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We narrowed to 461 results for: MEA;

Showing: 161 - 180 of 461 results

  1. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ...lagged behind, likely due to limitations of current means to detect RNA-protein interactions. To address this... E. coli’s BirA*. Faster labeling kinetics also means that BASU could potentially be used in vivo to study...

  2. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ... are happy to share aliquots of dTAG-13 in the meantime, please address your requests for dTAG molecules...the dTAG lentiviral plasmids can serve as a rapid means of comparing functionality of N- and C-terminal ...

  3. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    Published
    Nov. 30, 2021, 2:15 p.m.
    ...ill-defined. In this article, I try to clarify what people mean when they ask about “problem solving skills” and...because I can resolve issues effectively does not mean I am qualified to be an astronaut. But, knowing ...

  4. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    Published
    Feb. 11, 2014, 12:13 p.m.
    ...single sentence and figure must be clear, concise, meaningful, and supportive of the mission of the application...of ‘to be’ verbs creates strong, well-directed meaning and delivers strength to the sentence. Below are...

  5. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ... genome, the virus is replication incompetent, meaning it can’t replicate and continue the infection cycle... (or BSL-2+) lab and require additional safety measures.   General viral handling safety   Containment...

  7. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ...each part of a split fluorescent protein (FP) and measure the signal produced when the candidate proteins...interaction in vivo or in vitro, and can be used for measuring protein expression or localization in cells, or...

  8. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    Published
    Sept. 26, 2019, 12:50 p.m.
    ...particles (VLPs). VLPs lack viral genomes which means they are non-infectious and non-replicative. Nanoblades...iPSCs and BM cells after Nanoblade treatment as measured by their maintenance of pluripotency marker expression...

  9. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    Published
    July 11, 2019, 1:26 p.m.
    ...strains share a common genetic background, which means that each mouse is genetically identical and all...maximum genetic heterozygosity in the population, meaning they more closely mimic the genetic diversity of...

  10. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...this does not mean that you should apply for everything that’s out there, it just means that you shouldn...

  11. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...FLEx systems are generally considered "Cre-On" meaning that your gene of interest starts in the inverse...Resrouces on the Addgene Blog  Read about Other Means of Genome Engineering Using FLEx AAV Vectors? Learn...

  12. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    Published
    Oct. 22, 2014, 1:57 p.m.
    ...do it well" – even the smallest program. I don't mean have fancy food or a fantastic view (these are nice...Timing is everything Many of my suggestions are meant to keep the event on time. Nobody likes a program...

  13. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...have died, usually after four to five days. This means that viral production takes a little bit longer ...Titers: Where Do They Come From and What Do They Mean? Overcoming the Challenges of Lentivirus Production...

  14. Pooled Library Amplifications

    Type
    Blog Post
    Published
    May 30, 2024, 1:30 p.m.
    ...aim for somewhere between 30x and 200x coverage, meaning that you need 30 to 200 times as many colonies ...recombination, we recommend ensuring the cells used are meant for repetitive sequences (such as STBL4 or Endura...

  15. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    Published
    Sept. 12, 2017, 1:44 p.m.
    ... control assays for AAVs. These steps include measuring viral titer and assessing purity. But Addgene ...get hits to “random” genes. Do these hits always mean the sample is contaminated? No - what really matters...

  17. Transferable Skills: Negotiation

    Type
    Blog Post
    Published
    March 3, 2022, 2 p.m.
    ... sum game - that is, if I get what I want, that means you don’t. In this mindset, one of us wins, the ... negotiation is the most straightforward, it can mean that everyone leaves disappointed (we’ve all won...

  18. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...Specific binding of scFVs to cognate aaRS antigens as measured by suspension bead assay. Image from  Preger et...found a way to use the accumulation of indels to measure time. These plasmids with different promoters driving...

  19. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...limit can feel like a lot of pressure. However, it means that your manager (and you) will be motivated to...time, you’ll know more people in industry, which means you’re more likely to have the right contacts and...

  20. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...suggest retransforming the plasmid into a strain meant for DNA purification. It sounds like extra work,...harvesting and miniprepping at various time points. Measuring the optical density of the culture can indicate...
Showing: 161 - 180 of 461 results