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We narrowed to 505 results for: sam

Showing: 141 - 160 of 505 results

  1. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...deserve, but, in this post, we'll provide a small sampling of the many amazing new plasmid tools that have... joined modules cannot be separated again by the same cloning enzymes. MXS (MluI-XhoI-SalI) chaining results...photo-label multiple mitochondrial populations within the same cell. Mitochondrial fusion is typically assayed ...different mitochondrial subpopulations within the same cell; by photoswitching PSmOrange and photoactivating... and more, to your protein of interest using the same vector system. The tRNA/aaRS pairs are delivered... experiments in several ways. The laboratory of Samie Jaffrey describes using these tags in an in-gel ...

  2. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    Published
    April 23, 2020, 1:15 p.m.
    ...accommodate adeno-associated viral vector (AAV) samples and created a simplified process, termed viral ...parallel, there is a very real risk of mixing up samples, serotypes, or both. Unfortunately, the standard... as [+] and [-] strands that are packaged at the same frequency, we reasoned that, following DNA extraction...

  3. RUBY-Red Siliques

    Type
    Blog Post
    Published
    March 1, 2022, 2:15 p.m.
    ...GUS staining or are not suited to studying large samples. RUBY Red But recently, something new has been ...consisting of these three enzymes expressed under the same promoter with self-cleaving 2A site linkages and...transformation next to an untransformed seed from the same plant beginning to germinate. (Top right, second...

  4. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    ...endogenous WT levels of the same protein when both are expressed in the same system. Tagging can also aid...EQKLISEEDL) derived from the protooncogene of the same name. The tag has a net negative charge with 4 negatively...

  5. Plasmid Cloning by PCR

    Type
    Blog Post
    Published
    March 29, 2016, 2:30 p.m.
    ...about making a copy of a piece of DNA and at the same time adding restriction sites to the ends of that...restriction enzymes that can both function in the same buffer, as this will save time later In our example...As such, the later steps in this process are the same as those discussed in our restriction cloning post...

  6. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ... DIY Biology and, on occasion, would meet in the same space where BosLab exists today. Back then, it consisted... getting the community to collect various soil samples, extract DNA, sequence it, and analyze the data... characterize the microbiomes of the different samples. The goal is to not only learn something about ...

  8. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    Published
    Sept. 12, 2019, 1 p.m.
    .... Likewise, a lot of scientists have requested samples of AAV for testing purposes. To address this, we... serotype testing AAV, which are small (20 ul) samples of EGFP-expressing AAV packaged in various serotypes... kit with all the serotypes, since these 20 ul samples are distributed individually. See our Serotype ...

  9. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

    Type
    Blog Post
    Published
    Aug. 28, 2014, 3:34 p.m.
    ... The glycerol in the buffer will make sure your sample settles in the gel well and the dyes provide a ...you to interpret the bands that you get in your sample lanes. Choose your ladder based on the expected...approximate the DNA mass of comparably intense samples of similar size. Run the gel at 80-150V until you...

  10. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    Published
    June 1, 2017, 2:30 p.m.
    ...that are related to the imaging strategy and the sample. Practical brightness A protein with a high intrinsic... a reference protein that is translated from the same open-reading frame and separated from the protein...brightness for a set of fluorescent proteins (with the same reference protein) under identical conditions, a...

  11. Antibodies 101: Isotypes

    Type
    Blog Post
    Published
    Oct. 19, 2021, 1:15 p.m.
    ...of antibodies, although not all isotypes have the same ability to activate each of these functions: Recruitment...appropriate isotype control. An isotype control has the same constant heavy chain domain as your primary antibody...results in the B cell expressing an antibody with the same variable domain but a different heavy-chain constant...

  12. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    Published
    Aug. 7, 2014, 1:58 p.m.
    ...opportunity to meet people who are interested in the same things you are on a deep level. Right away you have... and grow over time, but being interested in the same phosphate of your favorite kinase is a good start...meet someone since you are both interested in the same talk. Read more on this advice to “never leave an...

  13. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

    Type
    Blog Post
    Published
    April 23, 2018, 2 p.m.
    ...Factors Podcast Segment Researchers from the El-Samad lab recently deposited plasmids encoding synthetic...encoding these synthetic transcription factors, the El-Samad lab has deposited plasmids encoding fluorescent ...Aranda-Díaz et al. for more information. The El-Samad lab predicts that this level of control will enable...

  14. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...intensive to process a large number of microbiome samples. To enable large-scale, longitudinal studies of...and can approximate mRNA concentration within a sample. Using this system, the Collins lab was able to...lab validated their platform on clinical stool samples by comparison to qPCR and demonstrated that it ...

  15. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...metal ions are immobilized on a bead or resin and a sample containing the protein of interest flowed through...interest and the metal ions.      Fig. 1: A sample containing a polyhistidine tagged protein of interest...tags are also recommended if you need a very pure sample. Because binding strength is proportional to the...

  16. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    Published
    Aug. 6, 2019, 12:55 p.m.
    ...experimental and control mice will have been raised in the same environment and will harbor similar epigenetic modifications...multiple mutations is if the mutations are on the same chromosome. Homologous chromosomes recombine during...two loci, which would get both mutations onto the same chromosome. Until crossing over occurs between the...

  17. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ...ensure the entire team is using the same rubric and making the same decisions when analyzing the wide variety... that is provided, each deposit goes through the same QC process to ensure all plasmids pass our high-...

  18. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ...titers measure the amount of viral particles in a sample and are usually based on the presence of a viral...however, is that a p24 ELISA measures all p24 in the sample regardless of whether or not it is incorporated...lentiviral prep may be significantly higher than the same prep that has been stored in the freezer. If you...

  19. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type
    Blog Post
    Published
    June 22, 2017, 2:30 p.m.
    ...The same way the human body is made up of organs, cells comprise compartments and structures, called ...insights about the proximity of two proteins within the same subcellular structure or protein complex. By coexpressing...dynamic association in live biological fluorescent samples." PloS one 9.4 (2014): e94245. PubMed PMID: 24728133...

  20. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...of the drawbacks of kits and typically follow the same principles: cells are lysed, RNAs and proteins are... centrifugation. At this point, the plasmid DNA sample can be treated with RNase to remove RNA contamination... buffers ran out. Homemade buffers performed the same as those provided with the kit. Although not tested...
Showing: 141 - 160 of 505 results