We narrowed to 488 results for: mme

...reagents, several journals or organizations now recommend or require authors to use specific PID formats...Addgene_87155)” Please note that we only provide recommendations for how to cite using the PIDs in the material...

...experiment achieving the gRNA representation recommended in the publications associated with these libraries...our viral services. Please send any questions or comments to [email protected]. Finally we’d like to thank...

...promoter results in high R-body expression  (Schrallhammer et al 2012). This made them an appealing target...3759909. PubMed Central PMCID: PMC213417. 2. Schrallhammer, M. et al. Tracing the role of R-bodies in the...

... that future hires will look up to." She also recommends to seek out advice from seasoned PIs who can ...it is once it gets fully up to speed.” He also comments that it is also surprising “ how easily people...

...section. Analyze your plasmid sequence For more immediate, high-level analysis, you can click directly on...us know what you think about the updates in the comments section below and keep checking in at blog.addgene.org...

...plasmid sequencing by NGS. Addgene’s QC scientists immediately screen these plasmids to confirm they contain...samples that are requested by scientists, will immediately be screened by our QC scientists to confirm the...

...These modifications can be done in-lab using commercially available kits or ordered as synthesis modifications... to do-it-yourself with a kit or purchase it commercially synthesized with a label. Not enough options...

...syringe."Feel free to share your alt text in the comments! We learned a lot from listening to others' alt...people.  Whether you start small with alt text or immediately dive into applying these four steps elsewhere...

...with biorender.com. ITR structure  WT ITRs are symmetrical 145-nucleotide sequences that flank the ends ...through ITR sequences. GC tails are often located immediately outside of the ITRs in the plasmid backbone and...

...a variety of stakeholders from industrial and commercial operators, government agencies, active researchers...scientific innovation amongst school children through summer workshops and interactive discussions. Not only...

...guest blogger post about colony PCR, I signed up immediately. I had used colony PCR before and thought it ...your research or career? Share your story in the comments below!   Additional resources on the Addgene...

...engineering is the fact that Cas9 can be easily programmed to make a DNA double strand break (DSB) in the...impact repair is that the Cas9 protein doesn’t immediately release from the broken end after cleavage, which...

...terminus. With that new design in place, Konermann immediately saw an order of magnitude improvement in activation...pooled libraries in your experiments, Addgene recommends that you review the steps for how to use pooled...

...your connections post. Stay in touch by liking, commenting and re-sharing their posts. LinkedIn users notice...turns a stranger into a friendly colleague. Recommended Reading & More: Scientist Networking: What is...

...particularly for applied projects intended for commercialization. Many of the genetic pieces developed over...able to overcome them. Share your stories in the comments below! And if you have developed a genetic tool...

...complementary skill sets, and “talk a good game." Mike recommends that the team should initially include a visionary... an idea or product? Please let us know in the comments section! And read on for more great information...

...their bulk can be a barrier at times. Smaller, slimmer affinity reagents can have deeper penetration into...Single-domain antibodies (sdAbs), sold under the commercial name nanobodies, are derived from a camelid antibody...

...from — Addgene!  Addgene was established as an e-commerce company, which helped it stay ahead of the curve...introductions to lots of molecular biology,” — Reddit commentor Yay4sean, in r/molecularbiology.   Oh, ...

...the both ends and mutated ITR at the center of symmetry. After uncoating in the target cell nucleus, this...use in your experiment! As a starting point, we recommend checking the literature and our AAV data hub to...

...increase capture. Longer histidine tags are also recommended if you need a very pure sample. Because binding...antibody, like this one developed by Dr. James Trimmer’s lab, to perform assays that they otherwise would...