We narrowed to 262 results for: rigi

1. RNA Extraction Without A Kit

   Type

   Blog Post

   Published

   March 24, 2020, 1:15 p.m.

   ...homogenization solution and phenol addition of the original no-kit protocol into one step. After homogenization...

2. Antibodies 101: Affinity Reagents

   Type

   Blog Post

   Published

   April 14, 2022, 1:15 p.m.

   ...the orientation of the two fragments. However, rigidity can be introduced through mutation, allowing diabodies...

3. Visualizing Protein Turnover In Situ

   Type

   Blog Post

   Published

   Jan. 16, 2018, 3:20 p.m.

   ... clever combination of FUNCAT and PLA PLA was originally published by the Söderberg lab in Sweden as a...

4. New Tool for Lineage Tracing: The ClonTracer Library

   Type

   Blog Post

   Published

   Sept. 22, 2015, 2:30 p.m.

   ...drug discovery process.” The ClonTracer library originally came about as Bhang and co-workers were trying...

5. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

   Type

   Blog Post

   Published

   May 5, 2015, 3:54 p.m.

   ...was contributed by Johnna Roose. This post was originally published on Johnna's New Under The Sun Blog ...

6. Working with Nuclear Receptors

   Type

   Blog Post

   Published

   July 15, 2014, 4:03 p.m.

   ... through factors (both of endocrine and dietary origin) that are diffused or actively transported from...

7. A Look at Addgene's QC Process

   Type

   Blog Post

   Published

   May 6, 2025, 1:15 p.m.

   ... something completely different than what was originally intended. Sharing unverified plasmids can lead...

8. Making CRISPR Plasmids Using Fragmid

   Type

   Blog Post

   Published

   Aug. 29, 2024, 1:15 p.m.

   ...contain an ampicillin resistance cassette and an origin of replication, which are not schematized. Sizes...

9. Science communication: 9 Strategies to Get Your Foot in the Door

   Type

   Blog Post

   Published

   Sept. 27, 2017, 1:23 p.m.

   ...check out these two excellent articles: On the Origin of Science Writers How to create a successful science...

10. Fluorescent Proteins 101: Introduction to FRET

    Type

    Blog Post

    Published

    June 27, 2017, 1:03 p.m.

    ... Förster Resonance Energy Transfer (FRET) was originally described by Theodor Förster in 1948 as a variation...

11. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type

    Blog Post

    Published

    July 24, 2018, 12:51 p.m.

    ... higher titer of lentiviral particles than the original lentiCRISPR v1. Special note from the Zhang lab...

12. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type

    Blog Post

    Published

    May 22, 2018, 1:38 p.m.

    ...yeast overexpression vector. This plasmid contains origins of replication and selectable markers that allow...

13. Viral Vectors 101: Chemogenetics

    Type

    Blog Post

    Published

    July 11, 2023, 1:15 p.m.

    ...our chemogenetics poster! This blog post was originally written by Maya Peters Kostman in May 2020 and...

14. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type

    Blog Post

    Published

    Aug. 20, 2019, 12:31 p.m.

    ...health status, diet, cage enrichment, stress level, origin (vendor or in-house mouse breeding colony) Environment...

15. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type

    Blog Post

    Published

    July 11, 2019, 1:26 p.m.

    .../c, and 129. Conversely, outbred strains were originally derived from random crosses between mice of uncharacterized...

16. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type

    Blog Post

    Published

    Dec. 14, 2021, 2:15 p.m.

    ...dCas9. Within a few rounds of cell division, the original methylated strand is diluted to undetectable amounts...

17. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type

    Blog Post

    Published

    Aug. 24, 2021, 1:15 p.m.

    ... the amount of your protein of interest in the original sample. The competitive ELISA is best used for...

18. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type

    Blog Post

    Published

    July 12, 2021, 1:15 p.m.

    ...Originally published Apr 30, 2020 and last updated Jul 12, 2021. What started out as a small collection...

19. Hot Plasmids and Viral Preps - March 2021

    Type

    Blog Post

    Published

    March 23, 2021, 1:15 p.m.

    ...Ward lab created a tool-kit to expand upon these original tools that allows for both spatiotemporal protein...

20. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type

    Blog Post

    Published

    May 7, 2020, 1:15 p.m.

    ...consists of 3 components:  a deaminase (TadA, originally from E.coli, named TadA7.10 in ABE7.10)  a catalytically...