We narrowed to 583 results for: BASE;

...allowing for in vivo biotinylation through a plasmid-based biotin ligase. Biotinylated dCas9, with bound DNA...genomic region is another example of the many tools based on targeting of a genomic locus through a specific...

...arises here. Notwithstanding access restrictions based on permissions, however, in some cases it would ...provides the RSpace electronic lab notebook. Rory is based in Cambridge, Massachusetts, and feels privileged...

...additionally allow for visual selection of transformants based on expression of RFP in the seed (Emami, Yee and...inflorescence (the flowering portion of the plant) near the base of the stem. This buys you a few days until new ...

...less photostable than GFP. In addition, antibody-based amplification of their signals is weak and increases...specific antibody binding sites and primary antibody-based detection, smFPs create much brighter signals for...

...genome and bind target DNA sequences using RNA-DNA base pairing. Separately, TnsABC forms a transposase ...tools such as Cas9-recombineering, CRISPRa/i, and base editing. Leveraging a unique marriage between distinct...

...demand model with weekend and holiday effects. Based on these observations, we considered the following...enable next-generation therapeutics. Prime editing, base editing, PACE, and DNA-templated synthesis are four...

...1990s, researchers developed viral vector systems based on retroviruses like Moloney murine leukemia virus...actively dividing cells. Another type of vector, based on adenovirus, could infect non-dividing cells, ...

...consistent development and improvement of CRISPR-based technologies over the past decade. CRISPR screens... fact supported by the wide array of lentiviral-based CRISPR libraries available at Addgene. The large...

...determined using a chromogenic endotoxin detection assay based on the amebocyte lysate method. Purity Purity of...Schematic of the barcode matching system and PCR-based confirmation of transfer plasmids used in Addgene...

...extrapolate the concentration of the unknown sample based on its measured response. An example of this is ..., the more accurate the curve. Though it varies based on the assay, the ideal %CV is less than 15%. You...

...Addgene blog Introduction to Antibodies Plasmid-based Recombinant Antibodies Polyclonal Antibodies Resources... Shokri A, Chotteau V. Antibody capture process based on magnetic beads from very high cell density suspension...

... Other fields, often highly technical or skills-based ones, may not use them very often. Similarly, entry-level...or sharing relocation plans. They can be skills-based: if you're switching fields, you can use your cover...

...conformational changes, cleavage activity using FRET-based biosensors, and interactions between DNA and proteins...limited to a single measurement. This method is based on the fact that the donor is quenched when FRET...

...response elements). Promoters are about 100 to 1000 base pairs long and found upstream of their target genes...Promoter specificity Aside from choosing a promoter based on type of RNA transcript, you will also need to...

...plasmids she can use as positive controls in PCR-based cervical cancer screens. This third grant represents...

...Tool for Gene Expression in Mammals Recombinase-based State Machines Enable Order-dependent Logic in vivo...

...References 1.Tang, Jonathan C.Y., et al. “A nanobody-based system using fluorescent proteins as scaffolds for...

...vectors are also likely to work in animal embryos based on earlier evidence showing that a CRISPR/Cas9 plasmid...

...details. Once you know the backbone a plasmid is based on, you can usually derive: a) the bacterial antibiotic...commercially available empty backbones, visit Vector Database. Step 2: Insert name  Include information about...

...cells. A major bottleneck, however, to these IP-based assays is to find (or generate in-house) a pre-validated...develop a hassle-free, highly efficient Cas9 protein-based method for epitope tagging in mammalian stem cells...will have to design your crRNAs using other web-based design tools (for C-terminus tagging, pick a crRNA...

... PAM requirements and editing abilities, such as base editing, nicking, cleaving, etc. The possibilities...toolkit to facilitate other lentivirus- and AAV-based studies. Unified MoClo Systems While many MoClo ...acssynbio.9b00322. Kim, Matthew S., et al. Degron-Based bioPROTACs for Controlling Signaling in CAR T Cells...

... the expertise he’s developed as well as CRISPR-based gene repression tools to find and test genes that...

...team creates stabs for each plasmid being shipped based on the information in our lab software, labeling...

...factor,” a measure that dates back to 1975 and is based on the average number of citations for recent articles...

...to understand. Classes are based on the main Cas effector; types are based on main function and composition...composition; subtypes and variants are typically based on species of origin. There are currently two classes...

...get its name? Often times, a gene gets its name based on what happens to an organism when a gene/protein.... Scientists reconstructed a transposase system based on the naturally mutated and non-functional transposase...

...a quick chat can be rather tricky (think people based in different countries). Here social media is a ...References 1. Sinche, Melanie, et al. "An evidence-based evaluation of transferrable skills and job satisfaction...

...nucleotide level but share an overall structure of 13 base pair palindromic repeats separated by an 8 bp asymmetric...2010. “An Improved FLP Deleter Mouse in C57Bl/6 Based on FLPo Recombinase.” Genesis 48(8): 512–20. PubMed...

...results (Sinnett et al., 2017; Powers et al., 2019). Based on the work of the consortium, two gene therapy ...guide RNA and a hyperactive version of the human RNA base editing enzyme (“Editase”), ADAR2, into the hippocampus...iPSCs, MECP2 animal models, tissue banks, Rett databases, and other helpful tools.  References: Amir ...

...point on the axis determined by the researcher, based on their controls. You can usually tell where this...Addgene blog Conventional vs. Flow Cytometry Plasmid-based Recombinant Monoclonal Antibodies Introduction to...

...regulators. The genome of E. coli is about 4.6 million base pairs long and contains a single origin of replication...into a “pseudoknot” held together by intramolecular base pairs for the protein to be translated. An antisense...

...lab has developed a multiplexing approach to Cas9-based pooled CRISPR screens called CROPseq-multi (Walton...immunogenicity typically associated with viral vector-based therapies. REVeRT supports advanced gene expression...negative-going sensors (with fluorescent signal at baseline voltage), the investigators found that administering...

...environment. The “how many?” question lies at the base of all these questions as there is not particularly...for their buck - make them demand more services based on what they're spending. Increase the number of...

...off-target effect. The double nickase system is based on the Cas9 D10A nickase described in Figure 4 of...express without the G. Because the G is only one base, the Zhang lab usually adds it when they order the...oligo from IDT (non-PAGE purified oligo is fine based on new data). For general transfection, you could...

...Scientific Officer “Choose your PhD lab carefully. Don’t base it solely on the project. Assess whether or not ...

..., and now researchers have reported a mechanism based on studies of transgenic mice that helps to explain...

...organism. We have designed a Golden Gate system based on the daily needs of an advanced molecular plant...

...MIT, the use of customizable DNA-binding domains based on transcription-activator-like effectors (TALEs...

...popular counterselection vector is the pDS132 sacB-based allelic exchange vector. This vector contains sacB...expression in Escherichia coli using a rhamnose-based tightly regulated and tunable promoter system." ...

...development. Stochastic multicolor labeling systems, many based on Brainbow, now exist for a variety of species,...Labeling Drosophila neurons with dBrainbow and Flybow Based on the Brainbow 1.0 system, Drosophila Brainbow ...