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Addgene
Showing: 141 - 160 of 377 results

  1. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...300,000 individual nucleotide sequences. More than half of these sequences were generated by Addgene as ...on-site, but now we were presented with a new challenge: automating the bioinformatics steps needed to...

  2. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    Published
    July 25, 2024, 1:15 p.m.
    ...antibodies. Assay development, however, can be challenging since scientists must first identify two independent...Longer protocolIncreased chance of cross-reactivityChallenging to develop assay Competitive Appropriate...

  3. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...earlier Voltron indicators (a fusion of Ace2N and HaloTag, Figure 4), they determined that an A122D mutation...changing the dye that becomes conjugated with its HaloTag domain.  Find Voltron2 plasmids here! Abdelfattah...

  4. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ...counter-intuitive, but I find office work more mentally challenging than lab work at times.” Sometimes a desk job...to non-scientists can be a fun and gratifying challenge. You’re also getting the opportunity to learn ...

  5. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    Published
    April 19, 2018, 1:25 p.m.
    ...relationships. Personally, I find this to be the most challenging and time-intensive part of relationship building...research-intensive careers. In addition, this skill is a hallmark for jobs focusing on: Science Policy, Business...

  6. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...from flow cytometry experiments can be a little challenging to understand if you’ve never done any flow. ...., Sait, K., Anfinan, N., Al-Wasiyah,  mohammad khalid, Huwait, E., Gari, M., & Al-Qahtani, M. (2018)....

  7. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    ...transgenic hairy root lines in tissue cultures takes half a year, and generating a transgenic plant can take...Luciferases usually have shorter mRNA and protein half-lives then GUS and are therefore good temporal reporters...

  9. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...suited for recombinant protein expression. Many challenges can arise when over-expressing a foreign protein...functional RNaseE which results in longer transcript half-life F- ompT lon hsdSB (rB- mB-) gal dcm rne131...

  10. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ... the body or self antigens, and toxins that are lethal to animals.  In the clinic, scFvs’ size also provides...plasmid in bacteria (Ahmed et al., 2012). One challenge of bacterial expression is the proper formation...

  13. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ...double strand break repair, it has proven more challenging to develop high efficiency CRISPR-based approaches...delivering CRISPR components as RNPs and delivering two halves of a repair template spread across two AAVs, it...

  14. Transferable Skills Guide: Time Management

    Type
    Blog Post
    Published
    Dec. 5, 2017, 1:52 p.m.
    ...experiment, but you’ll only spend half of each day on it. The other half of each day you can spend accomplishing...

  15. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ...and significance, these receptors have proved challenging to study. Cells often express multiple integrins...adhesion. The results surprised the investigators, challenging the long-held belief that αVβ3 would be the most...

  17. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...important and abundant neurotransmitters, but it is challenging to monitor because only a small number of glutamate...to specific cell types in the brain can be a challenging — but critical — factor for successful experiments...

  18. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...nn1525 Gunaydin, L. A., Yizhar, O., Berndt, A., Sohal, V. S., Deisseroth, K., & Hegemann, P. (2010). Ultrafast... Augustin, S., Sauter, K., Papagiakoumou, E., Gottschalk, A., Soba, P., Emiliani, V., Engel, A. K., Hegemann...

  19. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    Published
    Oct. 2, 2018, 12:56 p.m.
    ...Consider talking to people in the lab across the hall or in the same department as well. What do they ... you? Maybe you like things a bit hectic and challenging and this drives your best work. Or is it teamwork...

  20. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...Cre-dependent conditional alleles, has remained a challenge. Enter Easi-CRISPR: a method that harnesses the...template. Although effective, ivTRT may be more challenging for sequences with complex secondary structure...
Showing: 141 - 160 of 377 results