We narrowed to 186 results for: SPL;

1. Popular Retroviral Vectors and Their Uses in Scientific Research

   Type

   Blog Post

   Published

   July 24, 2018, 12:51 p.m.

   ...purposes, retroviral vectors have been developed into ‘split-vector systems’ by separating viral genes and transgene...

2. Adeno Associated Virus (AAV) for Cell and Gene Therapy

   Type

   Blog Post

   Published

   Nov. 7, 2017, 1:59 p.m.

   ...direct evolution, capsid shuffling, and peptide displays. These capsids will be selected both for their...

3. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

   Type

   Blog Post

   Published

   Sept. 26, 2019, 12:50 p.m.

   ...no detectable editing. Additionally, off-target spleen editing was not detected and Nanoblade injection...

4. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

   Type

   Blog Post

   Published

   April 6, 2021, 12:30 p.m.

   ...chicken beta actin promoter, and rabbit beta-globin splice acceptor, contains sequence that is over 80% GC...

5. Viral Vectors 101: Virus Safety

   Type

   Blog Post

   Published

   June 22, 2023, 1:15 p.m.

   ...or face shields are required whenever there’s a splash or spray risk in BSL-1. In BSL-2 labs, eye protection...

6. Progress Towards a PAM-Free CRISPR

   Type

   Blog Post

   Published

   March 7, 2024, 2:39 p.m.

   ... reliability. Notably, the SpRY variant didn’t display higher off-target effects than SpCas9, a pleasant...

7. Cpf1 Update: Comparison to Cas9 and NgAgo

   Type

   Blog Post

   Published

   July 14, 2016, 2:30 p.m.

   ... cleavage. Now, two new studies show that Cpf1 displays lower off-target editing than Cas9, confirming...

8. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

   Type

   Blog Post

   Published

   Feb. 7, 2019, 2:16 p.m.

   ...happens often because the HDR inserted sequence splits up the guide sequence.) If neither of the above...

9. Cloning Mammalian Cells with the Agarose Method

   Type

   Blog Post

   Published

   Sept. 7, 2017, 12:17 p.m.

   ...efficiency is not known, this is easily achieved by splitting transfected cells into 3 plates at different densities...

10. Magnetic Control of Proteins: More than a Dream

    Type

    Blog Post

    Published

    Sept. 24, 2024, 1:15 p.m.

    ...) and to see if any other fluorescent proteins display a similar effect (many do). Fusing EGFP to the ...

11. Of Myc and Men

    Type

    Blog Post

    Published

    Jan. 19, 2023, 2:15 p.m.

    ... E (2020) The Myc tag monoclonal antibody 9E10 displays highly variable epitope recognition dependent ...

12. PITChing MMEJ as an Alternative Route for Gene Editing

    Type

    Blog Post

    Published

    Feb. 23, 2016, 3:30 p.m.

    ...clones, they found that 80% and 50% of clones displayed proper insertion at the 5’ and 3’ junctions, respectively...

13. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type

    Blog Post

    Published

    Dec. 3, 2019, 11:38 a.m.

    ...the number of plasmids matching each filter is displayed in parentheses.  In this example I wanted to find...

14. Summer SciComm Series: Modes of Communication

    Type

    Blog Post

    Published

    July 1, 2022, 1:15 p.m.

    ...  No visuals. Variety of hosting platforms can splinter audience. Primarily digital distribution (though...

15. Lentiviral Vector Uses and Overview

    Type

    Blog Post

    Published

    May 19, 2016, 2:30 p.m.

    ...in two key ways. First, the packaging system is split into two plasmids: one encoding Rev and one encoding...

16. Transferable Skills Guide: External Collaborations

    Type

    Blog Post

    Published

    April 19, 2018, 1:25 p.m.

    ...partnership with SnapGene, for example, allows us to display high-quality maps and annotations without us having...

17. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

    Type

    Blog Post

    Published

    Dec. 14, 2017, 2:08 p.m.

    ...reprogrammed immediately after collection [2]. PBMCs display unique epigenetic signatures that are closer to...

18. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type

    Blog Post

    Published

    Nov. 19, 2020, 2:15 p.m.

    ...editing, MECP2 reactivation, RNA editing, RNA trans-splicing, and protein replacement to reverse the impacts...

19. Plasmids 101: Plasmid Incompatibility

    Type

    Blog Post

    Published

    March 31, 2020, 1:15 p.m.

    ...plasmids. This strategy has been successful in displacing, or “curing” the virulence plasmids from the ...

20. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type

    Blog Post

    Published

    Aug. 13, 2019, 12:58 p.m.

    ...its translation either by blocking initiation or splicing. (RNAi, in contrast, engages existing gene regulatory...