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We narrowed to 464 results for: SHI

Showing: 141 - 160 of 464 results

  1. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ...01039-7 Nishimasu, H., Ran, F. A., Hsu, P. D., Konermann, S., Shehata, S. I., Dohmae, N., Ishitani, R., ...disrupting its canonical structure and Cas9 binding (Nishimasu et al., 2014). Figure 3: pegRNAs whose...

  2. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ...Similarly, if you want to knock out a gene, a frameshift mutation introduced anywhere within the early...with NG PAM compatibility, as the name implies (Nishimasu et. al, 2018). xCas9 was generated by the Liu ...10.1126/science.aba8853 (Plasmids available here)   Nishimasu, H., et al.,Engineered CRISPR-Cas9 nuclease with...

  3. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ...all of your plasmid data, we’ll send you prepaid shipping materials (i.e., a deposit kit) with instructions...we need to know your Addgene account username. Shipping address and phone number. Plasmid sequences or...deposit kit with instructions on how to prepare and ship the samples to Addgene and contact your technology...

  4. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ...– $30,000 for research and another $30,000 in mentorship and business training. The money is a drop in...thinking about making the switch from academia to entrepreneurship, share your thoughts with us in the comments...guest bloggers! Nikolai Braun grew up outside of Washington D.C. and has always enjoyed exploring the outdoors...

  5. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ... situation where one wants to understand the relationship between the age of a cell, protein, or cellular...: 1585-1588. PubMed PMID: 11090358. 2. Tsuboi, Takashi, et al. "Age-dependent preferential dense-core ... 118-126. PubMed PMID: 19136976. 5. Miyatsuka, Takeshi, Zhongmei Li, and Michael S. German. "Chronology...

  6. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...environment sooner rather than later can be helpful. Publishing: As a postdoc at AstraZeneca, you are expected...vary depending on which company you choose. If publishing is important to you, make sure that your project...an industry setting. Scientific community and mentorship: As an industry postdoc, you might find yourself...

  7. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...construct targeting vectors. Addgene depositor Takashi Yamamoto’s lab has harnessed MMEJ to create a new...NHEJ joins the ends of a DSB in an error-prone fashion, with insertions and deletions common. In contrast...possibilities MMEJ can enable. References 1. Sakuma, Tatsushi, et al. (2016).  “MMEJ-assisted gene knock-in ...

  8. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ...and Challenges CUREs inspire students to take ownership of their work and become more actively involved...Biology Department at Keene State College in New Hampshire. He mentors undergraduates conducting NIH- and...):ar55. Ma, Y., Mukherjee, S., Uzzi, B. 2020. Mentorship and protégé success in STEM fields. Proceedings...

  9. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...equally effective are shipped to customers on ice, preventing any temperature shifts. Of course, we are ...Wang et. al., 2018). Aggregation can arise from a shift in temperature, pH, or salt concentration (Amin ...

  10. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

    Type
    Blog Post
    Published
    July 1, 2025, 1:15 p.m.
    ...A260/230 ratios. Instead, phenol is more likely to shift the peak of your sample's absorption spectra from... RNA and DNA absorb maximally at 260 nm, so distinguishing between the two can be difficult by spectrophotometry...higher A260/280 and a lower A260/230 than DNA, so shifts in these values could hint at the presence of RNA...

  11. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ...employees. The teambuilding culture helps us build relationships and thereby work better together.I realize I...encourage everyone to make an effort to build relationships with their colleagues. It can be easy to get...sometimes fun needs to be had in an orderly and timely fashion. 9. Ask for help My grandma used the old adage...

  13. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ...Sadowski 1985). The FLP system works in a similar fashion to the Cre system. The FLP recombinase recognizes...inversion, and translocation of DNA in a similar fashion as between loxP sites (For details see Cre-lox ...the human brain.     Acknowledgement:Through a partnership with genOway, we are able to distribute materials...

  14. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ... the service has grown steadily, helped by a partnership with the University of Pennsylvania’s Vector ...2023. And while viral vectors are not as easy to ship as plasmids, they still have a global reach. From...this drove Addgene to develop new systems, new partnerships, and new platforms, from our early engagement...

  15. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ...as we obtain more genomic data about plasmids (Shintani et al., 2015). Now, bioinformatics tools help ...10.1016/j.sbi.2011.11.001 Pubmed PMID: 22153351 Shintani M, Sanchez ZK, Kimbara K (2015) Genomics of microbial....2015.00242 Pubmed PMID: 25873913 Uraji M, Suzuki K, Yoshida K (2002) A novel plasmid curing method using incompatibility...

  16. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    Published
    Feb. 18, 2020, 2:15 p.m.
    ...structure with regard to the Mentor-Singleton relationship. Some of the variability was organic, as some...coffee breaks. While this was not the more intense mentorship experience that happened at ASM Microbe, we connected...Science’s (SACNAS) annual conference all foster mentorship in different but successful ways. Scientific ...

  17. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ...could have a shipping sprint and all the stories will touch different parts of the shipping code. The following...that the checkout takes the user directly to the shipping step, and no longer takes the user to a terms ...

  18. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    Published
    Oct. 25, 2016, 2:30 p.m.
    ...come under this ruling, regardless of visa or fellowship status, in addition to certain staff scientists...Department of Labor (for example here and here) pushing for exemptions for postdocs, and lowering of the...set up other institutions (like University of Washington) but as discussed elsewhere, there seems no basis...

  19. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ...activities depending on the expertise of the senior leadership and members of their consulting staff. Who would... tasks, and then executing on them in a timely fashion while allowing for creative solutions to emerge...performance, and occasionally being faced with “shifting goal posts” as a part of a case, consulting can...

  20. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ...light of the policy, hold onto data longer before publishing. But there are strong incentives to publish such...data initiatives are also taking root. Nature Publishing Group recently launched a publication called ...veracity of collections and interpretation of data, publishing complete data, rather than just a single projection...
Showing: 141 - 160 of 464 results