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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 573 results for: BASE

Showing: 401 - 450 of 573 results

  2. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ...Fly vectors The vast majority of fly vectors are based on p-element, random or targeted, integration into...

  3. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ...synthetic compounds. Antibiotics can be categorized based on whether they directly kill bacteria (bactericidal...

  4. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ... a single allele in a human line, using methods based on homologous recombination (Rago et al., 2007)....opportunities, it’s an exciting time to be in the lab. Based on the power of available techniques and how quick...nuclease cleaves double stranded DNA (dsDNA) at basepair mismatches. To use this tool to detect a BRAF ...strand from the control cells, which should contain basepair mismatches at the site of the edit. Using Surveyor...

  5. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    ...against unnecessary clinic regulations, evidence-based research is vital in combating misinformation and...83.7430°W – Alexandra Greenbaum Science and evidence-based policy and legislation hit home hard in Michigan...

  7. Plasmids 101: Origin of Replication

    Type
    Blog Post
    Published
    Nov. 11, 2020, 2:23 p.m.
    ...generally high in As and Ts. Why, you ask? Well, A-T base pairs are held together with two hydrogen bonds ...

  9. An Addgene Summer Internship

    Type
    Blog Post
    Published
    Aug. 8, 2024, 7:22 p.m.
    ...expected something wrong. My capstone project was based on plasmid cloning and I, the researcher, got to...

  10. The MTA at Addgene

    Type
    Blog Post
    Published
    July 23, 2024, 2:17 p.m.
    ...be distributed for research use only to industry-based scientists. Some organizations choose to share their...

  11. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    Published
    April 25, 2023, 1:15 p.m.
    ... antibodies. Recombinant antibodies are plasmid-based and are created synthetically or derived from hybridoma...

  26. Components of CRISPR/Cas9

    Type
    Blog Post
    Published
    Feb. 2, 2016, 5 p.m.
    ... sequence contained within the guide RNA and a 3-base pair sequence known as the Protospacer Adjacent ...

  27. Finding and Joining Your Dream Lab

    Type
    Blog Post
    Published
    April 8, 2014, 2:50 p.m.
    ...interests.” George Church selects new hires initially based on those who notice and embrace the “odd and extreme...

  29. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ... Knockout cell line approach Genetic knockout-based validation can conclusively prove the ability of... through its ability to separate the cell lines based on expression of the target. In this case, you could...

  32. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...Blast Most of Addgene’s lentiviral inventory is based on CRISPR tools, though we do provide a few non-...

  38. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ...Delivery: Potential Applications for Gene and Cell-Based Therapies in the New Era of Personalized Medicine...

  39. Hot Plasmids - May 2022

    Type
    Blog Post
    Published
    May 26, 2022, 1:15 p.m.
    ... Fig. 3: Development of a cleavage-free dCas9-based gene editor using microbial SSAPs. Image from Wang... system has a much lower error rate. For a one kilobase insertion, the researchers reported an editing...AAV system. dCas9-SSAP will make the editing of kilobase-scaled sequences more efficient and less risky...

  42. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ... to identify each protein of interest, this gel-based method identifies proteins solely by weight and ...

  43. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...about the advantages of using recombinant plasmid-based antibodies Resources on Addgene.org Discover Addgene...

  44. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    Published
    Feb. 2, 2021, 2:15 p.m.
    ...statement. Make certain to choose your recommenders based on your relationships to the person as well as their...resources, we created STEAMid, for a nationwide database of  STEAM internships for high school, college...

  46. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ...reliable sequence data back for the first 50-100 base pairs of your sequencing read. You can expect to...mammalian selection markers. You don’t need a fancy database to organize your own plasmid collection, although...help of DNA-editing software which integrates our database of over 2000 primers. Two 96-well PCR plates are...Finally, you will want to have a well-organized database of expected sequences, either for the inserts ...Get Plasmid Backbone Information from Our Vector Database  ...

  48. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ... CRISPR guides that flank regions of hundreds of base pairs or more can simultaneously introduce a pair...

  49. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ...marker help synthesize? Counterselection? (growth-based positive selection for the loss of the marker gene...
Showing: 401 - 450 of 573 results