...work with Grows extremely fast (doubling time of ~ 2 hours) Amenable to genetic manipulation The first...tractable for peroxisomal studies on biogenesis, degradation, and proliferation. Genome manipulation K. phaffii...

...perceived climate change risks. Nature Climate Change, 2(10), 732-735. doi:10.1038/nclimate1547 Kunda, Z. (...approached me for advice on her baby’s health but adamantly refused to get them vaccinated. A classmate told...

...You’ve probably heard that only 2% of our genome is made of protein-coding genes, and you might be wondering...polyadenylation (spA) signal. Figure and caption adapted from Hazan and Bester 2021.    Alternative ...Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity. Science. 2012;337(6096):816...

...integrates into the trnS-leu2 locus.   Figure 2: Introducing Mobile-CRISPRi into bacteria. Top: A ...interference (CRISPRi) has been limited to lab-adapted bacteria because it has been challenging to introduce...for using CRISPRi in many types of bacteria: lab-adapted, environmental isolated, and disease-associated...

...aspects of the work you presented may also be unclear. 2. Make the audience your primary focus When writing...in science communication from across the US and Canada convene at ComSciCon's annual flagship workshop...

...improve the brightness of previous Nano-lanterns by 2-4 fold and permit five-color fluorescent imaging! ...Nano-lantern, showing the technique’s versatility and adaptability to many different contexts. Improving Nano-...

...create a recombinant antibody.     Figure 2: Changes to the heavy chain or light chain genes within...10.1093/ilar.46.3.258  Mould AP, Askari JA, Byron A, Takada Y, Jowitt TA, Humphries MJ (2016) Ligand-induced...

...start selecting and testing systemic capsids! Figure 2 contains a decision tree for selecting a capsid, while... highlighted below the table.     Figure 2: This decision tree can help guide your choice on ...transfer to the adult brain. Nature Biotechnology, 34(2), 204–209. https://doi.org/10.1038/nbt.3440 Goertsen...protocols, visit https://clover.caltech.edu. Figure adapted from Challis et al. 2022. Image created with BioRender.com...New World Monkeys and rodents. Image and caption adapted from Campos et al., 2023. AAV.CAP-Mac is a...doi.org/10.1038/nn.4593 Chen, X., Ravindra Kumar, S., Adams, C. D., Yang, D., Wang, T., Wolfe, D. A., Arokiaraj...Zheng, Q., Beddow, T., Zhao, B., Tobey, I. G., Moncada-Reid, C., Eid, F.-E., Walkey, C. J., Ljungberg,...

...al (Nature. 1988 Jul 28;334(6180):320-5.) PubMed. 2. A segment of the 5' nontranslated region of encephalomyocarditis...disadvantages of the IRES element, scientists have adapted "self-cleaving" 2A peptides into their muliticistronic...blog posts and additional resources into one downloadable PDF that you can save on your desktop. The Plasmids...

...mazF gene and a gene for Zeocin resistance (Figure 2). This MazF-ZeoR cassette is flanked by two direct...While selfish in nature, these systems have been adapted by researchers for many uses when plasmid retention... PMID: 8650219. PubMed Central PMCID: PMC39188. Kamada, Katsuhiko, Fumio Hanaoka, and Stephen K. Burley...

...Hoogsten base pairs and wobble pairs.  Figure 2: (A) The codon wheel depicts the 64 codons that encodes...the late 1980s, scientists even created a codon adaptation index (CAI) that is based on the codon usage ...10.1038/nrg3051 Sharp PM, Li W-H (1987) The codon adaptation index-a measure of directional synonymous codon...

...the necessary steps to perform:      Fig. 2: Quick-stepping to a FASTA file   With our outline...have been developed for a singular purpose (ex. adapter trimming, quality score analysis, k-mer counting...Java, R, etc.) in our pipeline. Crucially, this adaptable property gave us the freedom to use multiple open-source...

... vivo and in vitro applications.   Figure 2: Modular features of DIRECTED particles where the ...trafficked from the “source” to “destination”. Images adapted from Lee and Cheah et al. 2023 bioRxiv (A) and ...:GFP-iPAK4 at 3 or 6 h. Scale bar 5 µm. Images adapted from Lin et al., 2023 bioRxiv, under CC-BY-NC 4.0...

...acceptable variations in DR sequences. Figure 2: comparison of Cas 9 vs. Cas 12a expression cassettes...mitochondrial (mito-mCherry-1xFLAG) localization. Adapted from Liu et al. 2021. Anti-FLAG and anti-HA...

...IgM, IgD, and IgG are deleted.     Figure 2: Isotype switching requires DNA recombination of the... constant domains. This switching helps B cells adapt during the course of an immune response since it...

...cellular responses in addition to antibody response 2) very high immunogenicity 3) and long-lasting immune...which target complementary mRNA sequence for degradation. Viral introduction of shRNA plasmids can induce...

...divided by the off-target activity. Figure 2: Specificity is the measure of off-target activity...years flew by, but since then, scientists have adapted CRISPR nucleases for many more specific research...

...3570-5. doi: 10.1073/pnas.1420294112. Epub 2015 Mar 2. PubMed. Find the plasmids from this publication ...designed for plant expression, PTGs will likely be adaptable to many different biological systems. Although...

...PMID: 20543841. PubMed Central PMCID: PMC2920597. 2. Nathans, Daniel. "Puromycin inhibition of protein...the combined effect of protein synthesis and degradation. These processes control the abundance of proteins...

... would release them into the cytosol. Fig. 2: Endogenous sources of duplexed RNA DNA:RNA hybrids...Transcription, translation, and prone to degradation – those are the words that describe RNA! Double ...