We narrowed to 208 results for: CIL
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TypeBlog PostPublishedJan. 10, 2017, 3:37 p.m....molecular biology and bioengineering, as it has facilitated the development of a simple and scalable means...
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PITChing MMEJ as an Alternative Route for Gene Editing
TypeBlog PostPublishedFeb. 23, 2016, 3:30 p.m....using much smaller regions of homology, which facilitates easier cloning. An overview of PITCh. ... -
Light Sheet Fluorescence Microscopy
TypeBlog PostPublishedJuly 16, 2015, 2:30 p.m....ubiquitin C promoter. Anatomical abbreviations: Gr= Gracile tract, Cu= Cuneate tract, dCST= dorsal cortico ... -
CRISPR 101: Ribonucleoprotein (RNP) Delivery
TypeBlog PostPublishedSept. 6, 2018, 12:02 p.m.... research has shown that a Cas9 protein from Geobacillus stearothermophilus is more stable in human plasma... -
MXS Chaining
TypeBlog PostPublishedFeb. 7, 2017, 3:30 p.m.... 3. The goal of high throughput cloning is to facilitate the construction of plasmids with many components... -
CRISPR 101: Making a Knock-In Cell Line
TypeBlog PostPublishedDec. 22, 2022, 2:15 p.m....carefully (Lin et al., eLife)! Use a Cas that facilitates HR What if promoting HR was as simple as swapping... -
A Guide to Starting Your Own Journal Club
TypeBlog PostPublishedFeb. 6, 2020, 3 p.m....level of critique and detail? Who is/are the facilitator(s) of the journal club? Where will the journal... -
CRISPR Antimicrobials
TypeBlog PostPublishedMay 3, 2016, 2:30 p.m....opportunistic infection like for organisms like C. difficile. In 2014, Citorik et al. and Bikard et al. developed... -
A Career in Grants: How To Become a Grant Professional
TypeBlog PostPublishedAug. 20, 2015, 2:30 p.m....national government offices, schools, healthcare facilities and with private businesses. How do you get the... -
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)
TypeBlog PostPublishedMay 15, 2014, 3:33 p.m....deficiencies found in the wildtype protein and facilitating its widespread use in research. Many other mutations... -
Negotiating Work and Life: How to Find the Joy
TypeBlog PostPublishedMarch 20, 2014, 3:04 p.m.... reminded her doctor that she was allergic to penicillin – I am much less worried knowing that she can... -
Hot Plasmids - August 2020
TypeBlog PostPublishedAug. 18, 2020, 1:15 p.m....expression. This includes a CRISPRi-based synthetic oscillator, bistable network, stripe pattern-forming incoherent... -
Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More
TypeBlog PostPublishedAug. 13, 2019, 12:58 p.m....model systems, and their resistance (so far) to facile CRISPR/Cas9-mediated insertion of foreign DNA. ... -
Bioinformatics at Addgene
TypeBlog PostPublishedJuly 14, 2022, 1:15 p.m....we have moved to full plasmid sequencing at our facility using Illumina MiSeq Next Generation Sequencing... -
AAV Titers: Where Do They Come From And What Do They Mean?
TypeBlog PostPublishedNov. 15, 2017, 3 p.m.... Addgene and other viral vector manufacturing facilities report the physical titer of a viral solution... -
CRISPR-based Genome Editing Systems For Editing in E. coli
TypeBlog PostPublishedApril 13, 2021, 1:15 p.m....strain library with different gene dosages to facilitate swift optimization of strains. Multiple induction... -
5 Reasons to Use Reddit for Science Communication
TypeBlog PostPublishedOct. 20, 2016, 2:30 p.m....r/Science has a great team of moderators who facilitated scheduling, filtered out inflammatory questions... -
Important Considerations in Optogenetics Behavioral Experiments
TypeBlog PostPublishedOct. 1, 2015, 2:30 p.m....transmission from the amygdala to nucleus accumbens facilitates reward seeking." Nature 475.7356 (2011): 377-... -
Plasmids 101: E. coli Strains for Protein Expression
TypeBlog PostPublishedFeb. 10, 2015, 3:06 p.m....thioredoxin reductase and glutathione reductase to faciliate proper folding; may increase multimer formation... -
Data Freedom: The Expansion of Data Sharing in Research Publications
TypeBlog PostPublishedAug. 5, 2014, 6:51 p.m....reasonable, sensitive to the data type and made as facile for both the author and the reader as possible....