...Chapter 1 - Introduction to Mark Springel, Olivia Ho-Shing, and the Journal of Emerging Investigators. Chapter...Chapter 3 - JEI target audience and their current readership. Encouraging students to do science anywhere.... building curricula and developing one-on-one mentorship programs. Chapter 6 and Outro - Interview with...the future. Many thanks to our guests,Olivia Ho-Shing, Mark Springel, Arooba Ahmed, Jiachen Lee, and Jillian...

... questions in the sections below. Mentoring relationships can help identify your priorities and needs,...too, they may not have had experience writing, publishing, or discussing career opportunities with the ...PI removed a woman from the appropriate first authorship position on a publication of her project and ...reminders of blunders, setting target up to fail, shifting goal posts without telling the target Warning...open PI helps lab members determine project "ownership" and author order PI lets lab members fight it...

... colocalization factor to assess the spatial relationship between the targets. Or, it might be sufficient...non-specifically – try optimizing your blocking and washing conditions or consider using a different antibody...concentrations of all the different antibodies, blocking, washing, and sample preparation steps, in order to ensure...getting started with two or three targets or you’re pushing the limits like Addgene depositor Ronald Germain... Speranza, E., Chu, C. J., Gola, A., Thakur, N., Shih, R., Yao, L., Yaniv, Z. R., Beuschel, R. T., Kabat...

...located.  The export license, if granted, allows us to ship rabies plasmids and viral vectors containing genetic...control license, once approved, allows Addgene to ship plasmids or viral vectors containing sequences from... Once the application is approved, Addgene then ships the material following our normal process.   Due...have any questions about this or other Addgene shipping and ordering processes, don’t hesitate to contact...

... scientist. Leadership and management Take a lead from the amazing students at Washington University who...You are finishing your PhD or perhaps you have almost completed a postdoctoral position… or two. You ...in your department, create them and show your leadership skills too. Teach classes Go to workshops and...

...repository. 100,000 Plasmids Shipped! - By 2010, Addgene had hit our stride. We shipped our 100,000th plasmid... 10 years ago today. In that time, Addgene has shipped over 350,000 individual plasmids to 5,000 different...cells into pluripotent stem cells. Plasmids from Shinya Yamanaka's lab and James Thomson's lab were the...

...https://doi.org/10.1126/science.aav7271 Yoshimi, K., & Mashimo, T. (2022b). Genome editing technology ..., Demircioglu, F. E., Moeller, L., Kocalar, S., Oshiro, R., Makarova, K. S., Macrae, R. K., Koonin, E....j.mib.2017.05.008 Makarova, K. S., Wolf, Y. I., Alkhnbashi, O. S., Costa, F., Shah, S. A., Saunders, S. ... S., Wolf, Y. I., Iranzo, J., Shmakov, S. A., Alkhnbashi, O. S., Brouns, S. J. J., Charpentier, E., Cheng...

...absorbed. This phenomenon is known as the Stokes Shift, or the difference in wavelength between peak excitation...background signals when used with the same protein (Hayashi-Takanaka et al., 2014). Other dyes may prove challenging...accurate and reliable imaging and assay results (Hayashi-Takanaka et al., 2014)​​. Conjugates with lower...for intracellular fluorescence live imaging ​​(Hayashi-Takanaka et al., 2014). Dyes can also vary in their...), 987–994. https://doi.org/10.1038/nmeth.4403 Hayashi-Takanaka, Y., Stasevich, T. J., Kurumizaka, H.,...

..., hypercompetition is corroding values, we are shifting away from PI driven basic research, there is resistance...be radically overhauled.  Finally, the science publishing system needs to be changed.  Dr. Bourne charged...applause when he talked about how he boycotts publishing in all but open access journals.  Dr. David Glass...systemic flaws” by Bruce Alberts, Marc W. Kirschner, Shirley Tilghmanc and Harold Varmus     ...

... Their cooler boxes come with a prepaid return shipping label. To return the box, simply peel your address...details on what they accept and to print a free shipping label. Recycle nitrile gloves Terracycle is a .... They’re really easy to spot and even easier to ship back. Be that person! If you use serological pipettes...in your tissue culture room for the wrappers and ship them back. Start a glove recycling program at ...