We narrowed to 253 results for: IRES

1. Gendered Innovations: Why Does Sex of the Cell Matter?

   Type

   Blog Post

   Published

   July 21, 2016, 2:30 p.m.

   ...sex, the National Institutes of Health (NIH) now requires that sex be included as a biological variable ...

2. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

   Type

   Blog Post

   Published

   April 22, 2015, 2:06 p.m.

   ...ability to turn on genes at different times. It requires more complex synthetic networks. Addgene: What...

3. 5 Steps to Writing Grant Proposals for Grad Students

   Type

   Blog Post

   Published

   Feb. 11, 2014, 12:13 p.m.

   ...Research at universities typically requires funding from a variety of government and academic institutions...

4. Plasmids 101: Modular Cloning

   Type

   Blog Post

   Published

   March 12, 2024, 1:15 p.m.

   ...simple, and engineering complex cellular behaviors requires making complex manipulations to cells. For example...

5. Viral Vectors 101: Calcium Sensors

   Type

   Blog Post

   Published

   Aug. 10, 2023, 1 p.m.

   ...molecular neuroscience. 2014 Nov 18;7:88. Tian L, Hires SA, Looger LL. Imaging neuronal activity with genetically...

6. Viral Vectors 101: Chemogenetics

   Type

   Blog Post

   Published

   July 11, 2023, 1:15 p.m.

   ...Targeting Receptor Expression Using chemogenetics requires successfully introducing receptors to target cells...

7. Antibodies 101: Choosing the Right Isotype

   Type

   Blog Post

   Published

   April 6, 2023, 3 p.m.

   ...expressed at very low levels or your application requires a high degree of affinity, then species choice...

8. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

   Type

   Blog Post

   Published

   May 7, 2020, 1:15 p.m.

   ...editors The most commonly used S. pyogenes Cas9 requires the target site in the genomic DNA to contain ...

9. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

   Type

   Blog Post

   Published

   May 1, 2018, 1:57 p.m.

   ...of Split-BioID is its speed of labeling. BioID requires 6-24 hours for tetracycline induced expression...

10. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type

    Blog Post

    Published

    Nov. 7, 2017, 1:59 p.m.

    ...genome. AAV belongs to the family Parvoviridae and requires co-infection of helper viruses like adenovirus...

11. Science communication: 9 Strategies to Get Your Foot in the Door

    Type

    Blog Post

    Published

    Sept. 27, 2017, 1:23 p.m.

    ...around climate change, biodiversity, or genomes requires lots of data. Get your community involved in sampling...

12. Engaging with science and society at pgEd

    Type

    Blog Post

    Published

    Sept. 20, 2018, 12:17 p.m.

    ...communication A career in science communication or policy requires broad interest in issues at the interface of science...

13. Hot Plasmids - October 2020

    Type

    Blog Post

    Published

    Oct. 13, 2020, 1:15 p.m.

    ... elegans with CRISPR can be difficult since it requires the much less efficient double stranded templates...

14. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type

    Blog Post

    Published

    Sept. 26, 2019, 12:50 p.m.

    ...with CRISPR components. However, this sometimes requires intracellular injections into the cytoplasm or...

15. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type

    Blog Post

    Published

    July 14, 2016, 2:30 p.m.

    ...Cas9. Cpf1 cleaves DNA in a staggered pattern and requires only one RNA rather than the two (tracrRNA and...

16. Plasmids 101: Terminators and PolyA signals

    Type

    Blog Post

    Published

    March 31, 2016, 2:30 p.m.

    ...certain RNAs. Unlike the eukaryotic mechanism which requires a consensus sequence for the addition of a poly...

17. Tips for Titering Your Lentiviral Preps

    Type

    Blog Post

    Published

    March 15, 2016, 2:30 p.m.

    .... Developing the proper control line typically requires transducing with a limiting multiplicity of infection...

18. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type

    Blog Post

    Published

    Dec. 1, 2015, 3:30 p.m.

    ...proteins). The chemistry is complex and this work requires an interdisciplinary team.  Day two of the conference...

19. Illuminating Epigenetics with A FRET Based Biosensor

    Type

    Blog Post

    Published

    Nov. 19, 2015, 3:30 p.m.

    ... has recently been hitting the headlines, with sotires like the potential devastation of the palm oil ...

20. Gaining Leadership Skills Volunteering at a Professional Organization

    Type

    Blog Post

    Published

    Aug. 6, 2015, 2:30 p.m.

    ...Different leadership styles Being an effective leader requires you to understand how to work with many different...