...Fluorophores In this post, Luke Lavis from Janelia Research Campus reminds us that, although fluorescent proteins.... To learn how you can use minigenomes in your research, check out this post! R Bodies: Membrane-Rupturing... post, Jessica Polka, formerly a postdoctoral researcher in the Silver Lab at Harvard University, describes...CombiGEM is a technology that can be used to quickly barcode a set of DNA sequences. In this post, Alan Wong... Lab at MIT) shows how you can use CombiGEM to barcode small libraries of CRISPR gRNAs to use for screening...Genome Editing CRISPR has revolutionized how researchers make knockouts and other types of genome edits...In this post, Chris Richardson, a postdoctoral researcher in Jacob Corn’s lab, describes how you can better...

...10.1016/j.cell.2020.12.015 Garcia-Beltran WF, Lam EC, St. Denis K, Nitido AD, Garcia ZH, Hauser BM, Feldman...current vaccines differed depending on the variants (Garcia-Beltran et al., 2021). Antibodies from individuals...the P.1 and B.1.351 variants tested. Image from Garcia-Beltran et al., 2021 with permission.   The...quantified in terms of luminescence. Image from Garcia-Beltran et al., 2021 with permission.   Mutations...sera with various pseudotyped virus. Image from Garcia-Beltran et al., 2021 with permission.   Difference...plasmids at Addgene to accelerate ongoing COVID-19 research. “I hope that by sharing the plasmids other scientists...146. https://doi.org/10.1038/s41586-021-03471-w Garcia-Beltran WF, Lam EC, Astudillo MG, Yang D, Miller...

...agreement that states the terms of use for a tangible research material that is shared between two parties. There... for organizations to make policy decisions on research use agreements. The UBMTA, which is the MTA most...well-understood and accepted by the majority of research institutes.  Pro tip! In most academic and nonprofit...Transfer department is responsible for managing research use agreements like MTAs.  Addgene does a lot ...plasmids at Addgene are shared under the UBMTA, researchers additionally have the option of distributing ...which allows the plasmids to be distributed for research use only to industry-based scientists. Some organizations...OpenMTA, which allows plasmids to be used for both research and commercial purposes. It’s important to note...

...database is to browse or search information in more powerful ways, and the advanced search page simplifies the...contributed by guest blogger Talley Lambert, a Research Associate at Harvard Medical School. The need ... most indispensable tools in modern biological research.  Any microscopist will tell you that selection...has been very useful over the years, and when researchers come to us for advice on choosing a protein, ...particular FP; this can be done directly using the search bar on the homepage (and at the top of all pages...the process of building advanced multi-parametric search queries.  Looking for a bright, monomeric FP published...guest blogger Talley Lambert! Talley Lambert is a Research Associate at Harvard Medical School. He manages...

... results helps other researchers using these plasmids and will give your research more visibility.    ...By Will Arnold and Shreya Vedantam Accelerating research by improving the availability of materials is ...to help academic and industrial labs accelerate research and to help find a faster solution to the COVID...coli and S. cerevisiae expression vectors in late March. The first set of 383 constructs went online just...samples, the rest will be put into a status we call “Archived.” This status allows us to store plasmids and ... is indeed global. Helping scientists speed up research is what we’re here for and we’re glad to be able...Ginkgo Bioworks  Find COVID-19 plasmids for your research Browse the Ginkgo Bioworks COVID-19 Plasmid Collection...

...A 2017 survey found that many researchers feel they lack formal training in a variety of transferable...out to fill this gap by both highlighting that researchers do learn MANY transferable skills while working... into play while puzzling through your broader research questions and designing future experiments. Being...While I was in graduate school and beginning to research non-academic careers, I always felt that descriptions...when you’re faced with yet another hurdle in your research journey.  What are ‘problem solving skills’? As... about the world. You might do some background research to better understand it and come up with several... of your skill set later on. If you are new to research and the lab, you may not be able to carry out ...

...represents a unique funding model in nuclear receptor research, and has generated a set of comprehensive datasets... questions in nuclear receptor and coregulator research. Our Q-PCR datasets, for example, document the... today.  The sheer abundance of these critical research tools is a double-edged sword, however, and has...burden associated with identifying plasmids in the research literature and acquiring them in a timely manner...to the problem of sharing plasmids, providing researchers with a one-stop shop for plasmids and vectors...vectors, and making them available to all researchers for a reasonable fee. We at NURSA recognize the importance...importance of this service to the NR research community and, accordingly, the new version of the NURSA website...

...off-target cleavage events. Unbiased techniques search the whole genome for off-target sites irrespective... in concert, these approaches can provide the researcher with a reasonable level of certainly that the...sgRNA. This is a good starting point for most researchers as it provides a list of putative off-target ...can later sequence for mutations. One method a researcher can use to test predicted off-target sites following...to perform these validations as translational researchers begin to use CRISPR as a therapy. Low frequency... effects may generate irreproducible data in a research setting, but these events could have disastrous...off-target prediction algorithms, their genome-wide search criteria are not exhaustive. Mismatch tolerance...

...on a stochastic search algorithm rather than pre-designed sets, so identical searches can return multiple...particularly for high-complexity reactions? The researchers over at New England Biolabs® thought otherwise...design (DAD) to build out three tools to help researchers design high-fidelity Golden Gate Assemblies and...three tools developed, outlined below, allow for researchers to select large sets (with overhangs that violate...You can define the number of fragments, and the “search windows” you’d like the tool to use to identify... can exclude specific sequences or set a narrow search window for a site—or sites—that must be used. Each...necessarily require cycling. The Pros and Cons Researchers used the tools above to complete a 52-piece assembly...

... most commonly used research tools in scientists’ pockets. They allow researchers to identify and isolate...an antibody that will be essential to your new research project, but unsure which one will mark your target...bind to the target). Unreliable antibodies cause research that cannot be reproduced and projects that cannot... with collaborators and support from the Brain Research Through Advancing Innovative Neurotechnologies...Dr. Wu please contact us here! Ava Shipman is a research assistant in lab of Zhuhao Wu at the Icahn School...Reproducibility: Standardize antibodies used in research. Nature 518, 27–29 (2015). https://doi.org/10.1038...The Economics of Reproducibility in Preclinical Research. PLoS Biol 13(6): e1002165. https://doi.org/10.1371...

...students work closely with local researchers to learn about their research while creating physical models...graphics packages were soon developed that allowed researchers to create stunning, interactive images of their... only wonder what I am talking about. How do researchers use physical models of protein structure? We ...been creating physical models of proteins for researchers for the past fifteen years. On those occasions...following sequence of events plays itself out: The researcher breaks eye-contact with any other human in the... and to create custom models of proteins for researchers. Modeling CRISPR We were excited when Jacob Corn...strand. And this is why we love working with researchers on a model. The little knowledge that we have...

...plasmid from our search page (check out this video for a walkthrough of the ways you can search our website...trail at the top of any plasmid page gives the hierarchy of web pages the plasmid page is nested in. You...website). A quicker way to find this plasmid through search is... The Addgene plasmid number: This is the unique...know the catalog number of the plasmid you are searching for, this is the quickest and easiest way to find...than 50; and blue, more than 100 times! When you search for plasmids on our website, one of the available...see if the plasmid would be useful for their own research, and helps requestors use the plasmid in their...citations for Addgene plasmids you have used in your research! Sequence information: Depositors may provide ...

...longer than we’ve been studying it and, now, researchers are exploring ways to bolster and modulate our...protein blocking. Other molecular targets under research include TIM-3, LAG-3, STAT-3, EGFR, CD20 and CD73...to activate them are a few methodologies being researched. Apoptotic and necroptotic tumor cells have also...engineering have played a huge role. For instance, researchers are genetically altering tumor cells and immune... treatments enter the clinic, physicians and researchers are learning much about how treatment regimens... made more effective. Some important factors researchers are beginning to focus on include: Cell heterogeneity... important questions in this exciting field of research. One of the most important: How will cancers evolve...

...acids research 21.15 (1993): 3601. PubMed PMID: 8346047. PubMed Central PMCID: PMC331480. 3. García-Nafría... post was contributed by Jake Watson and Javier García-Nafría from the MRC Laboratory of Molecular Biology...cloning protocol In Vivo Assembly (IVA) cloning (Garcia-Nafria et al., 2016) uses the bacterial recombination...thanks to our guest bloggers Jake Watson and Javier García-Nafría from the MRC Laboratory of Molecular Biology...Biology. Jake Watson is a postdoctoral researcher in neurophysiology at the MRC Laboratory of Molecular...synaptic transmission and plasticity.     Javier García-Nafría is an investigator scientist at the MRC ...    Follow them on Twitter @jakefwatson and @JGarciaNafria.  References 1. Jones, Douglas H., and Bruce...

...immunotherapy, but this research is still in the pre-clinical/early clinical stages. Researchers are also working...vector history and components In the early 1990s, researchers developed viral vector systems based on retroviruses...other difficult-to-transfect cells. Lentiviral barcoding libraries have been used for complex applications...response, it should come as no surprise that researchers are interested in developing these vectors for...vectors have become standard in gene therapy. Researchers have also developed integrase-deficient lentiviral...come a long way since the 1990s, both in terms of research impact and safety. Many of Addgene’s most requested...Virus Biosafety Find Lentiviral Vectors for Your Research  ...

... preps for COVID-19 research, pooled CRISPR lentiirus libraries, pooled barcoding libraries, and more.... you’re working with AAV or lentivirus in your research, you might want to use one of our virus backgrounds... virus background.           Maybe your viral research has taken you in the direction of COVID-19. We...find plasmid tools to accelerate your COVID-19 research.  Download the red COVID-19 background.       ...Here, you can browse plasmids by field, organism, research technique, and more. Download the green DNA background...whether you use molecular biology tools in your research or not! And learn more about the variety of careers...

...typically a professor who operates a research group. Within that research group, you will also typically receive... waiting for you, prep for research work has to be done by the researchers themselves.  Be mindful ...more, depending on the mentoring style within the research group). The bench mentor-mentee relationship ...relationship will probably define your experience in the research group more (at least at first) than any other ...Getting the most out of the training experience In research there is a saying that if the sample prep is poor...fulfilling experience; even the grumpiest of researchers can enjoy it at times! What makes it rewarding...

...students and researchers who do take the initiative to develop themselves, resources are often scarce and difficult... A recent survey of PhDs found that many researchers feel that they lack formal training in a variety...out to fill this gap by both highlighting that researchers do learn MANY transferable skills while working... is clearly a good idea to be diligent in your research, remember that you will not be a graduate student...have a well-groomed LinkedIn page. Here you can search for jobs, post your CV, and, most importantly, ... can be critical to a focused and effective job search. As with any online forum, there is an etiquette... someone a lot older than you are; try using ResearchGate to see where other graduate students and post-docs...

...Initiative being to cure cancer, more funding and research opportunities are being provided to immunotherapy...Therefore, mesothelioma doesn’t receive nearly as much research funding from the government as more common cancers...breast cancer (about 246,000 diagnosed this year). Research efforts in mesothelioma instead rely upon private... they are exposed even one time. Doctors and researchers don’t entirely understand why some people develop..., along with other forms of cancer. Additional research is being done to see what, if any, other genetic...immunotherapy, and angiogenesis inhibitors New research is also going into two other areas that could ...than ever. From breath tests to blood tests, researchers are hopeful that they can come up with new ways...

...Biotechnology and Biological Sciences Research Council (BBSRC) and Medical Research Council (MRC) require authentic...the amount of money wasted in basic oncological research due to contamination are in the tens or hundreds...Resistance and Metastatic Potential in Adenoid Cystic Carcinoma, Up-Regulation of pVHL along with Down-Regulation...lines deposited with them and used by different researchers are free of contamination and match their original...perhaps a working cell bank has been made by a research institution (an excellent practice), or maybe ...Warwick University, gaining extensive cell culture research experience through working for a variety of pharmaceutical..., HeLa cells, and cell culture contamination." Archives of pathology & laboratory medicine 133.9 (2009...