...less risk averse. Similar to the elevated plus maze, the light-dark box is a simple device comprised of...downregulating expression of miR124a achieved a similar improvement in the forced swim test.  Another brain...

...transgenic zebrafish are well established (Felker and Mosimann, 2016). One common and robust strategy uses a ...tail. Compared to the opaque mouse embryo, where similar processes occur over days inside the mother, the... Central PMCID: PMC6247916. Felker, A., and C. Mosimann. "Contemporary zebrafish transgenesis with Tol2...

...tobacco, Agrobacterium infiltration into leaves is a simple and efficient way to overexpress a gene and evaluate...abaxial site (lower surface) of the leaf. This is a simple and easy technique to implement. Success might ... Li, Jian-Feng, et al. "The FAST technique: a simplified Agrobacterium-based transformation method for...

...regions. PHP.eB, an enhanced version of PHP.B, shows similar transduction efficiency, but at a log lower dose...pattern in the mouse brain.  Both labs then took similar routes to verify LY6A’s role as a receptor for ...transduce cells. The Wilson Lab generated results similar to the Deverman Lab when they looked at LY6A protein...

...sgRNAs. Its RNA-guided nature makes CRISPRa much simpler to implement in genome-wide screens over cDNA libraries... must be screened to identify effective ones. Simplified in vivo delivery using SPH mice CRISPRa has largely...PMCID: PMC6412672. Zhou, Haibo, et al. "In vivo simultaneous transcriptional activation of multiple genes...

...identify a large variety of related bacteria with similar Cas systems. Some of these Cas systems have enzymes...of Cas7-11 were reported as up to 85%, broadly similar to shRNA and Cas13 systems in a range of mammalian... matter – the mutated copy could be replaced by simply dragging and dropping in a functional copy of the...

...implemented a policy of never rejecting an idea simply because it's not how things were done in the past...’t have to be perfect or even good. The goal is simply to throw out ideas.  When your five minutes are...If you’re in a time crunch, your break can be as simple as a few minutes of meditation or a walk to the...

...Immobilization is the key to immunoprecipitation and simply refers to the process of anchoring an antibody,...applications Oftentimes, an IP-appropriate antibody simply isn’t available for a specific target. In cases...binding proteins involved in histone modification. Similarly, RNA IP (RIP) can be used to isolate proteins ...

...pBAD-bARGSer-AxeTxe, gas vesicle expression in E. coli is easy: simply grow cells in Lennox LB (10 g/L tryptone, 5 g/L...improved purity and contrast. For in vivo experiments, simply induce injected engineered cells via regular intraperitoneal...a chemical transfection reagent (e.g. PEI-MAX). Simply cotransfect a mixture of 1248 ng of CMV-gvpA-IRES-BFP...

...transfected into human cells. From this result one could simply conclude that the shRNA didn’t work, as the expression...level of Gene X in cells treated with Plasmid A is similar to the expression level of Gene X in the untreated... controls are negative controls that produce a similar product, but do not target an endogenous gene in...

...efficiently trawled their own data? A distinct (perhaps similarly impoverished lab) might apply different or novel...although it might be argued various authors have simply chosen to submit elsewhere). The majority of the...types? And how long should data be archived? Simplifying data access Some of the above mentioned issues...

...between cell types or organisms, promoters must be similarly variable. Bacterial promoters only work in prokaryotic...related species from which they were derived. Similarly, the various eukaryotic cell types (mammalian,...expression mRNA Mammalian expression promoter from the simian vacuolating virus 40 Constitutive  May include ...

...a miniaturized version of Spinach that retains similar levels of fluorescence when bound to DFHBI despite...of artificial guide RNAs that can be used to simultaneously target cellular RNA to specific intracellular... Broccoli are derived from Broccoli, and share similar properties to Broccoli despite binding to a different...

...fastest test that has been developed so far.  A similar method has been optimized to detect the SARS-CoV...and will not give a positive result for highly similar viruses. Unlike Cas12a, Cas13a does not require...illuminator or UV light illuminator. Approach: Highly similar to the SHERLOCK diagnostic test, this test varies...

...edit your gene of choice. These vectors are very similar, both with polylinkers for the addition of gene-specific...at once. Therefore, applications that require simultaneous modification of all alleles of a gene, or which...which require modifying multiple genes simultaneously in the same cell, are more appropriate for a CRISPR-based...

...Magic Pocket Cinema Camera 4K. It’s wonderful and simple to use, but when you need to juggle filming and...perfect height (Fig. 2). We recorded video using a simple, external video recorder and a mic clipped to her...video conferencing software. Interviewees could simply boot up Zoom and record themselves talking to their...

...confrontational situations with others because you simply cannot see things from their point of view and ...herself to the nearby village (and to the reader) by simply listening. Chances are, you know someone like this...how previous iterations of those teams resolved similar issues in the past, or how completely different...

...the immune response. The second strategy is the simple removal of the HIV-1 genome from infected cells...efficiency differed from 48-100% between strains. In a similar experiment, CRISPR reduced the HIV-1 copy number...evidence that an HIV-1 cure may be within our reach. Similar studies have shown that CRISPR can be used to combat...

...postdocs. Unfortunately, even when universities have simple postdoc numbers sorted out there can be gaps in...important take-away from this session was the idea that simply satisfying a percent requirement for diversity ...difficult question but not one that should be abandoned simply because it is difficult and is key to the continued...

...complementary Flpbow construct that is functionally similar to Cre-based Brainbow, but is controlled by FLP...-Veenboer C, Barker N, Klein AM, van Rheenen J, Simons BD, Clevers H. Cell. 2010 Oct 1;143(1):134-44. ...Hampel S, Chung P, McKellar CE, Hall D, Looger LL, Simpson JH. Nature Methods. 2011 Mar;8(3):253-9. doi: 10.1038...