We narrowed to 285 results for: cki;
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TypeBlog PostPublishedMay 21, 2020, 1:15 p.m....overcome this challenge in Drosophila require heat-shocking the system or using chemical inducers of gene ...
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Oh, The Places You Can Go: Careers in Science Communication - Science Writing
TypeBlog PostPublishedOct. 3, 2017, 12:11 p.m.... writing group I am part of is responsible for checking the scientific accuracy of everything that goes... -
The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place
TypeBlog PostPublishedJan. 21, 2020, 2:30 p.m....individual screens curated for the publication. Clicking on the screen name takes users to a display page... -
Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene
TypeBlog PostPublishedJuly 2, 2015, 8:37 p.m.... days talking to researchers about their work, picking up plasmid deposits, and trying to learn ways Addgene... -
Choosing Your Perfect Empty Backbone
TypeBlog PostPublishedAug. 19, 2014, 3:39 p.m....to be assayed (e.g. pPD95_86 and pPD57_56), or knocking down YGOI through RNA-mediated interference (e.g... -
Choosing a Good Mentor for Scientists
TypeBlog PostPublishedJan. 16, 2014, 2:56 p.m....advisors learning how to be a scientist. It is shocking how little pre-work most PhD students and postdocs... -
Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools
TypeBlog PostPublishedSept. 18, 2018, 1:33 p.m....Quantification of editing efficiencies simply involves tracking the ratio of mCherry-positive to eGFP positive... -
Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation
TypeBlog PostPublishedDec. 13, 2018, 1:14 p.m....Clathrin-mediated endocytosis and subsequent endo-lysosomal trafficking of adeno-associated virus/phage." Journal of ... -
Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog PostPublishedJune 2, 2020, 1:15 p.m....injection of precise titrations of AAV or heat-shocking the organism. The SPARC system uses competitive... -
Meet the New Addgene Board Members!
TypeBlog PostPublishedApril 28, 2020, 1:14 p.m....Library, a library that “enables the high-resolution tracking of more than 1 million cancer cells under drug... -
Antibodies 101: Epitope Availability
TypeBlog PostPublishedJan. 11, 2022, 2 p.m....instead of when it is attached to a cell membrane. Picking the Right Antibody It can get even more complex... -
Three Tips for Preventing Viral Plasmid Recombination in Your Samples
TypeBlog PostPublishedSept. 14, 2021, 1:15 p.m....recombined product tend to grow faster, so we recommend picking the smaller colonies which are more likely to carry... -
Viral Vectors 101: What is a Virus (and a Viral Vector)?
TypeBlog PostPublishedAug. 18, 2023, 4:15 p.m....just on the disease front. Viruses survive by hijacking the machinery of the cells they infect. Once they... -
Antibodies 101: Epitope Tags
TypeBlog PostPublishedMay 11, 2023, 1:15 p.m....application. Depending on the tag, directly imaging or tracking your protein may also be possible, without the... -
New and Upcoming Viral Vectors - December 2019
TypeBlog PostPublishedDec. 19, 2019, 2:40 p.m....pAAV-hSyn-DIO-EGFP Biosensor AAV Calcium sensors Tracking calcium dynamics that govern such activities as... -
Don’t Wait To Launch Your Health Science Startup
TypeBlog PostPublishedAug. 25, 2015, 2:30 p.m.... your entire career in science, you are likely lacking the necessary business background and/or connections... -
Advice for Moving Into Sales After Your Science Postdoc
TypeBlog PostPublishedAug. 21, 2014, 3:15 p.m....we make, my job choices were mostly by chance. Luckily, around the time I decided to look for work outside... -
Hot Plasmids: FRET-Based Biosensors
TypeBlog PostPublishedMay 6, 2014, 1:07 p.m....firing in neurons and high resolution functional tracking of T lymphocytes, too, making them a versatile... -
Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors
TypeBlog PostPublishedJuly 27, 2017, 1:11 p.m....learn more about Xin and Fatima's work and keep checking back in with the Addgene blog for future award... -
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS
TypeBlog PostPublishedJuly 21, 2017, 12:48 p.m.... YFG. You could compare this mutant fly to one lacking the mutation in the same genetic background. In...