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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 461 results for: MEA;

Showing: 141 - 160 of 461 results

  2. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    Published
    Oct. 9, 2014, 3 p.m.
    ...combinations that can be imaged together, but this also means that the choice of fluorescent proteins requires...this filter set and have reported brightness measurements. Here, EGFP outperforms the improved folding...

  3. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...plasmid components, it may be useful to describe the means of introducing genetic material (such as plasmids... post, we will define positive selection as the means of picking up positive traits (i.e. the plasmid ...

  7. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...generating mutant libraries exist, but none provide a means to continuously diversify all nucleotides within...size at the target site. The use of such libraries means that the diversity introduced by lambda red recombineering...

  8. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ...generally have moderate to high immunogenicity, meaning they induce an immune response in the host. So...endogenous DNA. AAV also has low immunogenicity, meaning it does not induce the same high immune response...

  9. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    Published
    Nov. 10, 2022, 2:15 p.m.
    ...question? If you’re thinking, ‘wait, those terms mean different things?!’, you aren’t alone! We’ve noticed...individually administered and then outcomes are measured. While the entire genome can be knocked out with...

  10. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    Published
    Aug. 19, 2025, 1:15 p.m.
    ... yellow GECIs, OCaMP shows: Higher ΔF/F0 (the measure of signal change during calcium events), Greater...curves for OCaMP and various red and yellow GECIs measured with soluble protein. Reproduced from Aggarwal...

  12. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    Published
    Jan. 28, 2020, 2:35 p.m.
    ... for milk processing, fruit juice clarification, meat tendering, and oligosaccharide synthesis. A major...function well under suboptimal conditions. This means recombinant proteins can aggregate or precipitate...

  13. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    Published
    May 22, 2014, 4:02 p.m.
    ...conventional journal. Publishing via open access means that your manuscript will be available to anyone... for publication and is, in most cases, blind (meaning you don’t know who the reviewers are). If the reviewers...

  14. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ...yeast-specific origin of replication (ORI) and a means of selection in yeast cells, in addition to the ...some drawbacks to using auxotrophic markers as a means of selection: A specific selection marker needs...

  15. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    Published
    June 15, 2017, 2:30 p.m.
    ...There are a variety of methods that can be used to measure your fluorescent protein’s propensity to aggregate...an affinity of 110 µM (Zacharias et al, 2002), meaning that at this concentration 50% of the protein forms...

  16. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    Published
    Jan. 11, 2017, 3:30 p.m.
    ...ahead with raises. What will these further changes mean? The abrupt reversal in salary raises at institutions... La Jolla California are not getting a raise. Meanwhile, in less densely populated places like the University...

  17. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...designing a website users could easily navigate. This meant we could grow accessibility alongside our repository...beginners” on r/labrats, Reddit.  Part of that work meant facilitating the Material Transfer Agreement process...

  18. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ... used in research have their genomes “gutted”, meaning that all viral genes except ITRs (inverted terminal...capacity Empty rAAV has a cargo capacity of ~ 4.7 kb, meaning that your gene of interest (or genes) need to be...

  19. The time and cost required to make a plasmid

    Type
    Blog Post
    Published
    March 23, 2023, 1:15 p.m.
    ...put scientific inflation at… roughly 34.9%, which means that the same plasmid would cost $155.25 in reagents...changes. Fun fact: Addgene’s high-throughput approach means we virtually always use every single well in a 96...

  20. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...protein of interest. The tag was developed as a means to purify a protein based on the affinity of the...losing your protein of interest. But that doesn’t mean longer is always better - the longer the tag, the...
Showing: 141 - 160 of 461 results