We narrowed to 245 results for: construct

1. Viral Vectors 101: Gamma-Retroviral Packaging Systems

   Type

   Blog Post

   Published

   Aug. 6, 2024, 1:15 p.m.

   ...wildtype LTR promoter is no longer present in these constructs. Why do many γ-retroviral plasmids have similar...

2. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

   Type

   Blog Post

   Published

   March 3, 2020, 2:15 p.m.

   ...multiple DNA fragments. This system has been used to construct plasmids for use in model organisms such as E....

3. Hot Plasmids - May 2022

   Type

   Blog Post

   Published

   May 26, 2022, 1:15 p.m.

   ...sinus vein injection of a CAG-driven mNeonGreen construct in mouse. Image from Goertsen D, et al., 2022....

4. The time and cost required to make a plasmid

   Type

   Blog Post

   Published

   March 23, 2023, 1:15 p.m.

   ...Instead of making one plasmid at a time, try constructing several plasmids in parallel. That way, you ...

5. Plasmids 101: The protein expression toolbox

   Type

   Blog Post

   Published

   Feb. 16, 2023, 7:05 p.m.

   ... promoter or tag prior to introduction of the construct into cells. However, if you would like tunable...

6. Top Requested Lentivirus and AAV of 2016

   Type

   Blog Post

   Published

   Jan. 6, 2017, 3:56 p.m.

   ...lentiviruses). Separating Cas9 and the gRNA into two constructs (i.e., using a dual-vector system as opposed ...

7. R Bodies: Membrane-Rupturing Microscopic Tools

   Type

   Blog Post

   Published

   April 14, 2016, 2:30 p.m.

   ... us to image R bodies inside cells, and these constructs may be useful to future scientists who would ...

8. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

   Type

   Blog Post

   Published

   April 22, 2015, 2:06 p.m.

   ...of building synthetic genomes or genome-scale construction projects. Not from the ground up, but so that...

9. CRISPR/Cas9 FAQs Answered!

   Type

   Blog Post

   Published

   March 13, 2014, 4:08 p.m.

   ...the upper is because the top figure shows the construct from our 'Split RNA' design, where the pre-processing... lower figure with PX330 and PX335, where the construct is from our 'Chimeric RNA' design (meaning the...

10. Addgene: The First Twenty Years

    Type

    Blog Post

    Published

    Dec. 19, 2024, 2:15 p.m.

    ...was rapidly able to support sharing of CRISPR constructs and information across the globe.  “Read the ...

11. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type

    Blog Post

    Published

    Aug. 5, 2021, 12:15 p.m.

    ... depicted here. (A) Schematic diagram of the construction of the dual virus and the experimental approach...

12. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

    Type

    Blog Post

    Published

    May 11, 2021, 1:15 p.m.

    ...Armbruster et al., 2007).  We initially used a construct in which the hM4Di was fused to mCherry. To investigate...

13. GPCRs: How Do They Work and How Do We Study Them?

    Type

    Blog Post

    Published

    Feb. 11, 2021, 2:15 p.m.

    ...signaling pathways mediated by GPCRs? The Kruse Lab constructed plasmids that can be used in peroxidase-catalyzed...

14. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type

    Blog Post

    Published

    July 24, 2018, 12:51 p.m.

    ...This modular and flexible system can be used to construct complex lentiviruses for modification of mammalian...

15. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type

    Blog Post

    Published

    June 26, 2018, 1:33 p.m.

    ...cells. The lab will use plasmids from Addgene to construct NFIX expression vectors as well as reporters of...

16. Choosing the B(right)est Fluorescent Protein: Photostability

    Type

    Blog Post

    Published

    June 8, 2017, 2:30 p.m.

    ...proteins and do not require dedicated plasmids or constructs. It should be noted that the photostability measurements...

17. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type

    Blog Post

    Published

    Nov. 3, 2020, 2:15 p.m.

    ...developed a machine-learning model to help them construct “designer” channelrhodopsins with desired characteristics...

18. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type

    Blog Post

    Published

    Sept. 8, 2020, 2 p.m.

    ...on building upon and depositing their modified constructs to Addgene. Congratulations Terry Hébert! ...

19. Viral Vectors 101: Calcium Sensors

    Type

    Blog Post

    Published

    Aug. 10, 2023, 1 p.m.

    ...organisms. Unlike chemical indicators, these DNA constructs can be delivered to intact tissues and organs...

20. Viral Vectors 101: Virus Safety

    Type

    Blog Post

    Published

    June 22, 2023, 1:15 p.m.

    ...integrated by the virus. If an oncogene overexpression construct is being delivered, that is a higher risk cargo...