We narrowed to 479 results for: RET
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TypeBlog PostPublishedApril 13, 2023, 1:15 p.m....one type of virus to choose from (lentivirus, retrovirus, AAV, etc.). When you’ve decided on an application...attenuated, nef-deleted HIV-1 strains in vivo. Retrovirology. 2007 Sep 23;4:66. doi: 10.1186/1742-4690-4-...
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How Addgene Creates Content
TypeBlog PostPublishedNov. 17, 2022, 2:15 p.m....quickly." Final design (right). Immediate and immense regret of the graphic designer not shown. However... and knowledge changes and require updating or retiring. CRISPR/Cas9, for example, has gone through rapid... -
Flow Cytometry Readouts: Yes, No, and Everything in Between
TypeBlog PostPublishedAug. 20, 2024, 1:15 p.m....plot. In this post, I’ll walk you through how to interpret the antibody readout on a flow plot. Note that...characterize your samples may allow for better interpretation. Priyamvada Prathima has a research background... -
Improved Plasmid Maps Powered by SnapGene
TypeBlog PostPublishedJune 29, 2017, 1:06 p.m.... and sequence displays are now much easier to interpret and analyze at a glance. For a quick look at just...“Analyze Sequence” button. The maps hosted here retain all the features shown in the static maps discussed... -
The Breast Cancer Microenvironment: A Tumor’s Backstage Team
TypeBlog PostPublishedJan. 31, 2019, 1:32 p.m....growth factor-β signaling, possibly inducing the secretion of growth promoting proteins, such as human growth...microenvironments! Take myoepithelial cells for instance. They retard tumor formation by inhibiting cell growth, invasion... -
RNA Extraction Without A Kit
TypeBlog PostPublishedMarch 24, 2020, 1:15 p.m.... and RNA is retained in the top, aqueous layer, while DNA and other proteins are retained in the interphase... -
Plasmids 101: The protein expression toolbox
TypeBlog PostPublishedFeb. 16, 2023, 7:05 p.m....integrate your choice cDNA. Both lentivirus and retrovirus can package cDNAs for genomic delivery and have...to permanently induce expression? Check out our Retro expression vector and Lenti expression vector. ... -
Summer SciComm Series: Cognitive Load
TypeBlog PostPublishedJune 16, 2022, 1:15 p.m....fiction story that doesn’t require any detailed retention of information or formulation of new knowledge...mental bandwidth. Jargon Learning, using, and retaining new vocabulary takes time and effort (otherwise... -
What's New in CRISPR - November 2021
TypeBlog PostPublishedNov. 18, 2021, 2:15 p.m....for cellular delivery of RNAs By Mike Lacy The retroviral-like protein PEG10 specifically binds its own...demonstrate SEND as a modular system for packaging, secretion, and delivery of specific RNAs, such as Cre mRNA... -
Supporting Rare Disease Research with the 2020 BeHEARD Awards
TypeBlog PostPublishedSept. 8, 2020, 2 p.m....cellular transduction events, we will utilize BRET-, FRET-, and intensity-based signaling biosensors to... -
Seven Tips for Using LinkedIn as a Scientist
TypeBlog PostPublishedOct. 1, 2019, 1:21 p.m....plan for a career outside academia (that would be pretty much all scientists), a LinkedIn profile is absolutely...rules of good resume writing. Include examples of concrete accomplishments in both the technical and “plus... -
Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number
TypeBlog PostPublishedSept. 4, 2018, 12:53 p.m....variable gene expression can make data hard to interpret or can interfere with the precision needed in ...only two fold. Moreover, gene expression levels returned to the baseline after 3 hours with the stabilized... -
Antibodies 101: Fab Fragments
TypeBlog PostPublishedSept. 5, 2024, 1:15 p.m....few commonly available different fragments that retain antigen binding (Figure 2): F(ab')2 - These fragments...fragments are produced by proteolytic digestion that retains an antibody’s disulfide bonds within the hinge ... -
Gendered Innovations: Why Does Sex of the Cell Matter?
TypeBlog PostPublishedJuly 21, 2016, 2:30 p.m....gender analysis and 2) provides case studies with concrete examples of how gender analysis leads to discovery...design. But eyes have been opened—and we cannot return to a world that ignores gender. Many thanks to... -
CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt
TypeBlog PostPublishedApril 22, 2015, 2:06 p.m....Technology has been “working on new experimental and theoretical methods to push the scale of genetic engineering...paper, there actually were not many hurdles. It was pretty straightforward surprisingly. So I think a nice... -
A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells
TypeBlog PostPublishedMay 7, 2020, 1:15 p.m.... domain can edit the short single-stranded DNA stretch that is accessible. Image from Gaudelli et al.,... editors to correct mutations in stem cells and return the corrected cells back to the patients. For example... -
Early Career Researcher Toolbox: Finding Relevant Papers
TypeBlog PostPublishedJan. 9, 2020, 1:45 p.m....access it later. Pro tip Checking the number of retweets, likes, and comments on a tweet about a paper ...papers are connected via citations, in order to return results that are more relevant to your search terms... -
CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9
TypeBlog PostPublishedNov. 14, 2024, 2:15 p.m....particularly effective as it can result in the retention of introns or the removal of exons. Disrupting...microRNA lead to its depletion and Drosha processing retardance. RNA Biol. 2014;11(10):1243-1249. doi:10.1080... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog PostPublishedOct. 8, 2024, 1:15 p.m....resistance genes to help cells survive their own secretions. Pro tip! Historically, colicin-producing plasmids...Resources References Demanèche, S., Bertolla, F., Buret, F., Nalin, R., Sailland, A., Auriol, P., Vogel,... -
Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene
TypeBlog PostPublishedApril 6, 2021, 12:30 p.m....quantification of various intermediate steps, a sizing electrophoretic gel, and qPCR quantification of the final libraries...assembly. Most of the time, these plasmids are returned as one or two partial assemblies. If we obtain...