...these DNA samples must also be entirely endotoxin free. Endotoxin is a lipopolysaccharide of the cell wall...for your research Brush up on molecular biology protocols using our video collection ...

...viral trasduction (see the Zhang Lab's Nature Protocols Paper for more information). V2.0 This designation...variants, as well as links to the Zhang Lab's Nature Protocols Publication describing them here. References ...engineering using the CRISPR-Cas9 system." Nature protocols 8.11 (2013): 2281-2308. PubMed PMID: 24157548....

...therapy. The laws behind phage therapy, acceptable endotoxin levels, etc. can vary depending where treatment...information on individual phages. Integrating lab protocols, clinical information, phage research job postings...resources on Addgene.org Find molecular biology protocols here Visit the Addgene careers page ...

...lower illumination settings, lowering photobleaching and phototoxicity concerns and extending the potential...

...Biology Reference Find molecular biology protocols Browse our protocol video collection ...needs 5 great apps for lab life  Sharing your lab protocols using apps to save you time and track your experiments...

...production on a protocol video. Don't worry - no pipettes were harmed in the making of this photo!  Here, I ...stuffing new depositor welcome kits, note taking for protocol video production, and keeping inventory of our...

... involved in locomotion that generates periodic motor commands for rhythmic movements like spontaneous...the overlap between groups.  They found that photostimulating the Kolmer-Agduhr cells was sufficient to ...Kolmer-Agduhr cells on one side of the body required photostimulation in order to drive the full symmetrical response...

...plasmids at Addgene Find protocols and gRNA design tools: List of CRISPR protocols developed by a variety...here for a useful reference.  More FAQs, CRISPR protocols, and gRNA design tools Need more questions answered...

...genetic material next to the species-specific protospacer adjacent motif (PAM). Once the CRISPR/Cas complex...the CRISPR/CAS toolkit by posting generic lab protocols, providing tips from experts in the field, and... A. J Bacteriol. 1987 Dec; 169 (12): 5429–33. Photo: Wikipedia, accessed 25 November 2013. Author: James...

...including a link to a detailed CellTag protocol on protocols.io, code and analysis tutorials on GitHub...Hnf4α, a process that like other reprogramming protocols is inefficient. Cells were sequentially tagged...

...brightest teal fluorescent proteins that were photostable and had a high 480/530 nm emission ratio. They...mWasabi is 1.6-fold brighter than EGFP and is photostable. It also has narrow excitation and emission peaks...Directed evolution of a monomeric, bright and photostable version of Clavularia cyan fluorescent protein...

...migration of a cancer cell line equipped with photodissociable EB1 could be stopped in areas with blue light...manipulating and studying cellular dynamics. The photoinducible control of protein function is more specific...contain a smaller RNA tag, are less prone to photobleaching, and achieve better signal in vivo as compared...

...These kits provide in-depth, ready-to-teach lab protocols and supporting materials such as instructional...conditions, to guiding students through specific protocols, ABRC staff are available via e-mail and phone...Addgene.org Find Plant Vectors at Addgene Find Simple Protocols for Introductory Lab Work MoClo Plants Parts Kit...

...10 kb) or has a low copy number, try following protocols for low copy plasmids. For example, using a longer... intact plasmid. Please see our gel extraction protocol for more details.   Figure 2: An uncut... Find more molecular biology and viral vector protocols Learn about the common lab E. coli strains Find...

...steady stream of papers describing new photosynthetic or photosensory molecules that come from basically ...would be helpful to have molecules with better two-photon properties, so you could activate single cells ...

...range of characteristics (color, brightness, photostability, maturation, oligomerization), many of which...incorporation of biosensors, protein evolution lineages, 2-photon spectra, more elaborate spectrum analysis tools...better way to store and represent within-study photobleaching comparisons.  We very much appreciate feedback...

...concentrating your virus. See our Concentrating AAV Protocol (Step 10) for instructions on how to do this. ...take below but you should read our AAV Production Protocol for additional best practices. Keep your cells...Addgene.org Find Ready-to-Use AAV Preps Find Lab Protocols for Work with Virus Watch Our AAV Purification...

...combine computational processing with biological protocols and revolutionary physical approaches. However...the structure in a similar way to the biological protocol, and assembly designs based around the use of ...commercially available tools and components. Rapid prototyping techniques such as 3D printing, on-demand CNC...

...pseudotyping.   Figure 1: Genome of a prototypical gamma-retrovirus.    Frequently asked questions...mammalian cells using 293T cell-based systems. Curr Protoc Immunol. 2001 May;Chapter 10:Unit 10.17C. doi: ...Research More resources on addgene.org Addgene Protocols: General Transfection The Addgene Retrovirus Guide...

... from it? The image in Figure 1 appears on our protocol page for AAV Purification by Iodixanol Gradient...post.  Here, we are presenting this image on a protocol page on our website, as an instructive step-by-step...chose this alt text: "The left panel is a profile photo of an iodixanol gradient after ultracentrifugation...