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We narrowed to 266 results for: uros

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Showing: 141 - 160 of 266 results

  1. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    Published
    May 26, 2020, 1:15 p.m.
    ...stably integrate a reporter gene that contains a puromycin resistance gene followed by the target site (the...genome edit:  A donor plasmid that contains a puromycin resistance gene linked to an in-frame H2B-GFP....

  2. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ... recombinant antibody resource called NABOR (Neuroscience AntiBody Open Resource). The antibodies distributed...of monoclonal antibodies publicly available. The Neuro Mab Sequencing Initiative uses high-throughput DNA...

  4. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    Published
    July 21, 2016, 2:30 p.m.
    ...international, collaborative project—funded by the European Commission, the US National Science Foundation...increasingly, corporations in the US and Western Europe have taken three strategic approaches to gender...

  6. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ...for Gordon Fishell Lab constructs targeting GABA neurons. Serotypes include AAV1, and AAV9. Find viral ...channelrhodopsin (C1V1) targeting parvalbumin cortical interneurons is now available as a ready to use viral prep...

  7. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ... form of blindness known as Leber’s congenital amurosis (LCA). This disease is caused by mutations in ...central and peripheral nervous systems." Nature Neuroscience 20.8 (2017): 1172-1179. PubMed PMID: 28671695...

  8. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...cell types including endothelial cell-pericyte, neuron-astrocyte, and cancer-stromal cell pairs.   ...new additions.  Image calcium transients in PV interneurons with AAV-S5E2-GCaMP6f, now available as a ready-to-use...

  9. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ... morphology, and circuit mapping of a targeted neuronal population.    Figure 2 (modified from...activated and inactivated transgene expression in neurons.  PubMed PMID: 2286602. PubMed Central PMCID: PMC3406316...

  11. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...selectively in Parvalbumin containing cortical interneurons. We now provide ready-to-use AAV vectors expressing...this promoter. Image calcium transients in PV interneurons with AAV-S5E2-GCaMP6f are now available as a...

  12. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...mitochondrial fission/fusion, nuclear import, and neuronal trafficking. Although FPs have enabled discovery...YFP, Strep, TEV-V5) with either blasticidin or puromycin selection. Researchers at the Allen Institute ...

  13. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    Published
    Sept. 7, 2017, 12:17 p.m.
    ...and endocrine cells such as AtT-20, PC12, Rin5F, Neuro2A and GH4C1, among others (see Lindberg papers in...of Medicine. Iris Lindberg is a professor of Neurobiology and Anatomy at the University of Maryland Medical...

  14. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...placing FP timer DsRed-E5 under the control of Neurog3 (a gene controlling pancreatic differentiation)...preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric...

  15. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...A., Smith, C. J., Ottolini, M., Barker, B. S., Purohit, A. M., Grippo, R. M., Gaykema, R. P., Spano, A...magnetic control of the nervous system. Nature Neuroscience, 19(5), 756–761. https://doi.org/10.1038/nn.4265...

  16. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...cells where recombination has occurred. Neomycin, puromycin, and hygromycin resistance genes are commonly ...GFP, as seen in blue flame plasmid OCT4-eGFP-PGK-Puro from the Jaenisch lab.   Figure 3: A knockout...

  17. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...postdoc I’m currently doing an industry postdoc in neuroscience at AstraZeneca’s Waltham site after doing a ... and is applying this towards researching neurodegenerative diseases.   Additional resources on the Addgene...

  18. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...Jake Watson is a postdoctoral researcher in neurophysiology at the MRC Laboratory of Molecular Biology....Laboratory of Molecular Biology. He is interested in neuronal signal transduction, and uses electron cryo-microscopy...

  19. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...Outreach Scientist at LGC Group, London, which is the European distributor for ATCC. He studied biochemistry ... high level of experience he now travels around Europe giving talks and seminars on cell culture and other...

  20. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ...pseudoreplication in neuroscientific studies: is it affecting your analysis? Lazic SE. BMC Neurosci. Jan 14;11:...
Showing: 141 - 160 of 266 results