...molecules that may bind non-specifically to assay components and increase background staining. Direct ELISAs...with binding, and some common antibody buffer components can interfere with conjugates. Make sure to do...adds an antigen-specific detection antibody.  Importantly, the detection antibody and the capture antibody... antibody binding.  We hope that you now feel empowered to choose the best ELISA for your needs. Good ...

...initiate replication. But pSC101 requires a third component: RepA, a protein encoded by pSC101 itself. RepA... are efficient for the host, as no additional components are necessary. Iterons are not the only tool ...compensating for the additional metabolic cost imposed on the host with a beneficial function (usually... DNA purification. On the other hand, they also impose a lower metabolic cost on cells and produce lower...

...interchangeable set of fusogenic and targeting components to reprogram delivery vehicles like lentiviral...DIRECTED particles where the fusion and targeting components are interchangeable. Image from Strebinger et...., 2023, reused under CC-BY 4.0 License. Importantly, even commercial antibodies can be tethered on...in light-regulated LOV-Turbo enables precise spatiotemporal control of proximity labeling in living cells...

...that generates the chromophore (Tsien, 1998). Importantly, this reaction requires molecular oxygen and ...genetically encoded tag and exogenously added components. For a review see Lotze et al. (2016). To date...is used to excite GFP, may also excite several components that are naturally present in cells or media....limitations. Being aware of the limitations is important for the successful use of GFP or any of the other...

...directing. Everyone’s schedules were busy, so it was important for me to let people record at their leisure. ...things like lighting, camera angle, and shot composition.  So back to the drawing board.  Cycle 3: A Return... were still many restrictions in place, most importantly, masks.  Besides the muffled voices beneath masks...out of the way when it was blocking some more important lab work from being done. You can watch the first...

...BioRender.com. Loading controls are even more important for semi-quantitative western blots. No pipetter...independent of these other sources of error. It’s important to validate these assumptions for your particular... 2013). To address this source of error, it’s important to determine the linear detection range of all...indicates higher sensitivity, linearity is the most important feature of a loading control, and both total protein...

...populations were also immune to HIV-1 reinfection. Importantly, no off-target effects were detected. Kaminski...patients. Although this is an exciting finding, it’s important to note that these HIV-1 patients were ART-naive...Cas9. While these results are discouraging, it’s important to note that these experiments were done in cell...propagate and infect adjacent cells. It will be important to determine if CRISPR/Cas9 escape occurs in an...

...the 1980s, is one of the most important ways to spatially and temporally control gene expression, especially... 8 colors. The use of AAV provides spatial and temporal control without the need for germline modification...refinements of the Brainbow technique will provide important insights into the complicated physical organization...

...methylase mutation (GATC) Preparing unmethylated DNA, important when trying to cut with certain restriction enzymes... mutation (CCWGG) Preparing unmethylated DNA, important when trying to cut with certain restriction enzymes...propagating plasmids expressing the ccdB gene (important in Gateway cloning). F-mcrA Δ(mrr-hsdRMS-mcrBC...propagating plasmids expressing the ccdB gene (important in Gateway cloning). F- gyrA462 endA1 glnV44 Δ...

...individual types of GPCRs with greater spatial and temporal control than is achieved by traditional pharmacologic...pathway. Photoswitches are great for teasing out important branch points along a signaling cascade that may... activated. Created with BioRender.com.   Important considerations for using optogenetic tools Once...can wreak havoc on your experiments, so it is important to understand the details of how each tool performs...

... genome, the CRISPR/Cas system makes use of 3 components, a guide RNA (gRNA) of about 125 nt that specifies...gRNA, Cas9, and donor oligonucleotide or plasmid components are brought together and microinjected into either...eggs. Or, to avoid handling embryos ex vivo, the components can be electroporated into the oviduct of pregnant... microinjectionist, who has the strategically important job of delivering the payload! Last but not least...

...some DNA during the gel purification step, it is important to digest plenty of starting material. We recommend...be cut with both enzymes, and therefore it is important that the digest go until completion. The time ...running a gel for purification purposes it is important to have nice crisp bands and to have space to ... your favorite gel purification method, it is important to determine the concentration of recovered DNA...

...scientists with an MSc or higher apply. It was important to weigh the cost to benefit of studying a Masters.... I felt then, and still feel now, that it is important to take advantage of the fact that science is ...manufacturing practice (GMP) and good lab practice (GLP) (important buzzwords in industry) as well as skills in industry...permanent position in academia. It is therefore important for young scientists and PhD students to also ...

...fantastic options for delivering shRNA, CRISPR/Cas9 components, and fluorescent sensors. Retroviral vector ...encoding 1) the lentiviral vector genome which was composed of the wild-type 5’ and 3’ LTRs, the ψ sequence...you clone your gene of interest into and is an important feature for your experiment; it must be chosen...using them.   Regarding biosafety, it is also important to be aware that lentiviral particles are a powerful...

...synthesized by eukaryotic cells. First, the kit components rupture the mycoplasma and then specific enzymes...mycoplasma contamination The best and the most important ways to avoid mycoplasma are by providing proper...viability. Conclusion To sum up, it is always important to prevent contamination rather than eradicating...others when you can and help them understand the importance of preventing contamination. Be aware and cautious...

...the way to the complex multi-TU constructs that compose genetic circuits and metabolic pathways. For many..., developmental regulators, and lots of viral components. This kit is also compatible with the Joint Modular...upon (its ‘basics’ MoClo plant kit buddy). It is important to understand your experimental needs before choosing...studies frequently made comparisons between CRISPR components expressed from different plasmid backbones. They...

...have similar compositions and mechanisms, with the biggest differences being the components present in ...effector; types are based on main function and composition; subtypes and variants are typically based on... lack what you may consider to be an integral component of CRISPR — a nuclease effector. However, IV-C...

...their fast movements. Depending on the sample, a temporal resolution on the millisecond scale may be needed...resolution due to the lack of a requirement for high temporal imaging speeds. Standard fluorescence widefield...sectioning, imaging over large distances and high temporal resolution. Traditionally, this type of sample...technique for many of these samples due to its fast temporal resolution and decreased phototoxicity. Lightsheet...

...it is limited to only 20 bases, or why it’s so important to choose a target sequence with a nearby protospacer-adjacent..., but they improve cutting efficiency and are important for robust activity in cells (Jiang & Doudna, ...correct positioning of the non-target DNA strand is important for stabilizing the active conformation of the...CRISPR 101 eBook  Resources on the Addgene blog Components of CRISPR  xCas9: Engineering a CRISPR Variant...

... Note: hypoxanthine (shown) is the nucleobase component of the nucleoside inosine. Created with BioRender.com...removed the uracil glycosylase inhibitor (UGI) components, which inhibit BER during cytosine-to-uracil ...developed two fusions of a CBE with other BER components: the DNA repair protein rXRCC1, or the DNA binding...both E. coli and C. elegans could be effective components of CGBEs. Their eOPTI-CGBE and cOPTI-CGBE had...