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TypeBlog PostPublishedApril 1, 2014, 5:33 p.m....Tom Ellis 1. Keep up with the literature. He recommends setting up a few Pubmed email alerts for new ... have goals outside of the university? Ellis recommends that a student interested in leaving academia...
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Year of the Camelid: Antibody Style
TypeBlog PostPublishedMay 9, 2024, 1:15 p.m....camelid-derived single-domain antibodies (sdAb), commercially known as nanobodies (Arbabi-Ghahroudi, 2017)...antibody and single-domain antibody, under the commercial "Nanobody" name. We’ve talked about sdAbs... -
Embracing Serendipity: A Crucial Element in the PhD Journey
TypeBlog PostPublishedApril 25, 2024, 1:15 p.m....seminars exposes you to discussions beyond your immediate field, fostering a diverse range of perspectives...current project. Another strategy could entail immersing yourself in diverse literature. By delving into... -
Chemogenetics vs. Optogenetics: Which Method Should I Choose?
TypeBlog PostPublishedJune 11, 2020, 1:34 p.m....correct wavelength of light. This response is immediate, on the scale of milliseconds, and can precisely... As a result, it is not possible to elicit an immediate response or track the exact time of activation... -
Antibodies 101: Affinity Tags
TypeBlog PostPublishedSept. 19, 2023, 1 p.m....protein of interest. Many of these tags also have commercially available antibodies, resins, beads, and other...can be removed via protease cleavage at sites immediately following the tag. Unlike MBP, the GST tag can... -
Early Career Researcher Toolbox: Free Online Molecular Biology Tools
TypeBlog PostPublishedSept. 12, 2023, 1:15 p.m....for plasmids assembled from publications and commercially available sources, as well as those deposited... not listed in today’s post? Share them in the comments below. This post was written in April 2020 by ... -
Networking and Inspiring Young Scientists at Freshers Fairs
TypeBlog PostPublishedNov. 17, 2015, 3:30 p.m....society also organises PhD information events and summer internship information sessions to help undergraduate...provide during your education? Let us know in the comments section below! Do you know of a society or event... -
Safe Port in a Storm...How Addgene is Weathering the Pandemic
TypeBlog PostPublishedSept. 17, 2020, 1:15 p.m....-19 research, cures, and diagnostics. Almost immediately we began to receive urgent requests for access...make most of our payroll for the spring and early summer months. We are also extremely grateful to the grant... -
Meet the New Addgene Board Members!
TypeBlog PostPublishedApril 28, 2020, 1:14 p.m....HMS and has been involved in a variety of commercialization efforts in gene therapy either through direct...industry collaborations, grants, or setting up commercial and nonprofit companies. His lab focuses on gene... -
Science Careers: Unruly Interests Feed Many Paths
TypeBlog PostPublishedSept. 26, 2017, 12:12 p.m....avenue with projects studying developmentally programmed regulation of DNA replication and gene transcription... to build skills and connections. AAAS offers a summer program that gives graduate students an opportunity... -
Three Tips for Preventing Viral Plasmid Recombination in Your Samples
TypeBlog PostPublishedSept. 14, 2021, 1:15 p.m....section on a plasmid webpage for any specific recommendations from the depositing lab. If your vector is ...recombined product tend to grow faster, so we recommend picking the smaller colonies which are more likely... -
Plasmids 101: TOPO Cloning
TypeBlog PostPublishedOct. 27, 2016, 2:30 p.m....and releasing topoisomerase (2). These days commercially available TOPO kits provide vectors or cloning...When incubating at room temperature, it is not recommended that you exceed the 5 minute time limit (lower... -
Don’t Wait To Launch Your Health Science Startup
TypeBlog PostPublishedAug. 25, 2015, 2:30 p.m....Being an entrepreneur is difficult; it requires an immense amount of self-knowledge and an unwavering devotion... process in order to develop those ideas into commercial products and next generation companies. We launched... -
Hot Plasmids: FRET-Based Biosensors
TypeBlog PostPublishedMay 6, 2014, 1:07 p.m....change can be observed under a microscope using commercially available beam splitters. In addition to imaging...tendency to shy away from ratiometric imaging, he recommends they give it a shot. “Beginners sometimes think... -
Plasmids 101: NGS Quality Control for Pooled Libraries
TypeBlog PostPublishedOct. 26, 2017, 1:59 p.m.... this is less of a concern, but Addgene still recommends plating the transformation mixture on solid media...interest about library use, let us know in the comments! Additional Resources on the Addgene Blog Check... -
Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors
TypeBlog PostPublishedJuly 27, 2017, 1:11 p.m....launched the Michael Davidson and Roger Tsien Commemorative Travel Awards. These awards are intended to ... first two Michael Davidson and Roger Tsien Commemorative Travel Awardees: Xin Zhou and Fatima Enam. There... -
Viral Vectors 101: Inverted Terminal Repeats
TypeBlog PostPublishedOct. 19, 2023, 1:15 p.m.... with biorender.com. ITR structure ITRs are symmetrical 145-nucleotide sequences that flank the ends ...through ITR sequences. GC tails are often located immediately outside of the ITRs in the plasmid backbone and... -
Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond
TypeBlog PostPublishedJune 1, 2021, 1:15 p.m.... the project we are focusing on what we call “asymmetric collaborations.” These are transient partnerships...protocol can save weeks of trial-and-error. A recommendation for the correct statistical test can save a... -
Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System
TypeBlog PostPublishedJune 4, 2019, 1:02 p.m....launched the Michael Davidson and Roger Tsien Commemorative Travel Awards in 2017. These travel awards are...this year’s Michael Davidson and Roger Tsien Commemorative Travel Awards: Melissa Chiasson, Elizabeth Unger... -
FlipGFP, a novel fluorescence protease reporter to study apoptosis
TypeBlog PostPublishedMay 21, 2019, 12:10 p.m....Apoptosis or “programmed cell death" plays a pivotal role in an array of biological processes including...PMC3790201. Elmore, Susan. "Apoptosis: a review of programmed cell death." Toxicologic pathology 35.4 (2007...