We narrowed to 394 results for: HAL

...even axons of up to 100 microns in length. Some challenges of the Brainbow system are that it does not use...Limbal Stem Cell Deficiency." Investigative ophthalmology & visual science 59.3 (2018): 1630-1640. Sakaguchi...

...double strand break repair, it has proven more challenging to develop high efficiency CRISPR-based approaches...delivering CRISPR components as RNPs and delivering two halves of a repair template spread across two AAVs, it...

...experiment, but you’ll only spend half of each day on it. The other half of each day you can spend accomplishing...

...First, the imaging of Brainbow mouse tissue was challenging due to low fluorescence intensity, caused partially...expression patterns. Hampel S, Chung P, McKellar CE, Hall D, Looger LL, Simpson JH. Nature Methods. 2011 Mar...

...transduction of lung cells via airway administration (Halbert et al., 2001). It additionally has tropism for ...-0. https://doi.org/10.1016/j.cell.2025.06.026 Halbert, C. L., Allen, J. M., & Miller, A. D. (2001). Adeno-Associated...

...nn1525 Gunaydin, L. A., Yizhar, O., Berndt, A., Sohal, V. S., Deisseroth, K., & Hegemann, P. (2010). Ultrafast... Augustin, S., Sauter, K., Papagiakoumou, E., Gottschalk, A., Soba, P., Emiliani, V., Engel, A. K., Hegemann...

...important and abundant neurotransmitters, but it is challenging to monitor because only a small number of glutamate...to specific cell types in the brain can be a challenging — but critical — factor for successful experiments...

...Consider talking to people in the lab across the hall or in the same department as well. What do they ... you? Maybe you like things a bit hectic and challenging and this drives your best work. Or is it teamwork...

...Pamela Hines (Senior Editor at Science) Edward J. Hall (Professor of Philosophy at Harvard University) ...experiments they’re reading about weren’t changed halfway through in order to chase a positive result. Beyond...

...Cre-dependent conditional alleles, has remained a challenge. Enter Easi-CRISPR: a method that harnesses the...template. Although effective, ivTRT may be more challenging for sequences with complex secondary structure...

...CRISPR/Cas9, TALENs, and ZFNs). However, several challenges still remain, such as making gene edits completely...nuclease technology with the piggyBac™ system, these challenges can be considerably reduced. The piggyBac™ transposon...

... genomic DNA to amplify the gRNA targets was challenging because you’re limited by the sequence of the...genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nat Methods. 2014 Aug;11(8):783-4. ...

...then flow cytometry can help you overcome these challenges and make these types of experiments easier and...fluorophores Fluorescent antibodies get around the challenges of fluorescent protein fusions by binding to ...

...Creating epitope-tagged clonal cell lines remains a challenge as it relies on inefficient homology-directed ...representing a protein super family). To overcome these challenges, we set out to develop a hassle-free, highly ...

...molecule of GFP. Because red light has approximately half the energy of blue light, two photons of red light...model embryos >15 μm Thsee are some of the most challenging samples to image as they require both optical...

...replicates, spreads, causes neurotoxicity, and is lethal if left untreated (for review see Dietzschold et..., Harris, J.A., Ng, L., Winslow, B., Cain, N., Mihalas, S., Wang, Q., Lau, C., Kuan, L., Henry, A.M., ...

...our pipeline. In it, they break down the unique challenges of our platform and how we were able to address...

...on Twitter Wednesday: 2022 Blue Flame Depositors Hall of Fame  Thursday: 8 Great Depositor Research Stories...

...used tip boxes for a lab supply tree and set a challenge for her lab mates to create the best-recycled ...

... Though 2021 had more than its fair share of challenges, the annual Deck the Lab Contest brought us smiles...