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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 628 results for: rel

Showing: 161 - 180 of 628 results

  1. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ...extract light from a single, thin plane within a relatively thicker three dimensional sample (see Figure ...great image is contrast. The human visual system relies primarily on contrast to identify individual objects...single camera exposure. This is much faster than relying on raster scanning as is done in confocal or 2-... possible. Therefore, 2-photon microscopy which relies on deeply penetrating infra-red excitation light...scanning confocal and 2-photon microscopes which rely on building an entire image pixel by pixel are unable...

  2. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 15, 2017, 1:24 p.m.
    ...between 1,000 nm and 1,200 nm, where there is relatively little tissue absorption, weak tissue scattering...understanding of pathogen-immune cell interaction relied on in vitro studies where it was difficult to apprehend...experiments, experience has shown that some are more reliable and more efficient at tracking cells. Good two-photon...

  3. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ...technique is remarkably straightforward to learn and relatively cheap (compared to many other technologies and...region can result in a particular neurotransmitter release or post-synaptic current. Fortunately, if your ...just optogenetics. Successful stereotaxic surgery relies on identification of the bregma and lambda sutures...coordinates at bregma which allows you to quickly and reliably locate your injection site. We recently purchased...

  4. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    Published
    Nov. 3, 2015, 3:30 p.m.
    ... piñata burst, value was subjectively ascribed relative to the basic needs of each individual; the  community... rational sgRNA design is not possible without relying on some kind of pre-analysis. At the end of 2014... Advanced functions: These functions are not entirely necessary but should be considered extremely useful...microhomology aware”. Trends: Fewer tools are being released with advanced functionality, it seems these kinds... tools dominate the software space but generally rely on less powerful algorithms to detect off-targets...to specify an organism. They do this because they rely on pre built indices or databases in order to find...

  5. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...should the double strand break (DSB) be positioned relative to where the homology ends? A9: The Zhang lab ...

  6. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...overview see Addgene’s AAV guide. AAV particles are relatively small (20 nm) in size which makes them capable...transport: retrograde transport relies on dynein, while anterograde transport relies on kinesin. Different rates...diffusion across the synaptic cleft is usually severely impaired, and only few reports exist about dye...particle is taken up on one side of the cell and released again at another side of the cell after intracellular...visualize neuron connections that are functionally relevant, i.e. activity-dependent neuronal tracing. Apart...

  7. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...non-homologous end joining (NHEJ), HDR occurs at a relatively low frequency, especially in nondividing cells...backbone, sidestepping the problem of HDR vs. NHEJ entirely. The first two classes of base editors were cytosine...

  8. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...signaling dynamics are influenced by the relatively long life-cycle of a protein from expression to degradation...compartment of action via sequestration. The strategy relies on several observations: Rapamycin is a membrane-permeable...physiological setting. Genetic techniques typically rely on overexpression or knock-down, where signaling...or small molecules and exceed a physiologically relevant exposure in intensity and/or duration. Both of...exposure to low-intensity blue light. This approach relies on the incorporation of light-oxygen-voltage (LOV... to this system by engineering cells that lack release factor one (RF-1; Bacterial strain EcAR7) and minimizing...

  9. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...out there (and particularly CRISPR), it is now relatively easy to design constructs and repair templates...the other bits in order to achieve accurate and reliable multi-bit memory. To this end, Chris Voigt and...sites with no cross-talk. These pairs are closely related to the lambda phage integrase system which is the...intracellular pH are required in order to determine the relationships between pH changes and cellular events. To overcome...the oxidation of its substrate, luciferin, which releases energy as a photon. The photon energy is transferred...this interaction and thus triggers a synchronous release of the reporters which can then be tracked along...promoter-selection marker or promoter-inducible expression related vectors are also ready for further chaining. Plasmids...

  10. Plasmids 101: Origin of Replication

    Type
    Blog Post
    Published
    Nov. 11, 2020, 2:23 p.m.
    ...pMB1 (derivative) A Relaxed pBR322 ~15-20 pMB1 A Relaxed pET ~15-20 pBR322 A Relaxed pGEX ~15-20 pBR322... F1** A Relaxed pGEM ~300-500 pUC and F1** A Relaxed pCDF ~20-40 CloDF13 (CDF) D Relaxed This...pBR322 A Relaxed pColE1 ~15-20 ColE1 A Relaxed pR6K ~15-20 R6K* C Stringent pACYC ~10 p15A B Relaxed...replicate the host's chromosomal DNA, but they still rely on the host machinery to make additional copies....Generally, control of replication is referred to as "relaxed" or "stringent" depending on whether the ori is...

  11. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    Published
    Dec. 20, 2017, 2:33 p.m.
    ...Salary –not relevant to most postdocs. Commercial successes in the performing arts- not relevant at all. ...opinions. The contents of this post should not be relied upon for legal advice in any particular circumstance...typographical errors.  No action should be taken in reliance on the information contained in this post and ...paths are not the only place to develop “industry related skills”. Many career paths in academia as well ...look broadly at opportunities to develop skills related to their areas of interest. Additionally, since...institutions of higher education nonprofit entities related to or affiliated with institutions of higher education...applicant works at institutions of higher education, a related or affiliated nonprofit entity, or nonprofit research...

  12. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    Published
    June 24, 2025, 1:15 p.m.
    ...you are ready for us to release your plasmids, so we won’t immediately release preprint-associated materials... depositors had to email us with permission to release their plasmids for distribution, and we’ve found...status — a win-win situation for all. Addgene will release plasmids on hold if they are clearly described ...described in a journal publication. Plasmids will be released 3 years after the deposit date. Don’t worry, we...we’ll let you know before these releases happen (and congratulate you on your paper!). And as always, .... But this way, as soon as we become aware the related journal article has been published we’ll get the...indefinitely. Of course, the fastest way for us to release your plasmids is still for you to notify us as ...

  13. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    Published
    April 19, 2018, 1:25 p.m.
    ...initiative to find a suitable partner, connect the relevant teams, coordinate implementation, and now must...conference calls and make sure to maintain these relationships by keeping in touch by email and social media...media (see the section on “Maintaining Relationships” below). Expand: Collaboration opportunities outside... fun. I just had to remember to approach the relationship with the perspective of my potential collaborators...generally asking for some advice and guidance. The relationships you establish in this way can, of course, lead...favors, but you should not go into a professional relationship expecting these things. In this context, I often...about and get started. You never know where the relationships you develop will lead! Read our blog post about...

  14. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ...plasmid stability, reduced recombination relA or relA1 Relaxed phenotype, mutation eliminating stringent...blue/white screening. F- endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG Φ80dlacZΔM15 Δ(lacZYA-argF)U169... for cloning repetitive DNA. endA1 glnV44 thi-1 relA1 gyrA96 recA1 mcrB+ Δ(lac-proAB) e14- [F' traD36 ...araD139]B/r Δ(codB-lacI)3 galK16 galE15 λ- e14- mcrA0 relA1 rpsL150(strR) spoT1 mcrB1 hsdR2(r-m+)  MG1655  ... ∆lacX74 galK16 galE15 e14-  Φ80dlacZ∆M15 recA1 relA1 endA1 nupG rpsL (StrR) rph spoT1 ∆(mrr-hsdRMS-mcrBC... recombine.  F- endA1 glnV44 thi-1 recA1 gyrA96 relA1 Δ(lac-proAB) mcrA Δ(mcrBC-hsdRMS-mrr) λ-  Stbl3...mcrBC-hsdRMS-mrr) recA1 endA1 gyrA96 gal-thi-1 supE44 λ-relA1 Δ(lac-proAB)/F' proAB+lacIqZΔM15 Tn10 (TetR) TOP10...

  15. How to Be an Excellent Trainee

    Type
    Blog Post
    Published
    Sept. 26, 2023, 1:15 p.m.
    ...planning you can do to build a bench mentor-mentee relationship and bring your best self to the training experience...plenty of articles on building successful mentor relationships, asking for mentor support, and choosing your...the research group). The bench mentor-mentee relationship is extremely important – without a good set ...mentor than your scientific mentor. Thus, this relationship will probably define your experience in the ...Luckily, you can do a lot to contribute to the relationship dynamic and set yourself up for success! Getting.... Preparation before bench work Are there any relevant papers you can read prior to the start of your...through the protocol mentally (or physically where relevant) and identify any gaps or questions you have, ...

  16. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    Published
    March 6, 2014, 7:12 p.m.
    ... mentoring relationship, but if you don’t take this past the chatting stage you will rarely make any change...mentor. How do you make the most of this new relationship? Consider adding formality and active goal setting... setting to your mentoring relationships, so that you can reap rewards in the form of reaching career ...meetings and setting the direction in a mentoring relationship falls on the shoulders of the Mentee. A Mentor...progress over the course of an ongoing mentoring relationship (in a book or online). Mentee: Make a list of...secret of accountability To make a mentoring relationship really productive, the most important step you...SMART goals are Specific, Measurable, Attainable, Relevant, and Time-bound. It is a good idea to take larger...

  17. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ...this, consider the cost of an accidental release. Science relies on popular support, which in turn depends...regions that don't have resident populations of the relevant mosquito species. Reproductive confinement involves... confinement strategies, but they should not be relied on exclusively because historical studies of pathogen...building candidate gene drives intended for eventual release should consider building an appropriate immunizing...mitigate the potential effects of an accidental release3. Which gene drive confinement strategies should... that trust more than an accidental gene drive release, which would represent proof positive that at least...powerful technology responsibly. An accidental release would be particularly dangerous for molecular biology...

  18. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    ...demand for the related plasmid. Under such circumstances, we would observe this same correlation, but it wouldn...pulled the journals from 2005 to 2015 in areas most relevant to plasmids: Genetics & Heredity, Cell Biology...aggregated this much, you can see the 2013 ‘bump’ related to the CRISPR/Cas papers and Addgene deposits, ...Addgene, in contrast to other reasons for the correlation. However, determining this exactly is difficult... remaining difference caused by depositing the related plasmids with Addgene? Probably not all of it. ...still be controlled for. As an example, plasmid-related areas of science could be growing more quickly ...quickly which could also generate this type of correlation. But even with that caveat, it is still suggestive...

  19. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    Published
    Jan. 12, 2021, 2:15 p.m.
    ... build the relationship in a non-awkward way?  7 ways to strengthen your new relationships Here are some...Relationships need nurturing. Perhaps you’ve met someone interesting at an event. Or, perhaps you have...contacts you have; it’s about building genuine relationships with people you care about knowing. How do you...some ways to go about strengthening a new relationship with authenticity and not discomfort: Invite the...meeting (in person or virtually) will make the relationship stronger.  If appropriate, invite them to speak...same people is often the easiest way to build relationships and strengthen them over time.  Things to ...important” or senior people. Focus on building relationships with peers and people around you naturally. ...

  20. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type
    Blog Post
    Published
    May 5, 2015, 3:54 p.m.
    ...familiar with a body of literature related to the topic. Finding reliable references and sorting out what...narrowing down your search and sorting the results (relevance, dates etc). The links for the titles can generally... you an overview of the topic and point you to relevant primary research articles. Then just start reading...should give a critical eye to all results in the relation to how they are supporting the conclusions. Look...key words and download what seems interesting or relevant. Harness the power of the Google search engine...Google Scholar will send you daily updates with relevant literature. Organizing the literature As a scientist... of useful papers. For many, this collection is purely electronic. Gone are the days of file cabinets ...
Showing: 161 - 180 of 628 results