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We narrowed to 575 results for: SIM

Showing: 141 - 160 of 575 results

  1. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    Published
    Oct. 8, 2024, 1:15 p.m.
    ...by daughter cells during cellular reproduction, similar to the host’s chromosome, or they can spread between...plasmids likely have undiscovered functions, many are simply genetic parasites. Cryptic plasmids tend to be ... R., Sailland, A., Auriol, P., Vogel, T. M., & Simonet, P. (2001). Laboratory-scale evidence for lightning-mediated...

  2. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ... components. Using the handy website, users can simply select the components they want for each module...license.   Once you have the components you want, simply assemble them into a destination vector via Golden...summary on the Fragmid website.   Step 3 is simply a review of the assembled plasmid. It shows you...

  3. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ...secondary structure to induce transcript cleavage, similar to the Rho-independent termination found in prokaryotes...terminate transcription. Polymerase I uses a process similar to the prokaryotic Rho-dependent mechanism, whereas...Definition of the upstream efficiency element of the simian virus 40 late polyadenylation signal by using in...

  4. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    Published
    Jan. 10, 2017, 3:37 p.m.
    ...bioengineering, as it has facilitated the development of a simple and scalable means of making targeted genetic edits...for instance, the challenge of attempting to simultaneously and selectively induce both cutting and regulation... targeting a different set of loci to induce simultaneous cutting and transcriptional activation or repression...

  5. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    Published
    July 2, 2020, 12:40 p.m.
    ...biotinylated by BirA. From here, the CAPTURE method is similar to other 3C methods: crosslinking captures chromatin...well-characterized beta globin locus. The two methods had similar rates of specificity for the DNA sequence targeted...multiplexing and high resolution allowed the Xu Lab to simultaneously characterize the ‘social networks’ of 157 super...

  6. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ...perform its helicase and nuclease functions simultaneously, by continuously unraveling dsDNA, pulling ...reeling of ssDNA and unwinding of dsDNA occur simultaneously, which creates a loop in the target strand ...is not as well understood but likely occurs in similar regions. This combination of chewing up one stand...

  7. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ... Where publication is expected, the process is similar to academia. The only difference is that your paper...postdocs and do not support or manage them well are simply no longer allowed to have postdocs. In academia...could be laid off while you’re there. Of course, similarly, an academic advisor could also lose their funding...

  9. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    Published
    Sept. 6, 2016, 2:30 p.m.
    ...the accelerator of a vehicle being stuck down. Similarly, a mutation that turns a tumor suppressor “off...cell to undergo programmed cell death while simultaneously stimulating growth and division. The combined...pathway. Its major appeal is that it enables the simple construction of vectors to study the function of...

  10. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ...increase transduction efficiency, none allow for simple and efficient transduction of both the CNS and ... not lost in a jumble of fluorescent neurons. A simple but flawed approach to multi-color labeling is ...PubMed PMID: 28671695. 2. Deverman BE, Pravdo PL, Simpson BP, et al. Cre-dependent selection yields AAV variants...

  11. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...constructs robustly, quickly, and simply. We hope that you can similarly apply MXS chaining to your experimental...Well suited for the modular assembly of highly similar or repetitive sequences. Intermediate chaining ...

  12. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...Nanobodies can be labeled with up to 3 dyes, yielding similar signal strength to a standard IgG secondary antibody...two-step staining with antibodies. Nanobodies also simplify multicolor staining experiments because they don...with this multi-color staining workflow yields similar localization patterns as cells stained for one ...

  13. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    Published
    Jan. 11, 2018, 3:29 p.m.
    ...and I guarantee you have developed them through similar experiences in graduate school. These skills will...they use, you’ve gained experience working with similar protocols, and look - they’re seeking someone who...strength until it is identified by another person. Similar reasoning may also apply to other acquired skills...

  14. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ... we will mostly use the gamma-retroviruses as a simple spokesmodel for the whole family; however, you ...polymerase gene may seem like a mystery. But it’s simple. The RT protein is made by a separate set of instructions... a tool is done, and the transgenic genome will simply express from whatever promoters were included in...

  15. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    Published
    Dec. 3, 2019, 11:38 a.m.
    ... filters you do not need to start a new search. Simply click the ‘X’ next to any filter to clear it. ... Tags: Identify a specific marker protein Use: Similar to “Vector Type” you can filter by experimental... plasmids that may be difficult to define for a simple search. These pages provide helpful information...

  16. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type
    Blog Post
    Published
    March 17, 2016, 2:30 p.m.
    ...minigenomes are incapable of producing infectious virus (similar to lentiviral vectors) because they do not encode...components of the Zaire ebola virus minigenome system Similar to other RNA viruses, EBOV brings its own RNA-dependent...studied independently of other viral proteins simplifying the interpretation of results. This system can...

  17. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ...done annually - they are fan favorites! 6. Keep it simple This may be an obvious one, but don’t make things...really! We've had plenty of events that included a simple corn hole set and a cooler. Chances are one of ...company’s intranet. Don’t forget about the power of simple word of mouth - be sure to bring up your event ...

  18. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ...less risk averse. Similar to the elevated plus maze, the light-dark box is a simple device comprised of...downregulating expression of miR124a achieved a similar improvement in the forced swim test.  Another brain...

  19. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    Published
    Aug. 13, 2019, 12:58 p.m.
    ...transgenic zebrafish are well established (Felker and Mosimann, 2016). One common and robust strategy uses a ...tail. Compared to the opaque mouse embryo, where similar processes occur over days inside the mother, the... Central PMCID: PMC6247916. Felker, A., and C. Mosimann. "Contemporary zebrafish transgenesis with Tol2...

  20. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    ...tobacco, Agrobacterium infiltration into leaves is a simple and efficient way to overexpress a gene and evaluate...abaxial site (lower surface) of the leaf. This is a simple and easy technique to implement. Success might ... Li, Jian-Feng, et al. "The FAST technique: a simplified Agrobacterium-based transformation method for...
Showing: 141 - 160 of 575 results