We narrowed to 466 results for: SHI

...careers, women in STEM, work-life negotiation, and mentorship, we decided to have her participate in an AMA...careers, women in STEM, work-life negotiation, and mentorship. We can direct people to the AMA in the future...whole point is to meet actual people to build relationships. I also like Twitter because I can get a little...important to find good mentors and incentivize good mentorship: AMA mechanics: Be prepared! The mechanics of... mentorship...

...HDR), a distinct cellular DNA repair pathway. Accomplishing this usually involves simultaneously introducing...characterize this phenomenon and found a general relationship between the distance of a mutation from the ...(and let them cut close).   Figure 2: Relationship between cut site to mutation distance and editing...mutations When thinking further about the distance relationship described above, we realized that, in addition...incorporation efficiency, we might exploit this relationship to direct the zygosity of the cells. This was...

...Chapter 1 - Introduction to Mark Springel, Olivia Ho-Shing, and the Journal of Emerging Investigators. Chapter...Chapter 3 - JEI target audience and their current readership. Encouraging students to do science anywhere.... building curricula and developing one-on-one mentorship programs. Chapter 6 and Outro - Interview with...the future. Many thanks to our guests,Olivia Ho-Shing, Mark Springel, Arooba Ahmed, Jiachen Lee, and Jillian...

... questions in the sections below. Mentoring relationships can help identify your priorities and needs,...too, they may not have had experience writing, publishing, or discussing career opportunities with the ...PI removed a woman from the appropriate first authorship position on a publication of her project and ...reminders of blunders, setting target up to fail, shifting goal posts without telling the target Warning...open PI helps lab members determine project "ownership" and author order PI lets lab members fight it...

... colocalization factor to assess the spatial relationship between the targets. Or, it might be sufficient...non-specifically – try optimizing your blocking and washing conditions or consider using a different antibody...concentrations of all the different antibodies, blocking, washing, and sample preparation steps, in order to ensure...getting started with two or three targets or you’re pushing the limits like Addgene depositor Ronald Germain... Speranza, E., Chu, C. J., Gola, A., Thakur, N., Shih, R., Yao, L., Yaniv, Z. R., Beuschel, R. T., Kabat...

...located.  The export license, if granted, allows us to ship rabies plasmids and viral vectors containing genetic...control license, once approved, allows Addgene to ship plasmids or viral vectors containing sequences from... Once the application is approved, Addgene then ships the material following our normal process.   Due...have any questions about this or other Addgene shipping and ordering processes, don’t hesitate to contact...

... scientist. Leadership and management Take a lead from the amazing students at Washington University who...You are finishing your PhD or perhaps you have almost completed a postdoctoral position… or two. You ...in your department, create them and show your leadership skills too. Teach classes Go to workshops and...

...repository. 100,000 Plasmids Shipped! - By 2010, Addgene had hit our stride. We shipped our 100,000th plasmid... 10 years ago today. In that time, Addgene has shipped over 350,000 individual plasmids to 5,000 different...cells into pluripotent stem cells. Plasmids from Shinya Yamanaka's lab and James Thomson's lab were the...

...https://doi.org/10.1126/science.aav7271 Yoshimi, K., & Mashimo, T. (2022b). Genome editing technology ..., Demircioglu, F. E., Moeller, L., Kocalar, S., Oshiro, R., Makarova, K. S., Macrae, R. K., Koonin, E....j.mib.2017.05.008 Makarova, K. S., Wolf, Y. I., Alkhnbashi, O. S., Costa, F., Shah, S. A., Saunders, S. ... S., Wolf, Y. I., Iranzo, J., Shmakov, S. A., Alkhnbashi, O. S., Brouns, S. J. J., Charpentier, E., Cheng...

...absorbed. This phenomenon is known as the Stokes Shift, or the difference in wavelength between peak excitation...background signals when used with the same protein (Hayashi-Takanaka et al., 2014). Other dyes may prove challenging...accurate and reliable imaging and assay results (Hayashi-Takanaka et al., 2014)​​. Conjugates with lower...for intracellular fluorescence live imaging ​​(Hayashi-Takanaka et al., 2014). Dyes can also vary in their..., 987–994. https://doi.org/10.1038/nmeth.4403  Hayashi-Takanaka, Y., Stasevich, T. J., Kurumizaka, H.,...