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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 574 results for: BASE;

Showing: 161 - 180 of 574 results

  1. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...developed sequence-specific, antimicrobial, plasmid-based, CRISPR systems (Find the Citorik Plasmids here)...encoded quinolone resistance, mediated by a single base pair change in DNA gyrase, CRISPR phagemids were...crRNAs) targeting either genomic (blue) or plasmid-based sequences (red). Treating a mixed population, represented... al. recently engineered synthetic phages, each based on the well-studied T7 phage, to target various ...

  2. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...Though there are a number of chemical and peptide-based proteosome inhibitors, glutathione S-transferase...DYKDDDK 1 Affinity and Purification Good for antibody-based purification; has inherent enterokinase cleavage...residues improves solubility; good for antibody-based detection SUMO ~100 amino acid protein  12 Stability...1.4 Affinity and Purification Good for antibody-based purification Combo and cleavage tags Frequently...

  3. PEI Calculator for Planning AAV Packaging Transfections

    Type
    Blog Post
    Published
    Jan. 23, 2018, 2:04 p.m.
    ...names of the plasmids you plan to use Enter the base pair size of those plasmids Enter plasmid concentration...plasmid needed per dish or stack. Calculations are based on using 28 μg of total plasmid (Transfer, helper...

  4. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...postsynaptic receptors and transporters. The intensity-based Glutamate-Sensing Fluorescent Reporter (iGluSnFR...The Abudayyeh-Gootenberg Lab developed this tool based on several new site-specific retrotransposon elements...can make a wide variety of genomic edits — single-base changes, small deletions, and large insertions up...insertion is scarless, unlike previous integrase-based approaches. Find STITCHR plasmids here! Fell, C...primates, and human tissue. Figure 6: Enhancer-based targeting of PV interneurons in mice. A) AAV expression...

  5. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ...science better when we share? NanoLuc® Luciferase-based tools are fantastic examples of technologies that...Nano-Lanterns to other cellular proteins. BRET-based biosensors utilizing NanoLuc® luciferase Many intracellular...powerful the FRET signal. In well-designed FRET-based biosensors, the intensity of the FRET signal can...Schmid, J.L., et al. (2016) CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent...) Coupling optogenetic stimulation with NanoLuc-based luminescence (BRET) Ca++ sensing. Nature Comm. 7...

  6. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...glycosyl groups, and other modifications to their bases in order to protect their DNA from host cell nucleases...proteins in the (all-too-common) event that homology-based searches return no candidates. Our strategy: Express...in the presence of certain modifications to DNA bases.  Ligate and transform your resultant plasmid libraries...Adapting drug approval pathways for bacteriophage-based therapeutics."Frontiers in Microbiology 7 (2016)...Raleigh. "Biosynthesis and Function of Modified Bases in Bacteria and Their Viruses." Chemical Reviews...

  7. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ...you through both experimental and primer design based on fragment sequences and the polymerase used in...joining of multiple mutations spread over thousands of base pairs. Cloning sgRNAs Generating constructs to produce...Cloning Gibson Assembly and Other Long-Homology Based Cloning Methods Screen Plasmids by Colony PCR Addtional...

  8. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    ...plasmids and those without, we adjust our estimates based on the journal that published the articles.  That...Addgene plasmids as if they comprised 10 percent. Based on this new re-weighting, we can compare citations...Addgene is achieving its goal of making plasmid-based science more accessible…and accruing more citations..., we went to one of the largest bibliographic databases available, Web of Science. For each publication...number of citations the paper receives. From the database we pulled the journals from 2005 to 2015 in areas...

  9. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ...plasmids used to generate these antibodies were based off of a large collection of mouse monoclonal antibodies...Antibodies?  One of the benefits of using a plasmid based system for expression of R-mAbs is that it can easily...Additional Resources on the Addgene blog:  Plasmid-Based Recombinant Antibodies  Plasmids 101: Secondary ...

  10. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    Published
    Feb. 10, 2016, 3:30 p.m.
    ...genomic SDM relatively straightforward, but plasmid-based SDM has lagged behind. While commercial kits are...PCR is used to splice together two PCR amplicons based on sequence homology, and the subsequent product...primers also have some homology to each other; ~17 bases is optimal. After being treated with DpnI to digest...

  11. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    Published
    Sept. 8, 2015, 2:30 p.m.
    ...limited to neuroscience. In fact, precise light-based control of biological processes is highly useful...better fine-tune the level of output gene expression based on the input light intensity. In addition to these...strains of E. coli, as well as with other plasmid-based systems, which will enable the assembly of larger...

  12. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    Published
    Dec. 10, 2013, 3:13 p.m.
    ...a DNA-binding domain to recognize any desired 15 base pair sequence in the genome in rapid fashion. The...All you do to create a protein that binds those 15 base pairs is take four plasmids out of the library in...genome-editing system for generating human stem cell-based disease models." Cell Stem Cell. 12, 238-251 (7 ...

  13. Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More

    Type
    Blog Post
    Published
    Nov. 15, 2016, 3:30 p.m.
    ...and what work he's planning on doing with CRISPR-based gene drives focusing on safety and the ability to...Board with a particular interest in generating Cas9 based tools to enable facile control of DNA and RNA.   ...

  14. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ... knowledge base is going to necessarily need a diverse group of people to provide that base. Sometimes... Mission accomplished! One example of a mission-based team in academia would be your school’s post-doc...Let’s think about some of the advantages of team-based work: Nobody knows everything anymore John F. Kennedy... post, so here are just a few critical features based on my own experience: Leadership Few things are ...

  15. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...are functionally differentiated from one another. Based on their structural elements, oris interact with...proteins within different host organisms. pBBR1-based plasmids and plasmids in incompatibility groups ...campestris pv. malvacearum, Zymomonas mobilis pBBR1 based pBBR1  Gram-negative  Alcaligenes eutrophus...constitutive and IPTG-inducible integron promoter-based expression systems for Escherichia coli and Agrobacterium...

  16. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    Published
    Sept. 5, 2017, 1:58 p.m.
    ... et al) we were very excited to set up a CRISPR-based editing platform in our lab. Our goal was to use...binding sequence, we recommend changing several bases as close to the PAM as possible (see below to find...Ludwig-Maximilians-University Munich. His lab develops iPSC-based models of neurodegenerative and neurovascular diseases...of Alzheimer’s disease by developing novel iPSC-based model systems.     References 1. Cong, Le, et al...

  17. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    Published
    Nov. 15, 2017, 3 p.m.
    ...infectious titer of a viral vector stock can vary based on transduction target and can also be altered by...titer is typically calculated by two popular PCR-based methods: Quantitative PCR (qPCR) Digital droplet...not widely used today (Fagone et al., 2012). PCR-based methods are robust, easy, fast, and convenient. ...quantified with a different primer pair. Ct values vary based on the amount of starting material in the PCR because...

  18. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ...of the desired mutation (usually 1-3 mismatched bases) is sufficient for your primers to successfully ...a minor extension can usually ensure that the 3’-base(s) do not form secondary structures. The introduction...feature used in TA-cloning). Such non-complementary bases at the 3’-end interfere with plasmid reconstitution...et al. "Predicting DNA duplex stability from the base sequence." Proceedings of the National Academy of...

  19. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...the best possible results for any tissue culture-based experiment, labs should routinely test for mycoplasma...detector cell lines, ELISA, fluorometry, or PCR-based approaches to detect even low levels of contamination...detection, Uphoff et. al. outline a do it yourself PCR-based approach complete with freely available positive...concentration and titration of pseudotyped HIV-1-based lentiviral vectors."Nature protocols 4.4 (2009):...

  20. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ... decisions based on experimental timelines and specific aims. 2. Then make decisions based on financial...instructions on creating a budget, making decisions based on that budget, and negotiating in regards to the...will need, and how long it will take to publish based on these findings. Most often we suggest estimating...
Showing: 161 - 180 of 574 results