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...brightest teal fluorescent proteins that were photostable and had a high 480/530 nm emission ratio. They...mWasabi is 1.6-fold brighter than EGFP and is photostable. It also has narrow excitation and emission peaks...Directed evolution of a monomeric, bright and photostable version of Clavularia cyan fluorescent protein...

...migration of a cancer cell line equipped with photodissociable EB1 could be stopped in areas with blue light...manipulating and studying cellular dynamics. The photoinducible control of protein function is more specific...contain a smaller RNA tag, are less prone to photobleaching, and achieve better signal in vivo as compared...

...steady stream of papers describing new photosynthetic or photosensory molecules that come from basically ...would be helpful to have molecules with better two-photon properties, so you could activate single cells ...

...range of characteristics (color, brightness, photostability, maturation, oligomerization), many of which...incorporation of biosensors, protein evolution lineages, 2-photon spectra, more elaborate spectrum analysis tools...better way to store and represent within-study photobleaching comparisons.  We very much appreciate feedback...

...concentrating your virus. See our Concentrating AAV Protocol (Step 10) for instructions on how to do this. ...take below but you should read our AAV Production Protocol for additional best practices. Keep your cells...Addgene.org Find Ready-to-Use AAV Preps Find Lab Protocols for Work with Virus Watch Our AAV Purification...

...pseudotyping.   Figure 1: Genome of a prototypical gamma-retrovirus.    Frequently asked questions...mammalian cells using 293T cell-based systems. Curr Protoc Immunol. 2001 May;Chapter 10:Unit 10.17C. doi: ...Research More resources on addgene.org Addgene Protocols: General Transfection The Addgene Retrovirus Guide...

... from it? The image in Figure 1 appears on our protocol page for AAV Purification by Iodixanol Gradient...post.  Here, we are presenting this image on a protocol page on our website, as an instructive step-by-step...chose this alt text: "The left panel is a profile photo of an iodixanol gradient after ultracentrifugation...

...combine computational processing with biological protocols and revolutionary physical approaches. However...the structure in a similar way to the biological protocol, and assembly designs based around the use of ...commercially available tools and components. Rapid prototyping techniques such as 3D printing, on-demand CNC...

...lentiviruses easily made in your own lab. We provide protocols and useful tips for generating your own lentivirus...using this plasmid Cas9 expression is typically cytotoxic, which makes generation of high titer Cas9 lentiviruses...lentiviruses challenging. This cytotoxicity also causes growth rate variability when using lentiCas9-blast...

...In 2017, Lenny Teytelman, CEO of protocols.io, organized a panel to discuss reproducibility issues in...2018 and involved presentations and a handout on protocol sharing, material sharing, computational reproducibility...management plans, electronic lab notebooks, wet lab protocols, reagent sharing, bioinformatic tools, data and...

...selecting a bright fluorescent protein and a photostable fluorescent protein. In the third post of this...applications (e.g. labeling of cells or tracking promotor activity), but it is problematic in applications...verify a number of key properties (brightness, photostability, oligomerization) of a number of promising ...

... area. Unless you are looking for a particular protocol or are trying to replicate an experiment, the ...to the methods section for finer points of the protocols. Are there any red flags about how the work was...Louisiana State University. She is a fan of photosynthesis and writes about autotrophs and biochemistry...

...Resources on addgene.org Western Blot Protocol Western Blot Protocol Video ...Find other tips and tricks for molecular biology protocols Check out other Antibody 101 articles Resources...

... of a BSL-3 lab. Jesse Bloom’s lab published a protocol and reagents for pseudotyping lentivirus with ...methods to use CRISPR to detect SARS-CoV-2. Other protocols and tools for detection have been made openly ... Pettie D, King NP, Balazs AB, Bloom JD (2020) Protocol and Reagents for Pseudotyping Lentiviral Particles...

...CRISPR-Cas activity. For example, fusing AcrA4II to the photo-sensitive LOV2 domain allows anti-CRISPR activity... blue light (Bubeck et al., 2018). 3. Reduce cytotoxicity of CRISPR-Cas editing in tissues The small size... systems prevents the off-target effects and cytotoxicity that result from excessive nuclease activity...

... and delivers it cargo to certain cell types.  Botond Roska’s lab at the Institute of Molecular and Clinical...types (ex: rods or cones) or cell classes (ex: photoreceptors which encompassed both rods and cones).  Targeted...also expressed. If Cre-mCherry expresses in photoreceptors and horizontal cells, and Cre-dependent-GFP...

...behaviors - from basic types like locomotion and motor coordination to more complex situations such as ... using recombinant adeno-associated virus. Curr Protoc Microbiol. (2013); Chapter 14:Unit14D.5. PubMed...Learn about Addgene's viral service Find viral protocols and videos Find AAVs for your research ...

... microbes? Try your hand at photography and participate in a science photo competition. Or nominate yourself...Indonesia. Since then, I have tried many things: photography, making videos, presenting in pubs and at science...

...the Type IIS restriction enzyme and thermocycler protocol. It will also identify overhangs with a high potential...possible, NEB also optimized the thermocycler protocols and made sure that T4 ligase was the correct choice...screening for selection, failed under normal cycling protocols, and required a 48-hour incubation at 37 °C. While...

...viruses also have a propensity to integrate into proto-oncogenes, which can lead to cancer. These viruses...reason for lab accidents is not following safety protocols.) If administering virus to mice, part of your... be safe is to know and closely follow safety protocols. This is especially true when you’re tired, in...