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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 291 results for: fus

Showing: 141 - 160 of 291 results

  1. Hot Plasmids - May 2022

    Type
    Blog Post
    Published
    May 26, 2022, 1:15 p.m.
    ...2021.    BLITZ uses the biotin ligase TurboID fused to a conditionally stabilized GFP-nanobody rather...rather than a specific protein of interest. This fusion protein can then be co-expressed in essentially any...

  2. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ...cryopreserved hybridomas, immortalized cells created by fusing an activated B cell that produces the antibody ...light (VL) and variable heavy (VH) chain sequences. Fusion PCR was performed to create an amplicon that  was...

  3. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    Published
    March 21, 2023, 1:15 p.m.
    ...unit. Each particle has a viral envelope, which fuses with the target cell’s membrane, allowing the viral...determine what type of cell the virus will target and fuse with. In nature, this determines which organisms...

  4. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    Published
    Nov. 10, 2022, 2:15 p.m.
    ... Screens and selections The common goals The confusion in differentiating between a screen and a selection...inappropriately categorized as screens! Why the confusion? The commonality between these methods is that...

  7. Click Biology: How to Click and Play to Explore Biology

    Type
    Blog Post
    Published
    Oct. 21, 2025, 1:15 p.m.
    ...parameters of the coupling system include: Wide fusion-tolerance — Click Biology tags should be similarly...organisms, targeted to particular compartments, or fused to other effectors from nanobodies to fluorescent...

  9. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...   Prior to the polyhistidine tag, alternative fusion tags were used, such as S. aureus’ Protein A (Moks... to scientists. If their protein of interest is fused to polyhistidine, then they can use an anti-HIS ...

  10. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    Published
    Nov. 21, 2019, 1:53 p.m.
    ...I suspected I knew at least one source of the confusion in our meetings. A lot of our LIMS planning involved...can help the team identify possible points of confusion in the future. Improving cross team communication...

  11. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ... with soluble fluorescent proteins or localized fusion proteins and do not require dedicated plasmids ...performed on purified fluorescent proteins. To avoid diffusion, the proteins are (i) trapped in microdroplets...

  12. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...orthologs NmCas9 and St1Cas9. Each ortholog was fused to a different fluorescent protein to create three... recruit fluorescent proteins BFP, GFP, and RFP fused to the respective hairpin binding domain. Pairs ...

  13. Developing Lab Management Software for Biology

    Type
    Blog Post
    Published
    Aug. 25, 2016, 2:30 p.m.
    ... as clear as you can be (there will always be confusion about this though) about the fact that containers...them to their contents might leave you sad and confused when a tricky procedure failed and one of your...

  15. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...varied as it can have important implications for the fusion protein effectivity), and a fluorescent protein...degradation the Ward lab used fluorescent proteins (FPs) fused to the AID* (minimal 44 amino acids auxin inducible...

  16. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    Published
    Aug. 1, 2023, 1:15 p.m.
    ...Once you input a sequence, SplitSet finds optimal fusion sites to split it into multiple fragments. You ...windows” you’d like the tool to use to identify fusion sequences for either a circular or linear assembly...

  18. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ...(SP) to carry catalytically dead SpCas9 (dCas9) fused to the bacterial polymerase subunit ω. This construct...flexible. Like the xCas9 3.7 nuclease, xCas9 3.7 fused to third generation cytidine base editor architecture...

  20. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...envisioned a two-component RNA editor: a Cas13 enzyme fused to an RNA adenosine deaminase (ADAR). Such a system...in RNA, creating a functional A->G change. They fused ADAR deaminase domains (ADARDD) to dPspCas13b, but...
Showing: 141 - 160 of 291 results