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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 488 results for: mme

Showing: 161 - 180 of 488 results

  3. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ...promoter results in high R-body expression  (Schrallhammer et al 2012). This made them an appealing target...3759909. PubMed Central PMCID: PMC213417. 2. Schrallhammer, M. et al. Tracing the role of R-bodies in the...

  4. Advice for Scientists Starting a Lab

    Type
    Blog Post
    Published
    April 29, 2014, 1:43 p.m.
    ... that future hires will look up to." She also recommends to seek out advice from seasoned PIs who can ...it is once it gets fully up to speed.” He also comments that it is also surprising “ how easily people...

  6. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    Published
    Jan. 28, 2020, 2:35 p.m.
    ...a variety of stakeholders from industrial and commercial operators, government agencies, active researchers...scientific innovation amongst school children through summer workshops and interactive discussions. Not only...

  7. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    Published
    Dec. 11, 2018, 12:46 p.m.
    ...guest blogger post about colony PCR, I signed up immediately. I had used colony PCR before and thought it ...your research or career? Share your story in the comments below!   Additional resources on the Addgene...

  8. Clear and Accessible Writing

    Type
    Blog Post
    Published
    Feb. 15, 2024, 2:15 p.m.
    ...syringe."Feel free to share your alt text in the comments! We learned a lot from listening to others' alt...people.  Whether you start small with alt text or immediately dive into applying these four steps elsewhere...

  9. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    Published
    Oct. 19, 2023, 1:15 p.m.
    ...with biorender.com. ITR structure  WT ITRs are symmetrical 145-nucleotide sequences that flank the ends ...through ITR sequences. GC tails are often located immediately outside of the ITRs in the plasmid backbone and...

  10. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    Published
    June 29, 2017, 1:06 p.m.
    ...section. Analyze your plasmid sequence For more immediate, high-level analysis, you can click directly on...us know what you think about the updates in the comments section below and keep checking in at blog.addgene.org...

  11. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ...engineering is the fact that Cas9 can be easily programmed to make a DNA double strand break (DSB) in the...impact repair is that the Cas9 protein doesn’t immediately release from the broken end after cleavage, which...

  13. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    Published
    Aug. 7, 2014, 1:58 p.m.
    ...your connections post. Stay in touch by liking, commenting and re-sharing their posts. LinkedIn users notice...turns a stranger into a friendly colleague. Recommended Reading & More: Scientist Networking: What is...

  14. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    Published
    July 22, 2014, 5:46 p.m.
    ...particularly for applied projects intended for commercialization. Many of the genetic pieces developed over...able to overcome them. Share your stories in the comments below! And if you have developed a genetic tool...

  16. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...the both ends and mutated ITR at the center of symmetry. After uncoating in the target cell nucleus, this...use in your experiment! As a starting point, we recommend checking the literature and our AAV data hub to...

  17. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...increase capture. Longer histidine tags are also recommended if you need a very pure sample. Because binding...antibody, like this one developed by Dr. James Trimmer’s lab, to perform assays that they otherwise would...

  18. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...from — Addgene!  Addgene was established as an e-commerce company, which helped it stay ahead of the curve...introductions to lots of molecular biology,” — Reddit commentor Yay4sean, in r/molecularbiology.   Oh, ...

  19. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    Published
    Oct. 20, 2022, 1:15 p.m.
    ...These modifications can be done in-lab using commercially available kits or ordered as synthesis modifications... to do-it-yourself with a kit or purchase it commercially synthesized with a label. Not enough options...

  20. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...their bulk can be a barrier at times. Smaller, slimmer affinity reagents can have deeper penetration into...Single-domain antibodies (sdAbs), sold under the commercial name nanobodies, are derived from a camelid antibody...
Showing: 161 - 180 of 488 results