We narrowed to 513 results for: gats

...repressible promoters can be regulated via positive or negative control. Positive Repressible: An activator protein...sequence anymore and transcription is turned OFF. Negative Repressible: A repressor protein is present, but... as sphingomonads and bacillus.  ADH1 The ADH1 negative repressible promoter is commonly used in yeast...that promoter is active. In this way, you can interrogate the activity of uncharacterized promoters. Similarly...

...can be particularly useful for sharing negative data. Negative data often goes overlooked, but knowing... not encoding this protein, which we will call Negative Control Gene (NCG) (A). Then you lyse the cells...antibodies, as well as a third unrelated antibody as a negative control (A). The two antibodies to your protein...

...account name in the top right corner of PubMed to navigate to your account settings. Next, click on the link...Preferences” box. Once on the Preferences page, navigate to “PubMed Preferences” and click on the “Filters... deposited plasmids. Now that you know how to navigate to the PubMed Preferences and Filters pages, be...

...is, of course, interesting to parse through, aggregation of this information can provide valuable insight... started to explore how I could visualize the aggregated data. Plotting the data in interactive figures...Since Addgene encourages the public sharing of aggregated data only, information on requesting PI(s) & ...

...love negative data, and in fact, what we're most hoping to see from the community is your negative data...the data when an AAVs doesn't work. Sharing your negative data helps others to design better experiments...

... just above it can help you quickly and easily navigate to educational resources, tools, collections in...options into a single place, so you can quickly navigate around the Addgene website without scrolling up...disabilities will find Addgene’s website easier to navigate, thanks to design choices informed by Web Content...

... of starting material; Gibson assembly or Golden gate assembly use a more direct approach, allowing scientists... a lipopolysaccharide of the cell wall of Gram negative bacteria like E. coli that affects tissue culture...differences between transformation, transduction, conjugation, and transfection Resources on Addgene.org ...

... of scientists. 9. Plasmids 101: Positive and Negative Selection for Plasmid Cloning by Jennifer Tsang... (ex: antibiotic selection and auxotrophy) and negative selection (ex: toxin-antitoxin systems and sacB...101: Transformation, Transduction, Bacterial Conjugation, and Transfection by Alyssa Cecchetelli The ...

... is no way a manager can be efficient without delegating and empowering the team to solve problems together...together (tactics for effective delegation will be covered in a future post in this series). Members ...at formal communications events or at informal gatherings, like at lunch. Many experts recommend using ...

...the inside of the neuron more positive or more negative.  Neuronal stimulation: Depolarization When the...Conversely, when the inside of the neuron becomes more negative it becomes less likely to fire and is said to ...hyperpolarized. The chloride pump NpHR brings in negative chloride ions which hyperpolarize the cell. It...

...we'll focus primarily on antibiotics against Gram negative bacteria. In future posts, we'll detail selection...ribosomal subunit; inhibits protein synthesis (elongation step) Bacteriostatic 10 ug/mL Chloramphenicol...inactivates or completely removes (in the case of Gateway cloning) one cassette. Counterselection allows ...

...next generation the CRISPR construct needs to be segregated away to prevent continued production of new alleles... are identified by a phenotype caused by the segregating mutation. Selecting these plants is a learned...integrated into a CRISPR system, then tracking the segregation of the CRISPR construct could be achieved much...

.... These studies used SMRT sequencing, TA-based ligation and tagmentation-based methods for AAV genome ...works for self-complementary AAVs and the TA-based ligation and tagmentation preparation approaches described...AAV genome is single-stranded and both TA-based ligation and tagmentation require a double-stranded template...

... positive), with these puncta turning red (GFP negative/RFP positive) upon fusion of an autophagosome ...RFP-tagged LC3∆G. Like the unmodified LC3, GFP-LC3 is conjugated to phosphatidylethanolamine (PE) and recruited...autophagy. The RFP-LC3∆G, however, can not be conjugated to PE due to a C-terminal glycine deletion, so...

...2016 Addgene’s research team has been actively investigating ways to improve viral vector production. Whether...Whether redesigning viral packaging plasmids, investigating different viral production strategies or developing...method to improve AAV yields. Although we only investigated 3 serotypes it’s possible that this approach...

...product and vector ligate; however, it can potentially rebind and nick the newly ligated DNA. Salt helps ...an extremely large insert. Since the standard ligation reaction is fairly quick, make sure you stay organized...

...NotI sites to Your Gene of Interest (YGOI) for ligation into a recipient plasmid. Designing primers for... In our example, we will use EcoRI and NotI to ligate our cDNA into the recipient plasmid. Remember to...final Reverse Primer sequence of 5’-TGCTTAGCGGCCGCTCAGTACTTCGAGATATGCCA-3’. Preparing the PCR product for...

... Facultative anaerobe Facultative anaerobe Obligate aerobe Number of chromosomes 16 3 4 ... is a non-pathogenic yeast that operates as an obligate aerobe. It’s also a methylotropic yeast, meaning...DOI: 10.3892/br.2017.941 Bernauer L., et al., Komagataella phaffi as Emerging Model Organisms in Fundamental...

...for PZA-resistant Mycobacteria tuberculosis. Investigation of multispecies biofilm formed by Pseudomonas...compounds on these proteins will be tested. Investigating changes in neuronal activity after ischemic ...Armenia Gohar Tsakanova is a Senior Researcher who investigates the molecular and cellular etiopathomechanisms...

...conformational changes in proteins? How can information propagate through a structured material over a long distance...amplified tens or hundreds of thousands of times by propagating through an enormous lattice of identical subunits...