We narrowed to 171 results for: PHI-1;

...target DNA quickly and accurately.  Figure 1: Steps in PCR reaction   This process of denaturation...relies on heat resistant DNA polymerase from the thermophilic bacterium, Thermos aquaticus (Taq). Taq polymerase...

...editing machinery such as CRISPR-Cas. Figure 1: Schematic of targeted gene knock-out (left) and targeted... WM, Curiel DT (2019) Long-term correction of hemophilia B using adenoviral delivery of CRISPR/Cas9. Journal...

... digging deeply into their data.     Fig. 1: Genomic fraction for each of five major ancestry ...have approximately 20% more single nucleotide polymorphisms (SNPs) that map to guides in the Broad’s Genomic...

...genome engineering and microbiology.     References 1. Chen, Sean, et al. "Highly efficient mouse genome...shown that a Cas9 protein from Geobacillus stearothermophilus is more stable in human plasma than the standard...

... reached labs in over 75 countries. Figure 1: SARS-CoV-2 proteins and other components. Image from... from their assay to Addgene.  Drew Endy’s and Philippa Marrack’s labs also deposited plasmids for producing...

...fun together! Laura’s Top 10 Event Planning Tips! 1. Take suggestions Some of Addgene’s best events have...culture At Addgene we organically developed the philosophy of  “work hard, play hard.” Part of the reason...

... done and we’re onto the next story. Figure 1: Addgene’s Agile Approach Sharing is caring There... Sophia K. Cheng...

...testing. You can find Nick on LinkedIn. References 1. Skloot, Rebecca, and Bahni Turpin. The immortal life...whether the cell lines had isoenzymes and genetic polymorphisms specific to their origins. This technique is...

...SpCas9 base editors with editing windows as small as 1-2 nucleotides! To reduce off-target effects associated...Lower on-target activity than BE3; editing window ~1-2 nt  87438  pET42b-HF-BE3  HF-Cas9 (NGG)  Very ...compatible with two-photon microscopy using Ti-Sapphire lasers. pBAD-RDSmCherry1 is a red-shifted variant...

...indel formation and phenotypic changes as early as 1 week post-injection. Livers from these mice were histologically... use of St1Cas9 (~3.3 kb) from Streptococcus thermophilus and a rationally-designed truncated Cas9. Unfortunately...

...halorhodopsins to silence neuronal activity (Figure 1) (Wiegert et al., 2017). How to get an opsin into...2011): 26. PubMed PMID: 21191368. Deubner, Jan, Philippe Coulon, and Ilka Diester. "Optogenetic approaches...