We narrowed to 284 results for: cki

1. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

   Type

   Blog Post

   Published

   Aug. 22, 2019, 12:43 p.m.

   ...for specific amino acids. When plated on media lacking the essential amino acid, the bacteria will only...

2. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

   Type

   Blog Post

   Published

   June 11, 2019, 1:16 p.m.

   ...images. For example, by selecting “Tools” and then clicking the “Usage rights” dropdown menu, you can search...

3. Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene

   Type

   Blog Post

   Published

   July 2, 2015, 8:37 p.m.

   ... days talking to researchers about their work, picking up plasmid deposits, and trying to learn ways Addgene...

4. Choosing Your Perfect Empty Backbone

   Type

   Blog Post

   Published

   Aug. 19, 2014, 3:39 p.m.

   ...to be assayed (e.g. pPD95_86 and pPD57_56), or knocking down YGOI through RNA-mediated interference (e.g...

5. Choosing a Good Mentor for Scientists

   Type

   Blog Post

   Published

   Jan. 16, 2014, 2:56 p.m.

   ...advisors learning how to be a scientist. It is shocking how little pre-work most PhD students and postdocs...

6. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

   Type

   Blog Post

   Published

   Sept. 18, 2018, 1:33 p.m.

   ...Quantification of editing efficiencies simply involves tracking the ratio of mCherry-positive to eGFP positive...

7. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

   Type

   Blog Post

   Published

   Dec. 13, 2018, 1:14 p.m.

   ...Clathrin-mediated endocytosis and subsequent endo-lysosomal trafficking of adeno-associated virus/phage." Journal of ...

8. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

   Type

   Blog Post

   Published

   June 2, 2020, 1:15 p.m.

   ...injection of precise titrations of AAV or heat-shocking the organism. The SPARC system uses competitive...

9. Meet the New Addgene Board Members!

   Type

   Blog Post

   Published

   April 28, 2020, 1:14 p.m.

   ...Library, a library that “enables the high-resolution tracking of more than 1 million cancer cells under drug...

10. New and Upcoming Viral Vectors - December 2019

    Type

    Blog Post

    Published

    Dec. 19, 2019, 2:40 p.m.

    ...pAAV-hSyn-DIO-EGFP   Biosensor AAV Calcium sensors Tracking calcium dynamics that govern such activities as...

11. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type

    Blog Post

    Published

    Aug. 18, 2023, 4:15 p.m.

    ...just on the disease front. Viruses survive by hijacking the machinery of the cells they infect. Once they...

12. Antibodies 101: Epitope Tags

    Type

    Blog Post

    Published

    May 11, 2023, 1:15 p.m.

    ...application. Depending on the tag, directly imaging or tracking your protein may also be possible, without the...

13. Antibodies 101: Epitope Availability

    Type

    Blog Post

    Published

    Jan. 11, 2022, 2 p.m.

    ...instead of when it is attached to a cell membrane.  Picking the Right Antibody  It can get even more complex...

14. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type

    Blog Post

    Published

    Sept. 14, 2021, 1:15 p.m.

    ...recombined product tend to grow faster, so we recommend picking the smaller colonies which are more likely to carry...

15. Don’t Wait To Launch Your Health Science Startup

    Type

    Blog Post

    Published

    Aug. 25, 2015, 2:30 p.m.

    ... your entire career in science, you are likely lacking the necessary business background and/or connections...

16. Advice for Moving Into Sales After Your Science Postdoc

    Type

    Blog Post

    Published

    Aug. 21, 2014, 3:15 p.m.

    ...we make, my job choices were mostly by chance. Luckily, around the time I decided to look for work outside...

17. Hot Plasmids: FRET-Based Biosensors

    Type

    Blog Post

    Published

    May 6, 2014, 1:07 p.m.

    ...firing in neurons and high resolution functional tracking of T lymphocytes, too, making them a versatile...

18. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type

    Blog Post

    Published

    Nov. 27, 2018, 2 p.m.

    ...research related to space medicine. Now he aims on tracking pollution in rivers by employing synthetic biology...

19. 25 Work From Home Tips for Scientists

    Type

    Blog Post

    Published

    March 19, 2020, 1:15 p.m.

    ...for sleep. At Addgene, our mindfulness group is sticking with their Headspace practice even while working...

20. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type

    Blog Post

    Published

    July 27, 2017, 1:11 p.m.

    ...learn more about Xin and Fatima's work and keep checking back in with the Addgene blog for future award...