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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 169 results for: PHI-1;

Showing: 141 - 160 of 169 results

  1. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ... difficult to modify in the past.   Figure 1: Identification and enrichment use of XMAS-TREE. a...DNA of interest, and cell extract.    Graphical Abstract: Schematic representation of cell-free...

  2. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    Published
    May 6, 2014, 1:07 p.m.
    ...variants. This resulted in the construction of redCALWY-1, a red-shifted FRET sensor for zinc using variants... his calcium indicators can be used in mouse, Drosophila, C. elegans, and zebrafish – essentially all ...

  4. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ... cell types and physiological states. References 1. Ingolia, Nicholas T., et al. “Genome-wide analysis...Halstead et al. used TRICK to examine translation of Drosophila patterning gene oskar. Early in development, ...

  5. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    Published
    Jan. 12, 2021, 2:15 p.m.
    ...defunct (but fun) plug-in called Maps.  Figure 1: See a starfish? This is actually a map of Joanne ...Networking connections can last years This is a graphic snapshot of my LinkedIn network from a few years...

  6. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    ...vector-exosomes), exosome AAVs, or exo-AAVs (Figure 1). The researchers discovered that these particles ...coagulation factor IX in liver in a mouse model of Haemophilia B (Meliani et al., 2017). Exo-AAV9 was successfully...

  7. Components of CRISPR/Cas9

    Type
    Blog Post
    Published
    Feb. 2, 2016, 5 p.m.
    ...individual protein has all the components necessary to: 1. Bind to a guide RNA The guide RNA, which will be ...meningitidis (NM) 3' NNNNGATT  Streptococcus thermophilus (ST) 3' NNAGAAW  Treponema denticola (TD...

  8. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    Published
    June 4, 2015, 1:03 p.m.
    ...and also by a lactose analogue, IPTG (isopropyl β-D-1-thiogalactopyranoside). (To be completely accurate...reproduce to form colonies (used to complement auxotrophic cell lines), or genes which rescue a lethal phenotype...

  9. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    Published
    Nov. 24, 2020, 2:15 p.m.
    ... UK team members before they log off for the day. 1:45 PM--Write final report. Work on a final report ...what the day to day looks like. Check out the infographic for an outline of my day or find the text version...

  10. Antibodies 101: Isotypes

    Type
    Blog Post
    Published
    Oct. 19, 2021, 1:15 p.m.
    ...region: IgM, IgD, IgG, IgE, and IgA.    Figure 1: Diagram of an antibody labeled with Fc, Fab, heavy...found bound to IgE receptors on the surface of basophils and mast cells, two types of white blood cells...

  11. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    Published
    Jan. 25, 2024, 2:15 p.m.
    ...and CasPEDIA have got you covered!     Fig. 1 – CasPEDIA Cas ID display information for a given ...to relevant resources The homepage displays a graphic describing CasIDs and relevant properties of Cas...

  15. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    Published
    Nov. 3, 2015, 3:30 p.m.
    ...first of only three such databases, EENdb (published 1 Jan 2013, no longer available), is a simple catalogue...follow him on Twitter @CMacPhD.          References  1. Ghorbal, Mehdi, et al. "Genome editing in the human... not. It is an offline tool, but comes with a graphical user interface. Best strategy for labs: Define...

  16. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...significant progress in clinical trials.     Figure 1: Types of delivery and editing strategies for CRISPR...is caused by a variety of single nucleotide polymorphisms (SNPs) in the TTR gene and is molecularly characterized...

  17. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...viruses - 2 cas systems regulate at the DNA level and 1 at the RNA level. Udi Qimron from Tel Aviv University...invasion.  His lab is studying P. furiosus, a hyperthermophile that exists in sea vents at the boiling point...

  18. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ... – a mechanical device that could be used to bend 1/8th inch steel wire into a backbone structure of a...popularity was short-lived as powerful molecular graphics packages were soon developed that allowed researchers...

  20. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    Published
    Aug. 10, 2021, 1:15 p.m.
    ...target DNA quickly and accurately.  Figure 1: Steps in PCR reaction   This process of denaturation...relies on heat resistant DNA polymerase from the thermophilic bacterium, Thermos aquaticus (Taq). Taq polymerase...
Showing: 141 - 160 of 169 results