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We narrowed to 507 results for: otos

Showing: 161 - 180 of 507 results

  1. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    Published
    Sept. 14, 2021, 1:15 p.m.
    ...10 kb) or has a low copy number, try following protocols for low copy plasmids. For example, using a longer... intact plasmid. Please see our gel extraction protocol for more details.   Figure 2: An uncut... Find more molecular biology and viral vector protocols Learn about the common lab E. coli strains Find...

  3. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    Published
    May 16, 2018, 1 p.m.
    ...range of characteristics (color, brightness, photostability, maturation, oligomerization), many of which...incorporation of biosensors, protein evolution lineages, 2-photon spectra, more elaborate spectrum analysis tools...better way to store and represent within-study photobleaching comparisons.  We very much appreciate feedback...

  4. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    Published
    March 1, 2018, 2:11 p.m.
    ...concentrating your virus. See our Concentrating AAV Protocol (Step 10) for instructions on how to do this. ...take below but you should read our AAV Production Protocol for additional best practices. Keep your cells...Addgene.org Find Ready-to-Use AAV Preps Find Lab Protocols for Work with Virus Watch Our AAV Purification...

  5. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    Published
    Aug. 6, 2024, 1:15 p.m.
    ...pseudotyping.   Figure 1: Genome of a prototypical gamma-retrovirus.    Frequently asked questions...mammalian cells using 293T cell-based systems. Curr Protoc Immunol. 2001 May;Chapter 10:Unit 10.17C. doi: ...Research More resources on addgene.org Addgene Protocols: General Transfection The Addgene Retrovirus Guide...

  6. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...lentiviruses easily made in your own lab. We provide protocols and useful tips for generating your own lentivirus...using this plasmid Cas9 expression is typically cytotoxic, which makes generation of high titer Cas9 lentiviruses...lentiviruses challenging. This cytotoxicity also causes growth rate variability when using lentiCas9-blast...

  7. Clear and Accessible Writing

    Type
    Blog Post
    Published
    Feb. 15, 2024, 2:15 p.m.
    ... from it? The image in Figure 1 appears on our protocol page for AAV Purification by Iodixanol Gradient...post.  Here, we are presenting this image on a protocol page on our website, as an instructive step-by-step...chose this alt text: "The left panel is a profile photo of an iodixanol gradient after ultracentrifugation...

  8. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    Published
    Feb. 28, 2023, 2:15 p.m.
    ...combine computational processing with biological protocols and revolutionary physical approaches. However...the structure in a similar way to the biological protocol, and assembly designs based around the use of ...commercially available tools and components. Rapid prototyping techniques such as 3D printing, on-demand CNC...

  9. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    Published
    June 15, 2017, 2:30 p.m.
    ...selecting a bright fluorescent protein and a photostable fluorescent protein. In the third post of this...applications (e.g. labeling of cells or tracking promotor activity), but it is problematic in applications...verify a number of key properties (brightness, photostability, oligomerization) of a number of promising ...

  10. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type
    Blog Post
    Published
    Feb. 28, 2019, 1:34 p.m.
    ...In 2017, Lenny Teytelman, CEO of protocols.io, organized a panel to discuss reproducibility issues in...2018 and involved presentations and a handout on protocol sharing, material sharing, computational reproducibility...management plans, electronic lab notebooks, wet lab protocols, reagent sharing, bioinformatic tools, data and...

  11. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type
    Blog Post
    Published
    May 5, 2015, 3:54 p.m.
    ... area. Unless you are looking for a particular protocol or are trying to replicate an experiment, the ...to the methods section for finer points of the protocols. Are there any red flags about how the work was...Louisiana State University. She is a fan of photosynthesis and writes about autotrophs and biochemistry...

  12. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    Published
    Aug. 1, 2024, 1:15 p.m.
    ...Resources on addgene.org Western Blot Protocol Western Blot Protocol Video ...Find other tips and tricks for molecular biology protocols Check out other Antibody 101 articles Resources...

  13. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    Published
    Sept. 27, 2017, 1:23 p.m.
    ... microbes? Try your hand at photography and participate in a science photo competition. Or nominate yourself...Indonesia. Since then, I have tried many things: photography, making videos, presenting in pubs and at science...

  15. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    Published
    Aug. 1, 2023, 1:15 p.m.
    ...the Type IIS restriction enzyme and thermocycler protocol. It will also identify overhangs with a high potential...possible, NEB also optimized the thermocycler protocols and made sure that T4 ligase was the correct choice...screening for selection, failed under normal cycling protocols, and required a 48-hour incubation at 37 °C. While...

  16. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ...viruses also have a propensity to integrate into proto-oncogenes, which can lead to cancer. These viruses...reason for lab accidents is not following safety protocols.) If administering virus to mice, part of your... be safe is to know and closely follow safety protocols. This is especially true when you’re tired, in...

  17. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    ... of a BSL-3 lab. Jesse Bloom’s lab published a protocol and reagents for pseudotyping lentivirus with ...methods to use CRISPR to detect SARS-CoV-2. Other protocols and tools for detection have been made openly ... Pettie D, King NP, Balazs AB, Bloom JD (2020) Protocol and Reagents for Pseudotyping Lentiviral Particles...

  18. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    Published
    July 23, 2020, 1:20 p.m.
    ...CRISPR-Cas activity. For example, fusing AcrA4II to the photo-sensitive LOV2 domain allows anti-CRISPR activity... blue light (Bubeck et al., 2018). 3. Reduce cytotoxicity of CRISPR-Cas editing in tissues The small size... systems prevents the off-target effects and cytotoxicity that result from excessive nuclease activity...

  19. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    Published
    March 12, 2020, 1:15 p.m.
    ... and delivers it cargo to certain cell types.  Botond Roska’s lab at the Institute of Molecular and Clinical...types (ex: rods or cones) or cell classes (ex: photoreceptors which encompassed both rods and cones).  Targeted...also expressed. If Cre-mCherry expresses in photoreceptors and horizontal cells, and Cre-dependent-GFP...

  20. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ...drawbacks of different methods (for comprehensive protocols for all of the methods discussed here refer to...staining. Check Out Our In-Depth Fluoresence Titering Protocol Another popular approach utilizes the antibiotic...pseudotyped HIV-1-based lentiviral vectors."Nature protocols 4.4 (2009): 495-505. PubMed PMID: 19300443. 2....
Showing: 161 - 180 of 507 results