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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 628 results for: rel

Showing: 141 - 160 of 628 results

  1. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ...that’s only good once. Even when things return to relative normalcy, I’ll be incorporating that mobile monitor... live on set, but peering at a 7-inch wireless monitor. Wireless video technology has gotten very impressive...Besides the muffled voices beneath masks, humans rely on mouths to help them hear and read emotional cues... video: So first things first was getting that wireless HDMI transmitter/receiver, to beam the camera’...could work with that.  Focus: Next up, I found a wireless follow focus system. Follow focus systems allow...Monitor: I then set up a monitor connected to the wireless transmitter and an iPad in the office next door...Figure 4: The remote directing setup, complete with wireless video receiver, camera control, and extra hand...

  2. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...population. Heat maps are often used to indicate the relative density of cells. You can see both of these features...are used to show frequency of events, instead of relying on clustered dots. In a contour plot, each line...intracellular IFNγ, IL-17 and IL-10 producing T cells correlates with the occurrence of post-transplant opportunistic...

  3. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ...could be a matter of timing, as jGCaMP7 was released relatively soon after GCaMP6. It could be a matter of...editor of Addgene’s eBooks — including the recently released Viral Vectors 101 2nd edition — I was incredibly...lessons learned from our plasmid lab to develop a reliable and robust high quality viral vector service, ...vector service because we realized that having reliable ready-to-use vectors made from plasmids in the...

  4. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ...L-arabinose) without signs of toxicity or burden relative to a fluorescent protein control. We also added...to produce GVs in their own lab using the most reliable method, which is the transient cotransfection ...

  6. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    Published
    Oct. 25, 2016, 2:30 p.m.
    ...research on these institutions, we can say with relative certainty that just over 40% of current postdocs...Future of Research, we think this is a good thing. Reliance on a cheap trainee workforce has clearly not been... do so on their own terms. They must feel very relieved not to be going through the urgent scramble of...

  7. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ...these duplications (slightly larger bands sizes relative to the original template). Due to the small difference...plasmid context). The technique is also highly relevant in this age of CRISPR; site-directed mutagenesis...parental plasmid (or other). This is particularly relevant in cases where a large plasmid with multiple functional...

  8. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...Uses of biotin in molecular biology Biotin is a relatively small, water soluble molecule that does not interfere...of some other common applications and links to relevant papers and webpages are listed below: EMSA (Electrophoretic...

  10. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...raw %}{"no_follow":false,"open_in_new_tab":true,"rel":"noopener","sponsored":false,"url":{"content_id"...transfection or viral delivery of a plasmid. RNAi is an umbrella term for a number of different applications that...years before CRISPR burst on to the scene. shRNAs reliably knock down a target gene but have a lot of off-target...

  11. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ...Like other transversion editors, this editor had relatively high indel rates ranging from 4.7% to 30% at ...was on its way! All base editors up to this point relied on deaminase enzymes, either to directly accomplish...

  12. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...technologies nanopore-based DNA sequencing machines are relatively small meaning that DNA sequencing could move ...next-generation sequencing techniques do not typically rely on Sanger sequencing but instead use methods like...

  14. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...the intended edit must be in a 5 - 10 nt window relative to the PAM, and bystander edits are possible if...introduction of a specific mutation, one generally relies on homology directed repair (HDR) to incorporate...along with the single cell clone (although this is rarely done). When targeting a dsDNA break for HDR, the... has been shown that the FANTOM database, which relies on CAGE-seq to directly capture the mRNA cap, provides...

  15. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...long been extremely useful in the lab – they are relatively small and easy to work with, making them the ...the day, March 16, 2014, that I got my first preliminary results from a CRISPR genome editing experiment...

  16. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ... when the genome editing frequency is low, the relative abundance of exon-skipped mRNA is high, likely...and large animal models with mutations in human-relevant exons 45-55, rather than in the traditional exon...

  17. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    .... Speed: AAV transduction experiments are relatively quick. We have found that expression occurs quickly...roles of certain cell types in locomotion. We have relied heavily upon AAV vectors to deliver chemogenetic...AAV selection, there are three important factors related to location. Location (1): What tissue? Location... (2007): 1740-1743. PubMed PMID: 17882145. 2. Zincarelli, Carmela, et al. "Analysis of AAV serotypes 1...

  18. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ... day or a week planning an experiment, even a relatively simple experiment. However, by taking this time...fellowship grants) A project with sufficient preliminary data to establish your own lab and get an independent...Also, it is unlikely that a mentor has the only relevant expertise and perspective for your project. Furthermore...surprising findings when studying a seemingly unrelated problem. However, you have finite time and resources...Present your work often Surprisingly, many PFs are reluctant to give talks or posters about their research....requires evaluation of all of the data and any recent relevant studies in the literature. Plus, presentations...too much practice writing Young group leaders are rarely great writers. There are two ingredients to becoming...

  19. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    Published
    June 8, 2021, 1:15 p.m.
    ...neurodegenerative disease because they had close relatives suffering from similar diseases. Others were inspired...t know yet has helped me cultivate stronger relationships with my labmates. Sometimes what I thought was...

  20. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ...course, many types of teams. Some teams may be relatively permanent; some teams may only need to exist ...
Showing: 141 - 160 of 628 results