We narrowed to 245 results for: construct
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TypeBlog PostPublishedJan. 24, 2019, 2:24 p.m....#104110 - #104112) (Figure 2). We’ve also created constructs with containing chemical tags such as the SNAP-tag...neurons for light microscopy-based neuronal reconstruction. To achieve stochastic multicolor labeling,...
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HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides
TypeBlog PostPublishedOct. 10, 2019, 12:41 p.m....Frankenbody,” like the infamous frankenstein, was constructed by grafting different parts together. By combining... -
Identifying Sequence Elements with SnapGene's Feature Database
TypeBlog PostPublishedDec. 21, 2017, 2:06 p.m....done painstakingly by hand. Records of plasmid construction were often incomplete or nonexistent. In the... -
Multifunctional Neural Fibers: Modulation from Gut to Brain
TypeBlog PostPublishedAug. 31, 2023, 1 p.m....live animal. They started by delivering an AAV construct expressing channelrhodopsin 2 (ChR2), a blue light... -
Another Pathway into Cells: iTOP
TypeBlog PostPublishedJune 23, 2015, 8:37 p.m....primary cells. What if, instead of supplying a DNA construct, they could simply induce cells to take up protein... -
Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]
TypeBlog PostPublishedApril 7, 2015, 4:21 p.m....are comparatively small allowing multiple sgRNA constructs to be introduced simultaneously in a single plasmid... -
Hot Plasmids: FRET-Based Biosensors
TypeBlog PostPublishedMay 6, 2014, 1:07 p.m....additional color variants. This resulted in the construction of redCALWY-1, a red-shifted FRET sensor for... -
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog PostPublishedJune 28, 2018, 3:01 p.m....however, suffer from many pitfalls: they require construction of plasmid vectors and/or rely on cumbersome...be directly purchased and there is no need to construct plasmid vectors or synthesize/purify IVT sgRNA... -
Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond
TypeBlog PostPublishedJune 1, 2021, 1:15 p.m....an individual effort and more of a collective construction. In helping today and be helped tomorrow. Our... -
Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools
TypeBlog PostPublishedDec. 10, 2019, 1:58 p.m.... voltage changes in worms and have made these constructs available at Addgene. These include Arch(D95N... -
Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish
TypeBlog PostPublishedNov. 27, 2018, 2 p.m....response. To create this bacterial sensor, he will construct plasmids containing promoter regions that activate... -
DAP arrays for multiplex gene editing
TypeBlog PostPublishedJuly 7, 2022, 1:15 p.m....sequences can be used together in DAP array. To construct DAP array for multiplex gene editing applications... -
New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor
TypeBlog PostPublishedApril 7, 2022, 1:15 p.m....Sequence schematics for PDGFR, GPI, and SGZ display constructs. Image adapted from Aggarwal et al… Figure 4.... -
Multiplexed Overexpression of Regulatory Factors (MORF) collection
TypeBlog PostPublishedMarch 30, 2023, 1:15 p.m....with each cell receiving only 1 TF expression construct. Cells were cultured following a differentiation... -
Addgene’s Viral Service - Why Virus? Why Now?
TypeBlog PostPublishedMarch 23, 2017, 2:30 p.m....and New England Biolabs sponsored the capital construction of our new BL2+ laboratory and the infrastructure... -
5 Great Apps for Lab Life
TypeBlog PostPublishedSept. 22, 2016, 2:30 p.m....England BioLabs In my lab we do a lot of genetic construction and cloning experiments. Not all of our lab ... -
3 Challenges in Plant Synthetic Biology
TypeBlog PostPublishedJuly 22, 2014, 5:46 p.m.... methods have also been patented. In order to construct our color changing flower we are carefully making... -
Will You Be My Mentor? Finding and Asking for Mentoring Support
TypeBlog PostPublishedFeb. 4, 2014, 3:50 p.m....means the mentee needs to be open to change and constructive suggestions and feedback. The best thing to ... -
Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics
TypeBlog PostPublishedMarch 3, 2020, 2:15 p.m....multiple DNA fragments. This system has been used to construct plasmids for use in model organisms such as E.... -
Hot Plasmids - May 2022
TypeBlog PostPublishedMay 26, 2022, 1:15 p.m....sinus vein injection of a CAG-driven mNeonGreen construct in mouse. Image from Goertsen D, et al., 2022....