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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 353 results for: Sele

Showing: 161 - 180 of 353 results

  1. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ...includes promoters, UTRs, terminators, etc. Once selected, individual units can be combined into… Transcription...systems as well as additional components such as selectable markers, promoters, enhancers, etc. MoClo is ...

  2. Hot Plasmids: Summer 2025

    Type
    Blog Post
    Published
    Aug. 28, 2025, 1:15 p.m.
    ...lineage, different applications require careful selection or design to ensure reliable readout and avoid...anterograde tracing poses challenges, from non-selective anterograde transport to a lack of genetic control...

  3. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ... Figure 3: ASAP5 for voltage imaging. A) ASAP5 selected for its improved kinetics and responsivity in ...context-dependent responses and molecular patterns of selectivity. With 10 plasmids available as a kit, plus 21...

  4. Pooled Library Amplifications

    Type
    Blog Post
    Published
    May 30, 2024, 1:30 p.m.
    ...Re-transformation of the SPLINTR Libraries Protocol.   Selecting your strain for amplification Since pooled libraries...your transformation, any of these options work! Selecting your media Lastly, you need to decide between ...

  5. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...affinity for a target – and this serotype can be selected to preferentially target your cell type of interest...applications, the ability to direct your voltage sensor selectively is especially essential. If you want to learn...

  6. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...students to do rotations in different labs before selecting an advisor, it is always a good idea to introduce...receiving applications, universities typically invite a select group of students to continue the application process...

  7. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ...programs are optimized differently and you may want to select one (or both) depending on the information you ... click the BLAST button, consider the Program Selection parameter, as this will affect the amount of time...

  8. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ... must be used when selecting which antibodies to use together. It’s best to select primary antibodies ...

  9. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...Find plasmids from this paper at Addgene. East-Seletsky, Alexandra, et al. "Two distinct RNase activities...Find plasmids from this paper at Addgene. East-Seletsky, Alexandra, et al. "RNA Targeting by Functionally...

  10. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    Published
    July 16, 2020, 1:15 p.m.
    ...these observations, we considered the following (noiseless) model for our data: We choose to let yx(t) represent...Harvard University. He is interested in using selection, evolution, and machine learning to solve difficult...

  11. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ... provided by Roger Tsien, who showed that the selective interaction between a bisarsenical dye ligand ...of the genetically encoded tag, the speed and selectivity of the fluorophore attachment, the brightness...

  12. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ...actuators and sensors at Addgene. Cell marking/selection: Expression constructs like plasmids often include...This can serve as an alternative to antibiotic selection. Plasmids of this type may have the GFP under ...

  14. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ... plasmids from this paper at Addgene. 2. East-Seletsky, Alexandra, et al. "Two distinct RNase activities... plasmids from this paper at Addgene. 4. East-Seletsky, Alexandra, et al. "RNA Targeting by Functionally...

  15. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...reasons. Exposure to antibacterial drugs provides a selection pressure, killing off bacteria that are susceptible...stop a treatment early, you may be un-wittingly selecting for less susceptible bacteria that will be more...

  16. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...several platforms for engineering pipelines, we selected Nextflow, a workflow management system for scientific...for rapid testing and refinements, we specified a select small number of input file names or filtered the...

  17. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    .... The research described here is only a small selection of the increasing number of studies using viral...Amine, Vijay Chandrasekar, and Jean-Luc Dreyer. "Selective lentiviral-mediated suppression of microRNA124a...

  19. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ...variables within an experiment, both the design and selection of the positive control plasmids should be highly... Expression Level of Gene X   The design and selection of proper experimental controls is not a trivial...

  20. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...chloramphenicol resistance cassette for positive selection and a p15A origin of replication, making it incompatible...Your strain may require additional antibiotic selection or a special growth media, or if your plasmid ...
Showing: 161 - 180 of 353 results