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We narrowed to 507 results for: otos

Showing: 141 - 160 of 507 results

  1. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...GFP can exist in a protonated and a deprotonated state (Tsien, 1998). The deprotonated state has an absorbance... and it emits light that peaks at 508 nm. The protonated state, however, does not absorb light at 488 ...

  2. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ...FSC/SSC), the detected photons can be represented through a graph of photocurrent vs. time. The resulting...electric potential that can be applied to the photomultiplier inside the cytometer to increase the electric...-mouse CD8b-PerCP-Cy5.5), which is present on cytotoxic T killer cells but not dendritic cells. Using ...

  3. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    Published
    April 24, 2015, 2:39 p.m.
    ...intensity, caused partially by fluorophore photoinstability, as well as the tendency of fluorophores to...ideal characteristics (low aggregation, high photostability, and high stability after fixation.) From the...cells in which the construct is expressed. The photostability of Brainbow-3.2 is improved due to the addition...of neural circuits, such as connections between motor neurons and the neuromuscular junction. In zebrafish...

  4. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...fiber photometry.  Figure 1: Characterization of iGluSnFR4 in mice. A) Scheme for two-photon (2P...iGluSnFR4s produces larger signal-to-noise ratios in two-photon imaging, making it ideal for video recordings of...after inserting EGFP and provide step-by-step protocols for viral generation and stable cell line creation...

  5. 'Tis the Season to #DeckTheLab

    Type
    Blog Post
    Published
    Dec. 2, 2016, 3:30 p.m.
    ...fabulous photos again this year! Why #DecktheLab? Help us spread some cheer by snapping a photo of you... Festivus— you name it, we want to see it! All photos tagged with #DeckTheLab throughout the month of...

  6. DIY DNA Ladders from Penn State University

    Type
    Blog Post
    Published
    July 14, 2017, 2:30 p.m.
    ...significantly cheaper. When using a homemade plasmid prep protocol, it cost less than $10 to make 1000 lanes worth...ladder? You can find the Tan Lab’s DNA ladder prep protocol here and the pPSU1 and the pPSU2 plasmids are ...Use the Kit-Free Alkaline Lysis Plasmid Miniprep Protocol Learn How to Analyze Your Restriction Digests ...

  7. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    Published
    May 17, 2017, 3:37 p.m.
    ... new ways to learn about science careers, lab protocols, and, of course, plasmids. Today we’re trying ...Domain Constructs Kit Hot Plasmid 4: The Yeast Prototrophy Kit from Markus Ralser's Lab -  Use this kit ...strains.  Listen to Our Segment on The Yeast Prototrophy Kit Special thanks to our sound engineer/producer...

  8. Countdown to Halloween @Addgene

    Type
    Blog Post
    Published
    Oct. 29, 2014, 5:26 p.m.
    ...Halloween festivities for years – searching our photo archives I found pictures back through 2009, though...contests were indeed occurring earlier but that the photo evidence was destroyed to protect the innocent revelers...Facebook and checkout our FB page on Friday for more photo evidence.        ...

  10. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    Published
    Jan. 11, 2017, 8:47 p.m.
    ...viral trasduction (see the Zhang Lab's Nature Protocols Paper for more information). V2.0 This designation...variants, as well as links to the Zhang Lab's Nature Protocols Publication describing them here. References ...engineering using the CRISPR-Cas9 system." Nature protocols 8.11 (2013): 2281-2308. PubMed PMID: 24157548....

  11. Phage Directory: From Phage Therapy to a Repository of Phage Information

    Type
    Blog Post
    Published
    Jan. 10, 2019, 2:38 p.m.
    ...therapy. The laws behind phage therapy, acceptable endotoxin levels, etc. can vary depending where treatment...information on individual phages. Integrating lab protocols, clinical information, phage research job postings...resources on Addgene.org Find molecular biology protocols here Visit the Addgene careers page ...

  12. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ...lower illumination settings, lowering photobleaching and phototoxicity concerns and extending the potential...

  13. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

    Type
    Blog Post
    Published
    July 16, 2019, 12:59 p.m.
    ... involved in locomotion that generates periodic motor commands for rhythmic movements like spontaneous...the overlap between groups.  They found that photostimulating the Kolmer-Agduhr cells was sufficient to ...Kolmer-Agduhr cells on one side of the body required photostimulation in order to drive the full symmetrical response...

  15. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type
    Blog Post
    Published
    June 29, 2023, 7 p.m.
    ...production on a protocol video. Don't worry - no pipettes were harmed in the making of this photo!  Here, I ...stuffing new depositor welcome kits, note taking for protocol video production, and keeping inventory of our...

  16. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    Published
    Nov. 25, 2013, 10:12 p.m.
    ...genetic material next to the species-specific protospacer adjacent motif (PAM). Once the CRISPR/Cas complex...the CRISPR/CAS toolkit by posting generic lab protocols, providing tips from experts in the field, and... A. J Bacteriol. 1987 Dec; 169 (12): 5429–33. Photo: Wikipedia, accessed 25 November 2013. Author: James...

  18. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    Published
    May 9, 2019, 1:15 p.m.
    ...including a link to a detailed CellTag protocol on protocols.io, code and analysis tutorials on GitHub...Hnf4α, a process that like other reprogramming protocols is inefficient. Cells were sequentially tagged...

  19. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    Published
    April 25, 2019, 3:01 p.m.
    ...brightest teal fluorescent proteins that were photostable and had a high 480/530 nm emission ratio. They...mWasabi is 1.6-fold brighter than EGFP and is photostable. It also has narrow excitation and emission peaks...Directed evolution of a monomeric, bright and photostable version of Clavularia cyan fluorescent protein...
Showing: 141 - 160 of 507 results