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Showing: 141 - 160 of 455 results

  1. Sensing Neuronal Dopamine

    Type
    Blog Post
    Published
    July 30, 2019, 12:59 p.m.
    ... famous role as the “happy hormone,” it’s also involved in reinforcement learning, decision making, and...dopamine and other biosensors in our AAV vector inventory. References Darvesh, Altaf S., et al. "In vivo...

  2. Build Your CRISPR Vocabulary

    Type
    Blog Post
    Published
    July 2, 2024, 1:15 p.m.
    ...strand. Protein Cas: Different CRISPR systems involve different proteins, but many of them are called...require a tracrRNA. sgRNA: “Single-guide RNAs” were invented by synthetic biologists. An sgRNA combines a crRNA...

  3. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    Published
    May 4, 2023, 1 p.m.
    ...discovery takes one of two approaches. The first route involves identifying a gene/pathway to develop a drug for...sometimes even genetic pathways that are worth investigating for drug development. Due to the difficulty ...

  4. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    Published
    Dec. 3, 2020, 2:15 p.m.
    ...two genes are found between the AAV genome’s two inverted terminal repeats (ITRs); but when recombinant ...through a pore in the capsid. Rep proteins are also involved in the transcriptional regulation of the AAV p40...

  5. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ...) The transposon consists of a mirrored set of inverted repeats (green) flanking the gene of interest ... enzyme is expressed (red stars) and binds the inverted repeats (green); and an endonuclease reaction ...

  6. 10 Ways to Share Your Science!

    Type
    Blog Post
    Published
    May 13, 2014, 8:34 p.m.
    ...Remember that people like a good story and science involves characters – in this case you – on a quest to ... and hopefully discover something new. Science involves success and, more often, failure. It’s useful ...

  7. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    Published
    Nov. 17, 2016, 3:30 p.m.
    ...be a frustrating process that requires a large investment of time and resources for labs that don’t have...are used in experiments other than just those involving CRISPR and so we also have lentivirus for delivering...

  9. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...determine the binding kinetics of a purified protein involves immobilization on a solid support. This allows...selectivity A common strategy to biotinylate proteins involves amine-reactive biotin derivatives that generate...

  10. The Strength of Story Telling

    Type
    Blog Post
    Published
    Dec. 19, 2017, 2 p.m.
    ...identifying with a character, building emotional investment and following cause-and-effect relationships ...address far and wide, keep your website up to date, invite people to ask questions, be approachable. Engage...

  11. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    Published
    March 26, 2019, 1:40 p.m.
    ...service in 2016, we’ve added many new tools to our inventory of ready-to-use viral vectors. Here are some of...far in 2019. You can also browse our entire AAV inventory! Our new AAVs include: pGP-AAV-syn-FLEX-jGCaMP7b-WPRE...

  12. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    Published
    July 21, 2022, 1:15 p.m.
    ...science communication. The first year of the course involved a whirlwind of skill acquisition. Classes covered...utilize a wide array of media in doing so—can prove invaluable in the pursuit of public engagement. Studying...

  13. RUBY-Red Siliques

    Type
    Blog Post
    Published
    March 1, 2022, 2:15 p.m.
    ...proteins. Selection using resistance genes usually involves killing the untransformed seedlings or callus ...., Zhang, T., Sun, H. et al. A reporter for noninvasively monitoring gene expression and plant transformation...

  15. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    Published
    June 9, 2016, 2:30 p.m.
    ...prokaryotes, and some of these proteins protect against invading DNA. In 2014, Swarts et al. characterized Argonaute...hypothesized that base removal helps prevent the invading genome from “recovering” its original sequence...

  16. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    ...Do you think there are topics we’re missing? We invite you to suggest topics you’d like to see on the ...Cre-lox Cre recombinase can be used to generate inversions, deletions, and translocations. It also provides...

  18. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ...not as comprehensive as this one, so it is an invaluable tool to be used in this capacity. Similar to ...individual plasmids from the library to further investigate specific genes and TF isoforms. Due to the size...

  19. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    Published
    March 24, 2016, 2:30 p.m.
    ...followed these experiments with more detailed investigations into the release of DNA post-cut. Using substrate...Postdoctoral Researcher in Jacob Corn’s lab investigating DNA repair pathways.     Additonal Corn Lab ...

  20. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    Published
    June 24, 2014, 3:59 p.m.
    ...luciferase-containing plasmid is most often used to investigate the effect of regulatory elements, such as promoters...Pathways Known cellular signaling pathways can be investigated in a high-throughput screen using luciferase...
Showing: 141 - 160 of 455 results