Deep Mutational Scanning with One Pot Saturation Mutagenesis
Type
Blog Post
Published
Feb. 22, 2017, 3:30 p.m.
...Below is a step-by-step overview of the protocol:
Prepare ssDNA template: The wild-type plasmid backbone ...means that if the BbvCI enzyme used for ssDNA preparation nicks the anti-sense strand, then the ssDNA template...