...of interest and co-expressing a cytosolic ZtSH2 reporter, they observe fluorescence localizes to the plasma...And orthogonal sensors with different ITAM/SH2 reporter pairs allow multiplexed measurement of different...10.1101/2022.08.13.503850.  iGECInano NIR calcium reporter Near-infrared (NIR) fluorescent proteins promise...photochemical characteristics than previous NIR calcium reporters iGECI and NIR-GECO2G, and its small size (~1500...

...in the same lane, and the resulting fraction is reported. Figure 2: Normalization with loading controls...equally strong, so the normalized value simply reports which POI band is stronger. The right gel has exactly...abundance on your membrane, which would be directly reported by band intensity. Researchers use later steps...perturbed by the experiment Unfortunately, multiple reports suggest that common housekeeping proteins are influenced...

...integrin antibodies available through Addgene’s repository. The collection includes 26 unique antibodies...in competition with other similar integrins, as reported by IPI and the Springer lab (Hao et al., 2024)...In this study, we, along with the Springer lab, reported the binding specificity, kinetics, and affinity... by IPI. Our integrin antibodies are the first deposit from an expanding collection of readily accessible...

...stage-specific promoter and design a UAS reporter construct, the reporter will only be expressed where and/or...UAS to examine the expression of reporter genes, or to combine reporter expression from one promoter with...

...that uniquely express selectable and/or sortable reporters in certain states of interest. For example, in...above (Sanger sequencing, qPCR, and fluorescent reporter-based) each come with their advantages and disadvantages...the most convenient strategy is the fluorescent reporter based method since it lends itself to cheap, quick...detection with flow cytometry. However, expressing reporters will consume cellular resources and therefore ...

...approaches may become even more powerful following a report that one can use linear repair templates (ie. PCR-derived... 2014). A further development of this approach reported in 2017 uses the direct injection of a CRISPR/...templates (Farboud et al., 2019).   The Mello lab also reported that modifying the 5’ modification of oligos used...mixtures (Doksin et al., 2018). The Mello lab also reported efficient editing using a co-injection marker ...

...sub-cellular localization signals, plant markers, and reporters (Engler et al., 2014). This kit is a great starter...into a multi-TU targeting construct (Cas9, sgRNA, reporter, etc.). This allows for the multiplexing of sgRNAs...really cool MoClo kit, we would love for you to deposit it with us to share it with the scientific community...Multi-Kingdom Golden Gate Cloning Platform.” Scientific Reports, vol. 9, no. 1, Springer Science and Business Media...

...protein analysis products. You can find plasmids deposited by Promega here.    References and resources ...ligands for dopamine D2/D3 receptors. Scientific Reports, 10(1). https://doi.org/10.1038/s41598-020-78827...fluorescence-based ligand-binding assay. Scientific Reports, 6. https://doi.org/10.1038/srep25769 Grimm, J....mitochondrial dynamics in mammalian cells. Biophysics Reports, 3(4–6), 64–72. https://doi.org/10.1007/s41048-...

...LaFleur, who shared information about recent CRISPR deposits from Arlene Sharpe’s lab. X-Chime and Performing...Faulds, who introduced the very first antibody deposit (the Integrin collection) from the Institute for... Thirteen: Guest Contributors of 2023 8 Great Depositor Research Stories Interested in contributing to...

...www.addgene.org/deposit or email deposit@addgene.org for more information or to start a deposit. If you have...

...telling scientists about Addgene’s repository, and explaining how to deposit and request plasmids, but I also...for: The inside scoop from scientists who have deposited plasmids at Addgene.  Learn how discoveries were...

...PubMed to see which articles contain plasmids deposited at Addgene from the abstract page. Check out that...Icon” box next to the “Addgene Nonprofit plasmid repository” choice. This selection should populate your ...see the “Find Plasmids” box on papers that have deposited plasmids. Now that you know how to navigate to...

...viral vectors in the Addgene repository  Name Serotype Category Depositor  Notes pAAV-CAG-emiRFP670...help, with over 100 user-submitted viral vector reports. And don't forget to use the link below to keep...

...possible without the scientists who deposited plasmids to the repository and for the scientific sharing community...the infographic below for stats on our plasmid deposits and requests). Over the past month, we’ve enjoyed...

... Bank (DSHB), directed by David R. Soll, is a repository for monoclonal antibodies, distributing over ...against new cancer biomarkers. We believe that repositories, such as Addgene and the DSHB, play an important... to ensuring affordable access to materials, repositories provide standardized quality-control and technical...

... own the material in our repository. We distribute it on behalf of depositing institutions. Addgene cannot...Help Center is full of information about how to deposit plasmids, store your plasmids or viral items, technical...

...in diverse bacteria Carol Gross’s lab recently deposited “Mobile-CRISPRi” plasmids, a set of modular CRISPRi...If you have a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in...Find CRISPR plasmids by function Find recently deposited plasmids ...

...posts on COVID-19 featuring plasmid tools from depositors who quickly shared their reagents: detection ...expression, and more. We also highlighted reagent repositories and other collaborative efforts in the scientific...detect SARS-CoV-2, including many with plasmids deposited at Addgene! 2. DNA Purification Without a Kit...

...Addgenies, we’re able to feature some recently deposited plasmids every quarter. The “hot plasmids” from...Academic Job Interview by Erik Snapp Addgene depositor and guest blogger Erik Snapp breaks down how to... blog posts Resources at Addgene Browse the repository Find educational resources at Addgene Search ...

...522 organizations in 41 countries enriched our repository with 15,020 new plasmids — a testament to the...viral vector serotypes, or hundreds of Data Hub reports detailing antibody experimental results. We also...options. Almost any AAV transfer plasmid in our repository can now be packaged on demand in five distinct...