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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 406 results for: mag

Showing: 161 - 180 of 406 results

  1. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    Published
    May 12, 2015, 1:32 p.m.
    ...of 1.2 per megabase in mammals), leading to DNA damage and developmental aberrations. In multiple systems...10.1038/ni0707-665. PubMed. Growth inhibition and DNA damage induced by Cre recombinase in mammalian cells. ...

  2. The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

    Type
    Blog Post
    Published
    Nov. 14, 2016, 3:30 p.m.
    ...routinely deposited in Addgene!” As you can imagine, answering plasmid requests can be a time-consuming...researchers from far corners of the world. “It’s easy to imagine how hard it is to distribute thousands of plasmids...

  3. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ...source of mouse-derived single-domain antibodies. Image created in BioRender and adapted from Xu, et al..../fimmu.2017.01589 Jin B, Odongo S, Radwanska M, Magez S (2023) Nanobodies: A Review of Generation, Diagnostics...

  4. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    Published
    March 28, 2022, 4:30 p.m.
    ...experiments directly from Addgene. How Addgene is Reimagining Antibody Sharing Addgene is building our antibody...includes frequently cited antibodies for use in brain imaging (Andrews et al., 2019), validated in both western...

  5. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ... of producing fluorescent proteins and enzymes. Image from Moore et al., 2021.   This TX-TL system... genes known or suspected to be involved in DNA damage response and DNA repair.  A new CRISPR pooled library...

  6. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    Published
    Aug. 2, 2022, 1:15 p.m.
    ...bacteria will be stained red or pink, as shown in the image below. An example of Gram positive (purple...purple) and Gram negative (red/pink) staining. Image credit: Mark Perkins https://www.flickr.com/photos/occbio...

  7. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    Published
    Aug. 4, 2020, 1:15 p.m.
    ... more have been critical for pandemic research. Image from Reproducibility for Everyone. Case study...Comparative tropism, replication kinetics, and cell damage profiling of SARS-CoV-2 and SARS-CoV with implications...

  8. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    Published
    May 16, 2018, 1 p.m.
    ...of the most important steps in the design of an imaging experiment.  The choice is non-trivial, however...Choosing Your Fluorescent Proteins for Multicolor Imaging Resources on Addgene.org Browse all fluorescent...

  9. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...location of the ribosome, TRICK (Translating RNA Imaging by Coat protein Knock-off) instead takes advantage... M., et al. “Translation. An RNA biosensor for imaging the first round of translation from single cells...

  10. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    Published
    Sept. 12, 2019, 1 p.m.
    ...brightest resting fluorescence and can be used for imaging small neuronal processes (dendrites and axons)....maintaining high peak fluorescence) and is useful for imaging signaling activity in large populations of densely-labeled...

  12. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...are sensors available for other ions including magnesium, potassium, and zinc. These ion sensors can be...of them have even been expanded for multiplexed imaging for advanced monitoring of different factors, in...

  13. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ...    Fig. 1: Schematic overview of ChIP. Image courtesy of Shengliu via Wikicommons.   Okay...actually celebrating a successful ChIP experiment! Image courtesy of https://www.wannapik.com/   Even...

  14. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ... hybridomas for monoclonal antibody production. Image created with BioRender.com.   The antibody-producing...different antibodies produced by the hybridoma. Image from Bradbury et al., 2018.   Another barrier...

  15. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    Published
    May 26, 2020, 1:15 p.m.
    ... Figure 1: The SRIRACCHA reporter construct. Image from Wen et al., 2017. Once you’ve introduced...fluorescence.  Figure 2: The GEmCherry reporter. Image from Højland Knudsen et al., 2018. So how does...

  16. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    Published
    June 12, 2018, 11:40 a.m.
    ...chromatin recognition of transcription-associated DNA damage that promotes homologous recombination. Gong F,...small, sensitive immunohistochemical reagents. Yamagata M, Sanes JR Proc Natl Acad Sci U S A. 2018 Feb...

  18. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ... molecules are clearly associated with cellular damage and dysfunction, it is unclear what the precise... that is now vulnerable to various forms of DNA damage, and these structures can in turn lead to genome...

  19. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ... space that would size-exclude an antibody. In imaging applications, smaller affinity reagents means higher...often used for clinical applications, including imaging.  Diabodies are ~60 kDa proteins formed by two ...

  20. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    Published
    Dec. 8, 2016, 3:30 p.m.
    ...succumb to the toxicity of CcdB.   Figure 1: Image from Bernard, P., et al. showing the pKIL 18/19 ...desired insert should not grow.   Figure 2: Image from http://teachline.ls.huji.ac.il/72682/tutorials...
Showing: 161 - 180 of 406 results