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Addgene
Showing: 401 - 450 of 510 results

  1. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...-transfect cell types ranging from primary to embryonic stem cells. Viral integration of a construct is...

  2. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ... yet low-pressure teaching opportunity for lab members or students new to protein expression and purification...

  4. Antibodies 101: Isotypes

    Type
    Blog Post
    Published
    Oct. 19, 2021, 1:15 p.m.
    ...tears, saliva, mucus) where it protects mucosal membranes. This isotype is most commonly a dimer with a ...

  9. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    Published
    June 29, 2017, 1:06 p.m.
    ...colleagues, or explain plasmid features to a lab member when you’re not at your computer. Maps, sequences...

  10. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Published
    May 25, 2017, 2:30 p.m.
    ...publication in 2012, the Nagai laboratory has assembled a collection of multicolored nano-lanterns for...

  13. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ... works in Dale’s lab.        Dale Ramsden is a member of the curriculum in Genetics and Molecular Biology...

  15. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...labhold” name. Our first plasmid request came on September 8, 2004, for pcDNA-p160MBP, while the first request...

  17. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ...journal and share it more widely!  Not everyone embraces preprints as part of their scientific communication...

  18. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...expression for several years (Wonjo et al., 2013). Remember that AAVs do not integrate into the host genome...

  19. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...through mutation, allowing diabodies to be used for assembling protein nanostructures. Diabodies readily co-...

  23. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...GFP-dCas9 and the gRNA scaffold to enhance complex assembly, thereby decreasing the amount of background fluorescence...

  35. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ... in clinical trials includes Cas3 (the shredder member of the Cas family) as part of a cocktail therapy...

  36. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ...biosafety cabinet. When decontaminating liquid waste, remember that the percentage indicates the proportion of...

  38. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ...’ve since returned to pgEd as a full-time staff member for the past three years. pgEd is a small team ...

  42. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...about what reviewers in your area are looking for. Remember though, you need to leave them time to read it...

  43. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ... money on a potentially faulty product. Please remember that the person at the other end of that email...

  44. Common Injection Routes in Mice

    Type
    Blog Post
    Published
    Jan. 22, 2025, 9:42 p.m.
    ...When considering what method you’d like to use, remember that the final decision will likely come down ...

  46. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...this surface”….where the complex is docked on the membrane of the endoplasmic reticulum. And graduate students...

  47. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...mapping functional domains of Drosophila hsp 70. Embo J 3:3087–3093. https://doi.org/10.1002/j.1460-2075.1984...

  48. Transferable Skills: Negotiation

    Type
    Blog Post
    Published
    March 3, 2022, 2 p.m.
    ...colleagues and supervisors and lead to you being remembered as a person who tries to find solutions that ...

  49. Pooled Library Amplifications

    Type
    Blog Post
    Published
    May 30, 2024, 1:30 p.m.
    ... protocols attached.  Final step: validation  Remember, there is no “one-size-fits-all” protocol! Each...

  50. Antibodies 101: Flow Compensation

    Type
    Blog Post
    Published
    May 16, 2024, 1:15 p.m.
    ...But we hope this guide can help you get started. Remember, you can always change your compensation values...
Showing: 401 - 450 of 510 results