We narrowed to 513 results for: gats

...challenging. How do you prepare for this shift and navigate the pitfalls? Here's what you need to know.   ...lead to frustration. Clinging to technical tasksDelegation can feel unnatural for those accustomed to ...Type Indicator or DISC assessments can help you navigate team dynamics effectively and foster a cohesive...

...False positives in original studies and false negatives in replications are unavoidable, but can be minimized...and hypothesis generation, in which data are investigated to look for unexpected trends or differences...possible null is really a true null and not a false negative. Therefore, they will place special emphasis on...

...addition, we worked with many collaborators to aggregate a sequence-confirmed series of clones corresponding...part of our Genetic Pathway Reference Set. These Gateway-compatible entry clones are the product of many...described in this paper. Systematic Functional Interrogation of Rare Cancer Variants Identifies Oncogenic...

...and the subsequent product is then digested and ligated into an appropriate vector. Gibson Assembly, GeneArt...maintained. Recombination efficiency is high enough to negate the need for selection usually required in recombineering... Plasmid Cloning Techniques like SLIC and Golden Gate Cloning Learn How to Verify Your Plasmid Read our...

...pigment precipitation, enhances the blue color of negative colonies, and allows for better differentiation...also lead to a white colony. And also false negatives: These are rare, but if a small fragment is inserted...example is the ccdB gene system commonly used in Gateway® Cloning. There are also methods to select for ...

...introduced the “2in1” vector cloning system - a Gateway compatible approach to simultaneously clone two...Grefen and Blatt, 2012). Similar to traditional Gateway™ cloning, the GOI first needs to be cloned into... the GOIs into the 2in1 vector instead of the GatewayTM cloning BP reaction, where insertion of the GOI...

...geographical regions When we originally started gathering this information, many assumed that those living...location of the institution. Continuing efforts to gather information and expedite change We are continuing...advocating for and with junior scientists, to help them navigate the scientific enterprise and promote their voices...

...The second problem is fundamental. We built a NOR gate because they are used to create any program you ...that took a spaceship to the moon was built on NOR gates. It’s a fundamental unit. We did demonstrate that...that you can use the CRISPR chemistry for a NOR gate. But there are a couple of problems. You couldn’t ...

...significant risk for aerosol contamination. As most centrifugation has to be done outside of a biosafety cabinet...use aerosol-tight caps, compatible with your centrifugation speed, to cover the centrifuge bins. These ... and removed in a biosafety cabinet. For ultracentrifugation, heat sealed tubes can be used for extra ...

...tools in about a week. Taking advantage of Golden Gate cloning, the Haynes lab’s Protocols.io protocol ... enzymes and reader-effector proteins. b) Golden Gate assembly (backbones available at Addgene) with examples...flexibility of the single-pot system will allow investigators to rapidly create multiple synthetic chromatin-modifying...

...complexes, but a serendipitous discovery led them to investigate the use of a common laboratory chemical, guanidine...gene using Cre recombinase and to generate Nfix-negative neural stem cells. The lab will use plasmids from... delay, result in loss of function or dominant negative NFIX phenotypes. Developing a rapid test for ...

... by a variety of methods such as ultracentrifugation, centrifugation on a sucrose gradient, spin columns...high-titer lentivirus preparations without ultracentrifugation. Sci Rep. 2015; 5:13875. PubMed PMID: 26348152...

...work well. It is important that your readers can navigate your poster and follow the logical progression...all cases the idea is the same; the reader will navigate the poster from column to column left-to-right...the Addgene blog Check out this blog post on navigating the poster session Find tips to make the most...

...learned that some of the technologies that helped us navigate a global pandemic can also be the thing that provides...by meeting with professors - a/k/a Principle Investigators (PIs) - whose labs does research that aligns... I hope the tips and tricks above can help you navigate it in confidence.   Ashley Bastin is a first ...

...flow plot. This will make it difficult to properly gate your desired cell population. By compensating between...your experimental samples, you will first need to gate out your main population and singlets. Then, you...More resources on the Addgene blog Introduction to Gating in Flow Cytometry Antibodies 101: Flow Cytometry...

...digest cuts DNA into smaller pieces, and proximity ligation creates chimeric circles of DNA that are distant...into smaller pieces. Sticky ends created are then ligated together to create loops of DNA. This is key to...there was little enrichment of DNA targeted by a negative control gRNA or predicted gRNA off-target sites...

...muscle contractions, and the control of voltage gated ion channels all depend on transmitting and sensing...action potentials which are electrical charges propagated through multiple cells to do things like translate...historically come from a variety of sources – voltage-gated potassium channels found in Drosophila to phosphatases...

...model of the protein. At some point during this interrogation process, they will almost invariably say something...creating physical models of proteins that are being investigated in their labs. Soon after we began working with...gymnastics that this protein must undergo as it interrogates your genome. If you are interested in experiencing...

...solution. Proteins and genomic DNA are removed by centrifugation. At this point, the plasmid DNA sample can ...binding DNA, the glass particles are pelleted by centrifugation and washed before DNA is eluted with water ...phosphoric acid for 3 minutes followed by a short centrifugation for a total of four times before a wash with...

...vitro, before transforming it into E. coli for propagation. However, while not widely known, plasmid assembly...Laboratory of Molecular Biology. He is currently investigating the molecular biology of synaptic transmission...and plasticity.     Javier García-Nafría is an investigator scientist at the MRC Laboratory of Molecular...