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We narrowed to 599 results for: REV;

Showing: 81 - 90 of 599 results

  1. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...photostability under two-photon illumination than previous GEVIs. Some constructs are also available as viral...kinetics and photochemical characteristics than previous NIR calcium reporters iGECI and NIR-GECO2G, and...reduced sensitivity to pH and calcium compared to previous lactate biosensors, and its affinity is better-matched...temperature sensor that significantly outperforms previous designs. B-gTEMP is excitable under blue light...B-gTEMP itself is a significant improvement over previous temperature indicators, it also demonstrates that...Intracellular Heat Transfer and Thermal Property Revealed by Kilohertz Temperature Imaging with a Genetically...

  2. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...suggest that phosphorylation is far more prevalent than previously appreciated. PhosphoSitePlus, one of the... group. Additionally, these mutations are not reversible, and thus cannot reflect the true state of protein...Extensive genomic sequencing of prokaryotic lineages revealed a curious anomaly amongst a subset of methanogenic...mutation of these residues. Due to the limitations previously mentioned, phosphomimetic mutations on the activation...affects protein function, researchers should try to reverse the effects of pSer-incorporation on enzyme function...

  3. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    Published
    April 24, 2015, 2:39 p.m.
    ...the continued utility of Cre-lox. Check out our previous blog post, Plasmids 101: Cre-lox, if you need ... removes one of the other loxP site variants, preventing further recombination. Brainbow-1.1 scales up...that transient Cre recombination is required to prevent constant inversion of the construct. Sanes and...their protein sequences are highly homologous, preventing design of antibodies specific to each fluorophore...of delicate axonal and dendritic processes not previously visible with Brainbow-1 and -2. The general structure...connectome. Lichtman JW, Livet J, Sanes JR. Nature Reviews Neuroscience. 2008 Jun;9(6):417-22. doi: 10.1038...

  4. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    Published
    June 5, 2017, 3:19 p.m.
    ...as a nonprofit under the United States Internal Revenue Code 501(c)(3). That means we were formed to benefit... are able to fulfill our mission by generating revenue through distribution of research materials. Our..." is a bit misleading. Nonprofits can generate revenue (as Addgene does through materials distribution... nonprofits should have some level of positive revenue to build reserves so the company is sustainable...The difference is what nonprofits do with their revenue. A nonprofit organization cannot distribute profits...

  5. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    Published
    June 20, 2017, 2:30 p.m.
    ... news is that this effect is reversible and the pH shift can be prevented. Keep this information in mind...if you’re receiving samples on dry ice - it may prevent you from seeing some unexpected results. Order... 3). However, 30 seconds was not sufficient to prevent any pH change, as we still observed a slight change... allowed the CO2 to leave the tubes, and would prevent the pH change upon thaw (Fig.4). To test this, ...

  6. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ...entirely too much work for one person, it would take forever, and the end product would likely be poor quality... delegate the giving of feedback or performance review. You can’t delegate tasks assigned to you and you...outcomes, timelines and deadlines. Agree on a way to review project progress. I recommend putting a regular...approachable mistakes will be made that could have been prevented.  Make sure everyone understands that no question... Aren't You Delegating" in the Harvard Business Review. Another excellent blog on delegation tips and ...

  7. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...your vocabulary! A brief review of base editing tools First, let’s briefly review how CRISPR-derived base...The first base editors revolutionized CRISPR gene editing. Cytosine base editors (CBEs) and adenine base...the pyrimidine bases (C and T). (Check out our previous post on these editors for more details on how ...also called apurinic or apyrimidinic sites and abbreviated as AP sites. To explore this further, the team...bound, keeping the two DNA strands separate and preventing repair based on the complementary strand. This...

  8. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type
    Blog Post
    Published
    Dec. 6, 2018, 1:34 p.m.
    ...sequencing, revealing that they bind to a much larger subset of protein motifs than previously thought. ...T7 terminators adjacent to the T7 promoters to prevent the incorporation of non-specific nucleotides into...proteomics in model organisms in which it was previously not an option, including Drosophila and C. elegans...Pubmed PMID: 30370338.  SH3 domain collection reveals novel peptide ligand motifs Article contributed...

  9. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    Published
    June 15, 2017, 2:30 p.m.
    ...Advanced microscopy, University of Amsterdam. The previous two posts in this series described a practical...between the FPs interfere with native interactions, preventing proper localization in these assays. However,...tendency in living cells. Strikingly, the OSER assay reveals that tagRFP does not act as a true monomer in cells... cells (Constantini, 2012), whereas it was previously concluded to be monomeric based on gel filtration... that are relevant for your research. This will reveal the performance of fluorescent proteins in your...

  10. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type
    Blog Post
    Published
    May 5, 2015, 3:54 p.m.
    ... titles, authors, year, narrow by article type (review, patent, clinical study, research article etc)....on a new literature search, try to find a recent review article to give you an overview of the topic and...information dense and since it continually builds on previous studies, the reader always enters in the middle...introductions should be fairly focused. Consult a review article for a broader scope of what’s going on ...performed that could influence the results? The peer-review process is not perfect, so even though it is science...
Showing: 81 - 90 of 599 results