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We narrowed to 464 results for: SHI

Showing: 161 - 180 of 464 results

  1. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    Published
    April 8, 2025, 1:15 p.m.
    ...multiple sources of error (red) influence these relationships and must be addressed with separate controls...accurately quantified In western blotting, the relationship between protein abundance and band intensity... BioRender.com. To see why this S-shaped relationship poses problems for proteins of very different...

  2. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...and respect your IP rights and data ownership? IP and data ownership should be yours (your institution’...sure you don’t use ELN providers that take any ownership over your data or have direct access to it. Your...

  3. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    Published
    Jan. 4, 2022, 2:15 p.m.
    ...differences, they had a hard time maintaining their friendships. I put “neutral” in quotations here because I...because that can indicate confusion around... Ownership: Misunderstandings about who is responsible for...for ScientistsTransferable Skills Guide: LeadershipTransferable Skills Guide: Teamwork  ...

  4. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ...external video recorder and a mic clipped to her shirt. She rolled several long takes, and we cut it up... videos like this would prove to be difficult. Shipping the gear, and walking everyone through the recording...recorder using a lav mic, clipped to the talent’s shirt. But knowing I would have multiple interview subjects...

  5. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ...non-functional, you may simply introduce a frame-shift mutation. This can be achieved using a restriction...standard ligation reaction resulting in a frame-shifted coding region. It is worth noting that unlike these...specifically designed mutations (not limited to frame shift mutations). Consequently, site directed mutagenesis...

  6. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...GFP to be translated), and second, through a gel shift assay using the PhosTag system (see below). This...phosphate moieties and promotes a robust mobility shift in phosphorylated, but not non-phosphorylated proteins...done using mass spectrometry to identify a mass shift proportional to a single phosphate moiety on the...

  7. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...Karnan, S., Ota, A., Hossain, E., Konishi, Y., Hosokawa, Y., & Konishi, H. (2014). A comparative analysis...j.omtn.2017.03.009 Watakabe, A., Ohtsuka, M., Kinoshita, M., Takaji, M., Isa, K., Mizukami, H., Ozawa,...

  8. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type
    Blog Post
    Published
    Dec. 15, 2016, 3:57 p.m.
    ...recombination genes at low temperatures (30-34˚C).  Shifting bacteria to 42˚C for 15 minutes quickly inactivates... is not required for maintenance. A temperature shift to 42˚C not only allows for activation of the red...Department of Laboratory Medicine at the University of Washington. She is particularly interested in science communication...

  9. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...genetics.120.303564 Kim H, Ishidate T, Ghanta KS, Seth M, Conte D Jr, Shirayama M, Mello CC (2014) A Co-... . https://doi.org/10.1534/genetics.115.178335 Dokshin GA, Ghanta KS, Piscopo KM, Mello CC (2018) Robust...

  10. Participate in the Pets of Addgene Scavenger Hunt

    Type
    Blog Post
    Published
    April 1, 2021, 1:15 p.m.
    ...remotely for the last year to give our lab and shipping teams enough space at HQ and for some of us, that...email from us asking for your mailing address and T shirt size. You have until April 7, 2021 to make your...

  11. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...PMC4269290. Masamizu, Y., Okada, T., Kawasaki, K., Ishibashi, H., Yuasa, S., Takeda, S., Hasegawa, I., and ...PubMed PMID: 1381034. Watakabe, A., Ohtsuka, M., Kinoshita, M., Takaj, M.I., Isa, K., Mizukami, H., Ozawa...

  12. Twenty Years of Sharing Science!

    Type
    Blog Post
    Published
    Jan. 8, 2024, 3:25 p.m.
    ...countries. Just eight days into 2024, we've already shipped 2,078 items to 764 scientists at 375 organizations...different countries. It's safe to say we keep our shipping team busy! But they're not alone: to better serve...

  13. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    Published
    Aug. 27, 2019, 1:08 p.m.
    ... cannot be washed or reused/repurposed in some fashion. Addgenies swapping out printer cartridges can ... striving to set an example of environmental stewardship.  Additional resources on the Addgene blog ...

  14. New Viral Vectors - Spring 2025

    Type
    Blog Post
    Published
    April 29, 2025, 1:30 p.m.
    ...need for their experiments — and we’ve been able to ship them out in record time.  However, our in-stock ... to the convenience of smaller sizes and quick shipping. We continue to expand the collection to meet ...

  15. MassBioEd Career Exploration Day at Addgene

    Type
    Blog Post
    Published
    Nov. 13, 2015, 3:30 p.m.
    ...of busy Addgenies preparing plasmid samples for shipping. This was the favorite part of their visit for...sure to tell us what we already knew about our Shipping Operations Coordinator: “Kory was awesome!”. The...

  16. From our Table to Yours: An Inside Look at Lunch at Addgene

    Type
    Blog Post
    Published
    Nov. 27, 2013, 2:54 p.m.
    ...To-Do list long enough that there is no hope of accomplishing it all. How do we encourage people to take a... Hack Days, and we’ve seen great success at accomplishing “we-should-really-make-time-for-that” type projects...

  17. Celebrating Accomplishments in the Lab

    Type
    Blog Post
    Published
    May 8, 2014, 3:07 p.m.
    ... pizza or pub.” The Cepko Lab celebrates any fellowships, jobs, or paper submissions with dinner and champagne...sharing meals, going on trips, and going to New Hampshire in the summer for the annual lab retreat. How ...

  18. "What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring

    Type
    Blog Post
    Published
    Dec. 17, 2013, 2:47 p.m.
    ...mentors and mentees have fulfilling, productive relationships. I will be sharing what I’ve learned along the...many had examples of very productive mentoring relationships with advisors and others who helped shape their...

  19. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

    Type
    Blog Post
    Published
    June 8, 2023, 1:15 p.m.
    ...release in keeping with their value of shared ownership. Go check it out! References and Resources References... Speranza, E., Chu, C. J., Gola, A., Thakur, N., Shih, R., Yao, L., Yaniv, Z. R., Beuschel, R. T., Kabat...
Showing: 161 - 180 of 464 results