We narrowed to 481 results for: mme
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TypeBlog PostPublishedFeb. 7, 2019, 2:16 p.m....do the same for you. Send us your questions or comments to [email protected]. We're eager to ...
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3D Printing Meets CRISPR Cas9
TypeBlog PostPublishedOct. 17, 2017, 12:55 p.m....family-run business – 3D Molecular Designs – to commercialize some of the best instructional materials that... -
Truncated gRNAs for Regulating Gene Expression
TypeBlog PostPublishedJan. 10, 2017, 3:37 p.m....making targeted modifications to DNA, Cas9 can be reprogrammed to serve as a regulator of gene expression. ... -
Experimenting with New Careers while in Grad School
TypeBlog PostPublishedFeb. 25, 2016, 3:30 p.m....organization. My short experience with my blog helped immensely when CINSO started brainstorming the resources... -
Genome-wide Screening Using CRISPR
TypeBlog PostPublishedAug. 18, 2015, 2:30 p.m....compared to the rest of the genome and located immediately 5’ to a protospacer adjacent motif (PAM) sequence... -
Plasmids 101: Multicistronic Vectors
TypeBlog PostPublishedSept. 9, 2014, 8:20 p.m.... a backbone as a single unit. Although it is recommended to use the 2A peptides instead of an IRES when... -
Which Fluorescent Protein Should I Use?
TypeBlog PostPublishedMay 20, 2014, 2:06 p.m....function of the FP-fusion protein. Therefore, it is recommended to use monomeric FPs (usually denoted by a “m... -
Great Results Start with Great Standard Curves
TypeBlog PostPublishedAug. 15, 2024, 1:15 p.m....involving sigmoidal curves, manufacturers often recommend using advanced graphing software designed to plot... -
An Introduction to Adenovirus
TypeBlog PostPublishedOct. 3, 2023, 1:15 p.m.... humans (and no vaccines are available on the commercial market). Although host-virus interactions likely... -
Course-Based Undergraduate Research Experiences (CUREs)
TypeBlog PostPublishedFeb. 9, 2023, 2:15 p.m....exciting opportunity for your classroom, I highly recommend you try implementing one. It's likely only a matter... -
CRISPR 101: Making a Knock-In Cell Line
TypeBlog PostPublishedDec. 22, 2022, 2:15 p.m....and ovarian lines commonly used in lab. It is recommended you check your cell lines for HR-related mutations... -
AAVs in Retinal Gene Therapy
TypeBlog PostPublishedJuly 31, 2018, 12:55 p.m....it at the time, but gene therapy had fallen - plummeted really - from grace 2 years prior with the death... -
SciComm: Taking Science to Elementary and Middle Schools
TypeBlog PostPublishedJuly 9, 2019, 12:52 p.m.... to answer some of their great questions, was immensely gratifying. I came out of the experience with ... -
Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!
TypeBlog PostPublishedDec. 3, 2019, 11:38 a.m....recombinant monoclonal antibody plasmids from the James Trimmer Lab published in eLife. Addgene is currently distributing... -
INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons
TypeBlog PostPublishedDec. 7, 2020, 2:53 p.m....Makarova KS, Koonin EV, Zhang F (2020) Response to Comment on “RNA-guided DNA insertion with CRISPR-associated... -
The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data
TypeBlog PostPublishedJuly 16, 2020, 1:15 p.m....potential instabilities in the China data, so we recommend interpreting the model results for China with ... -
Negative Can Be Positive: Open AAV Data with Addgene
TypeBlog PostPublishedSept. 6, 2022, 1:15 p.m.... J., Bahr-Davidson, J., Smith, P. H., Zhen, Z., Sommer, J. M., Pierce, G. F., & Qu, G. (2005). Identification... -
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics
TypeBlog PostPublishedAug. 9, 2016, 2:30 p.m....HIV. By design, our lentivirii are genetically programmed to CRISPR out the enhancers we identified in ... -
Lentiviral Vector Uses and Overview
TypeBlog PostPublishedMay 19, 2016, 2:30 p.m....experiment. Let us know how you use lentivirus in the comments section below! References 1. In vivo gene delivery... -
CRISPR Antimicrobials
TypeBlog PostPublishedMay 3, 2016, 2:30 p.m....Udi Qimron. “Temperate and lytic bacteriophages programmed to sensitize and kill antibiotic-resistant bacteria... -
Tips for Screening with Yeast Two Hybrid Systems
TypeBlog PostPublishedOct. 22, 2015, 2:30 p.m.... D., Revuelta, J.L., Boles, E., André, D., and Frommer, W.B. K+ channel interactions detected by a genetic... -
Plasmids 101: Protein tags
TypeBlog PostPublishedDec. 11, 2014, 4:26 p.m....developed (and just because your protein has a commercially available antibody, that doesn’t mean it’s a... -
Your Lentiviral Plasmid FAQs Answered
TypeBlog PostPublishedApril 23, 2014, 1:08 p.m....other questions? Feel free to post them in the comments and we would be happy to answer them! Note: A... -
Twenty Years of Sharing: Expanding Accessibility
TypeBlog PostPublishedNov. 21, 2024, 10:47 p.m.... media, on collaborators’ websites, or as a recommendation in a Reddit thread crowdsourcing experimental... -
Twenty Years of Sharing: Addgene's Viral Vector Service
TypeBlog PostPublishedJuly 30, 2024, 1:15 p.m....Suggest a viral vector for our service; leave a comment; or take our AAV survey. Learn more about GCaMPs... -
Antibodies 101: The Four ELISAs and When to Use Them
TypeBlog PostPublishedJuly 25, 2024, 1:15 p.m....Users may also find it difficult to source a commercially available and appropriately conjugated antibody... -
Hot Plasmids Spring 2024
TypeBlog PostPublishedMay 23, 2024, 1:15 p.m....component for viral genome packaging. Addgene recommends both for use in western blots (Figure 3). ... -
Transferable Skills Guide: External Collaborations
TypeBlog PostPublishedApril 19, 2018, 1:25 p.m....people on twitter and maintain a connection by commenting and sharing. LinkedIn or ResearchGate allow you... -
Transferable Skills Guide: Creativity
TypeBlog PostPublishedJan. 18, 2022, 2:15 p.m....person speaks up after a group consensus and is immediately followed by several other people with their own... -
CRISPR 101: Targeting RNA with Cas13a (C2c2)
TypeBlog PostPublishedSept. 21, 2017, 2:07 p.m.... non-specific cleavage is thought to activate programmed cell death or a dormant state for bacterial cells... -
Fluorescent Proteins 101: Aptamer Fluorophores
TypeBlog PostPublishedApril 11, 2017, 2:30 p.m....done largely in vitro; Easy and reproducible commercial synthesis (those of us who have used antibodies... -
Save Time with Transient Plant Leaf Transformations
TypeBlog PostPublishedJuly 25, 2019, 12:03 p.m....free to add further tips and experiences in the comments below. Many thanks to our guest blogger Samuel... -
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone
TypeBlog PostPublishedFeb. 18, 2020, 2:15 p.m....Gunn and Dr. Denise M. Akob for reviewing and commenting. Binning Singletons is especially grateful to... -
The PAM Requirement and Expanding CRISPR Beyond SpCas9
TypeBlog PostPublishedAug. 20, 2020, 2:30 p.m....experiment, check out our CRISPR guide or leave a comment below. Note: Jennifer Tsang and Mary Gearing ... -
Lab to Office Culture Shock
TypeBlog PostPublishedFeb. 5, 2019, 1:04 p.m....different. Some of these changes you may enjoy immediately and some of them may just take some getting used... -
CRISPR-mediated Plant Base Editors
TypeBlog PostPublishedJan. 3, 2019, 1:35 p.m....the PAM sequence. Avoid targeting a ‘C’ placed immediately after a ‘G’ base because CBE is inefficient in... -
Bacterial Toxin-antitoxin Systems as Molecular Biology Tools
TypeBlog PostPublishedNov. 1, 2018, 12:35 p.m....corrected] 3', 5'-bispyrophosphate: a model for programmed bacterial cell death." Proceedings of the National... -
Career Insights: Technical Support Specialist
TypeBlog PostPublishedNov. 9, 2017, 2 p.m....an academic lab to industry without being too commercial or “sales-y”. The work includes reviewing customer... -
Antibodies 101: Normalization and Loading Controls for Western Blots
TypeBlog PostPublishedApril 8, 2025, 1:15 p.m....should validate it for your particular setup. We recommend starting by searching the literature to see what... -
New Optogenetic Tools for Cytoskeleton and Membrane Control
TypeBlog PostPublishedSept. 28, 2023, 1:15 p.m....10.1016/j.ceb.2015.08.005. Tei, R., Bagde, S. R., Fromme, J. C., & Baskin, J. M. (2023). Activity-based ... -
Adeno-associated Viruses (AAVs) for Genome Editing
TypeBlog PostPublishedMarch 27, 2018, 1:32 p.m....tagging vectors for your specific gene, the lab recommends designing homology arms with the modification... -
Transferable Skills Guide: Conflict Resolution
TypeBlog PostPublishedJan. 4, 2022, 2:15 p.m....also be good. In science, a reviewer’s critical comments on your manuscript is a form of (hopefully constructive... -
Antibodies 101: Single Chain Fragment Variables (scFvs)
TypeBlog PostPublishedJune 3, 2021, 1:15 p.m.... antibodies for neuroscience research from the Trimmer lab Check out Addgene's latest project to develop... -
Filming Science Videos in the Age of Social Distancing
TypeBlog PostPublishedMay 20, 2021, 1:15 p.m....performance. By this point, towards the end of the summer, things were getting better and the office had ... -
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells
TypeBlog PostPublishedSept. 5, 2017, 1:58 p.m....tolerate a mismatch in the binding sequence, we recommend changing several bases as close to the PAM as ... -
Harnessing Bacterial Toxins for Allelic Exchange
TypeBlog PostPublishedAug. 15, 2019, 12:30 p.m.... cut vector with excellent efficiency using a commercial isothermal assembly master mix. Introduction ... -
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior
TypeBlog PostPublishedMarch 5, 2019, 1:08 p.m....homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells." Cell 143.1 (2010)... -
CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9
TypeBlog PostPublishedOct. 11, 2016, 2:30 p.m....with all plasmids in the repository, we highly recommend reading the associated publications or protocols... -
Multiple Plasmids at a Low Price: Kits from Addgene
TypeBlog PostPublishedSept. 14, 2016, 2:30 p.m.... have any questions, please write to us in the comments section. FX cloning kit This kit is designed... -
Using Phosphoserine to Study Protein Phosphorylation
TypeBlog PostPublishedJune 23, 2016, 2:30 p.m....extensively worked with and optimized Sep-OTSλ and can recommend its use (6). 2. C321, a specialized bacterial...