We narrowed to 262 results for: rigi

1. Resume Writing for Non-academic Science Careers

   Type

   Blog Post

   Published

   Dec. 18, 2014, 4:06 p.m.

   ... & Astronomy, University of British Columbia. Originally on track for a research career, she transitioned...

2. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

   Type

   Blog Post

   Published

   March 17, 2020, 1:32 p.m.

   ...much higher base editing activity compared to the original TadA-7.10 enzyme. After testing numerous TadA ...

3. Developing a cold tolerant E. coli using specialized chaperones

   Type

   Blog Post

   Published

   Jan. 28, 2020, 2:35 p.m.

   ...a much slower rate under cold temperatures, and frigid conditions decrease the potential for microbial...

4. Targeted Mutagenesis with EvolvR

   Type

   Blog Post

   Published

   Feb. 21, 2019, 1:15 p.m.

   ...targeted mutagenesis rate ~9-fold higher than the original nCas9, while increasing the standard mutation ...

5. 15 Years of Addgene: The Top 15 Plasmids

   Type

   Blog Post

   Published

   Jan. 8, 2019, 1:54 p.m.

   ...Note: this plasmid was called pLKO-Tet-On in the original publication but its name was subsequently changed...

6. Improved Plasmid Maps Powered by SnapGene

   Type

   Blog Post

   Published

   June 29, 2017, 1:06 p.m.

   ...resistance genes, selectable markers, promoters, origins, tags, and certain ORFs. Their sophisticated detection...

7. Luminescent Imaging with Nano-lanterns

   Type

   Blog Post

   Published

   May 25, 2017, 2:30 p.m.

   ... medium to allow RLuc8 to produce photons. The original nano-lantern emitted yellow-green light and exhibited...

8. Multiplex Genome Editing with CRISPR-Cpf1

   Type

   Blog Post

   Published

   May 9, 2017, 2:12 p.m.

   ...crRNA array (bolded red arrow).  This undoes the original reversing of the direct repeats, leading to expression...

9. Screening for Successful Genome Editing with Digital PCR

   Type

   Blog Post

   Published

   March 30, 2017, 2:30 p.m.

   ... number of target DNA molecules present in the original sample with a great deal of precision. This means...

10. How Addgene Creates Content

    Type

    Blog Post

    Published

    Nov. 17, 2022, 2:15 p.m.

    ...scientific support staff receives – in fact, the blog originally started as a way to easily store and share in-depth...

11. Building and Validating Recombinant Antibodies: A Case Study

    Type

    Blog Post

    Published

    April 5, 2022, 1:20 p.m.

    ...including subclasses that were not produced by the original hybridoma. By using subclass specific secondary...

12. Viral Vectors 101: Inverted Terminal Repeats

    Type

    Blog Post

    Published

    Oct. 19, 2023, 1:15 p.m.

    ...plasmid.  Within the AAV genome, ITRs serve as the origin of replication and are required for replication...

13. Viral Vectors 101: Viral Vector Elements

    Type

    Blog Post

    Published

    July 25, 2023, 1 p.m.

    ...cells. In adenovirus, the ITRs serve as a type of origin of replication to initiate replication of the dsDNA...

14. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type

    Blog Post

    Published

    Sept. 26, 2024, 1:15 p.m.

    ...restriction sites or DNA ligase! This post was originally written by Mary Gearing in December 2015 and ...

15. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type

    Blog Post

    Published

    Aug. 6, 2024, 1:15 p.m.

    ...written and updated by multiple Addgenies. It was originally published in 2018, in the Viral Vectors 101 eBook...

16. Fluorescent Proteins 101: Luciferases

    Type

    Blog Post

    Published

    July 22, 2025, 1:15 p.m.

    ...you used? Share your tips below! This post was originally written by Jason Niehaus in June 2014 and was...

17. Viral Vectors 101: The AAV Retrograde Serotype

    Type

    Blog Post

    Published

    April 27, 2023, 1:15 p.m.

    ... forth and trace some neurons!  This post was originally written by Leila Haery in 2017 and significantly...

18. RNA Extraction Without A Kit

    Type

    Blog Post

    Published

    March 24, 2020, 1:15 p.m.

    ...homogenization solution and phenol addition of the original no-kit protocol into one step. After homogenization...

19. Visualizing Protein Turnover In Situ

    Type

    Blog Post

    Published

    Jan. 16, 2018, 3:20 p.m.

    ... clever combination of FUNCAT and PLA PLA was originally published by the Söderberg lab in Sweden as a...

20. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type

    Blog Post

    Published

    Jan. 11, 2017, 3:30 p.m.

    ...inconsistencies across geographical regions When we originally started gathering this information, many assumed...