen to the podcast below to hear all about the research... Edward B. Page is a Digital Media Production Specialist at Addgene. He is the audio engineer and producer... Plasmid Tools Being Developed in the Cepko Lab Listen to our Interview with Michael Koeris Get Cepko ...

  17. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...endogenous homologous recombination enzymes can also accomplish the desired genomic modification. This technique... which results in a merodiploid. This can be accomplished by selecting for an antibiotic resistance marker...counter-selection system relies on the sacB gene product of B. subtilis (Hmelo et al., 2015). The sacB gene product is...binding site (using the online calculator from the Salis group) (Espah Borujeni et al., 2014). Replaced the...scarless modification of bacterial genomes. It can accomplish large deletions, insertions, and even precise..., Amin, Anirudh S. Channarasappa, and Howard M. Salis. "Translation rate is controlled by coupled trade-offs...

  18. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ...get people to remember you). What better way to accomplish all of these things naturally than to give a ... talk without looking at their slides or notes. Listen to their stories and narratives (see below “Tell...presentation appropriately.  Find out who will be listening.  Are they early, mid, or late career?  Will many... Tell a story We are biologically programmed to listen and remember better when we hear material delivered...should be very simple. The goal is to get the listeners to be thinking “What happens next?”  Describe .... During your practice rounds, encourage your listeners to ask questions and record those that were asked...

  19. Stepping into a Virtual Internship: My Experience at Addgene

    Type
    Blog Post
    Published
    Aug. 13, 2020, 1:21 p.m.
    ... Video Production Specialist and Arthur Guerra who is a Customer Support Specialist. Both of these Addgenies...Hola! Que tal? My name is Brandon Solis, and I am a rising senior studying Sociology and Dance at Skidmore...of challenging what I am used to. Brandon Solis, marketing intern. Something that I struggle ... Brandon Solis...

  20. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    Published
    Dec. 19, 2013, 3:46 p.m.
    ...which he submitted a phony paper to a list of 304 open access publishers. By the time the magazine went to...world. 4)      The Annals of Internal Medicine published systematic reviews on an agent used to promote... into account. 7)     Science’s John Bohannon published an article describing a sting operation in which... publications, although many don’t necessarily publish in OA journals themselves. A good number of those...
Showing: 141 - 160 of 615 results