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  3. An Addgene Summer Internship

    Type
    Blog Post
    Published
    Aug. 8, 2024, 7:22 p.m.
    ...should be a max of 500 bp (preferably). I compiled a list of three options from the Addgene catalog. These...osa-miR528. My manager, Katherine N., reviewed the list and followed up with her manager. They looked at...

  6. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    Published
    June 5, 2017, 3:19 p.m.
    ...information.” Addgene is here to help scientists accomplish their goals. Many nonprofits must spend a lot...Seeding Labs Addgene Supports Rare Disease Research Listen to Our Interview with Addgene Board Member, Connie...

  8. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    Published
    Dec. 9, 2014, 2:22 p.m.
    ...”. I have added a few comments of my own to this list. Need feedback on their work but prefer to be approached...Addgene Blog to follow the entire series as it is published. Good introductory reference books on managing...

  9. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ...backbones that have been designed, tested, and published by academic scientists. To help you find the vector...Molecular Cloning Guide & Other Plasmid Protocols List of common epitope tags ...

  10. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    Published
    March 28, 2022, 4:30 p.m.
    ...Bandrowski et al., 2016). It’s also been well established that recombinant antibodies are excellent for...Bechtold H, Dumitras C, Gong B, Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS (2019) A ...

  12. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    Published
    Aug. 11, 2020, 1:15 p.m.
    ...routine plasmid propagation, with complete ITR loss abolishing AAV packaging. To prevent ITR loss, it’s best...://doi.org/10.3389/fnana.2019.00093 Wilmott P, Lisowski L, Alexander IE, Logan GJ (2019) A User’s Guide...

  14. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    Published
    March 9, 2017, 3:40 p.m.
    ...the diverse options in nonprofit science. No one list can ever encompass them all. We can’t designate ... like you What is different about academia? Publishing in peer reviewed journals is absolutely necessary...

  15. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    Published
    June 9, 2022, 1:15 p.m.
    ...are interested in breeding dogs who also enjoy listening to podcasts A series of Tweets breaking down...likely to closely read an email sent out to a large listserv or one sent to you and a few other people on your...

  16. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    Published
    Oct. 20, 2020, 1:15 p.m.
    ... need, and most of us could use more mentors to listen, guide and support us during training and throughout...your head.  Here are some sites that have compiled lists of grants: Science: Where to Search for Funding...

  17. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    ...for being a part of our community, we’ve curated a list of our 10 top-viewed posts from 2015 and encourage...of our career posts has made it to the top of the list this year. While we love academic research, we hope...

  18. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    Published
    April 7, 2015, 4:21 p.m.
    ...cellular behaviors, such as differentiation or metabolism. Our attempts to manipulate these cellular behaviors...promise to be host-independent, beyond the oft published metabolic engineering realm of S. cerevisiae and...

  19. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    Published
    May 6, 2014, 1:07 p.m.
    ...sensitivities can now be applied to dozens of other published FRET-based sensors. For example, his team is now... improve upon an indicator they developed and published in 2010 for visualizing the transcription factor...

  21. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    Published
    Aug. 22, 2023, 1 p.m.
    ...researchers are open to sharing knowledge and establishing collaborations, so it doesn’t hurt to reach ...will need, any required training, and a priority list of experiments you hope to complete. Share this ...

  22. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ...enzymes, the app is quite helpful and provides a list of directions including which buffers to use and... I have found myself wishing that it had a codon list for each amino acid, but, overall AMINO can be beneficial...

  24. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    Published
    Aug. 13, 2015, 2:30 p.m.
    ...further introduce him to the Addgene community. Listen to the interview here! Doench majored in history... Edward B. Page is a Digital Media Production Specialist at Addgene. He is the audio engineer and producer...

  25. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    Published
    Oct. 9, 2014, 3 p.m.
    ...available. With new fluorescent proteins being published every month, deciding on the best protein for ...common use (particularly not the most recently published ones), they can be a good starting point. In many...

  26. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ...the mid-aughts, the first yeast GCE paper was published, followed soon by GCE done in mammalian cells ...community to fulfill their mission. While they’ve shortlisted several protocols, including their phosphoserine...

  27. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    Published
    March 21, 2023, 1:15 p.m.
    ...infect both non-dividing and dividing cells. They accomplish this through nuclear localization signals which...on the Addgene Blog Learn More about Lentivirus Listen to Connie Cepko Discuss Gene Therapy with AAV Figure...

  28. Antibodies 101: Isotypes

    Type
    Blog Post
    Published
    Oct. 19, 2021, 1:15 p.m.
    ...monoclonal, polyclonal, scFvs, nanobodies, and the list goes on. But have you heard of an antibody isotype...cytometry, immunohistochemistry, immunocytochemistry, ELISAs, and Western blotting.  The five isotypes of antibodies...

  32. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...lentiCRISPR v2.(If you’ve peeked through the rest of the list at this point, you may have noticed that many other...lentiviral) or pUMVC (MuLV retroviral). Fun fact: published in 2003, this plasmid predates Addgene’s founding...

  34. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ...plasmid carrying the required gene.  The table below lists some of the most commonly used selection markers...said element. This link provides a more extensive list of yeast auxotrophic markers and includes the associated...

  35. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    Published
    Feb. 18, 2014, 4:22 p.m.
    ...group of mentees to get more “bang for the buck”. Listen to our podcast interview with Harvard Medical School... the internet, but start by downloading my handy list of suggestions.  Get started. Have regular meetings...

  36. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...affinity reagents.    Types of Affinity Reagents  (Listed in no particular order.) Fab fragments are created...distinction of being the only affinity reagent on this list derived from nature. Single-domain antibodies are...

  37. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ...cytometry’s standard approach is the direct method. ELISAs, immunohistochemistry, and immunocytochemistry ...Introduction to Immunofluorescence Antibodies 101: ELISAs Antibodies 101: Introduction to Antibodies Resources...

  38. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...was frustrated by the difficulty of accessing published plasmids from other scientists. She, Kenneth Fan...others could request from — Addgene!  Addgene was established as an e-commerce company, which helped it stay...

  39. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    Published
    April 10, 2018, 12:56 p.m.
    ...tissues. Although antibody production is well established, it’s also a process that’s difficult for individual...their RANbodies, Yamagata et al used previously published nanobody sequences that targeted the following...

  40. Developing Lab Management Software for Biology

    Type
    Blog Post
    Published
    Aug. 25, 2016, 2:30 p.m.
    ...point, decide early on what to do in a namespace collision. It may even be worth it to have extra columns...solving, but consistency is key. Some namespace collisions you may encounter: vector, sequence, insert, ...
Showing: 321 - 360 of 611 results