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We narrowed to 512 results for: sam

Showing: 161 - 180 of 512 results

  1. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type
    Blog Post
    Published
    June 22, 2017, 2:30 p.m.
    ...The same way the human body is made up of organs, cells comprise compartments and structures, called ...insights about the proximity of two proteins within the same subcellular structure or protein complex. By coexpressing...dynamic association in live biological fluorescent samples." PloS one 9.4 (2014): e94245. PubMed PMID: 24728133...

  2. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ...well for a neighboring cell type, even within the same brain region or nucleus. Just optimizing AAV expression...vectors, many from people trying to answer the exact same question I was: What AAV vector is best for my specific...wanted to see more negative data (Figure 2.) At the same time, when scientists who support open science, ...

  3. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...instructions in the same producer cells as the virus. Because it is made at the same time as the other ... a retroviral particle, which is essentially the same for viruses and viral vectors. Key to the wild-type...

  4. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...of the drawbacks of kits and typically follow the same principles: cells are lysed, RNAs and proteins are... centrifugation. At this point, the plasmid DNA sample can be treated with RNase to remove RNA contamination... buffers ran out. Homemade buffers performed the same as those provided with the kit. Although not tested...

  5. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...itself A journal club should aim to have many of the same characteristics as a successful meeting: a clear...through the paper figure by figure. Some follow the same figure by figure organization with a different person...stay abreast of current trends in the literature, sampling a breadth of work from a field (perhaps even two...

  6. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    Published
    March 20, 2014, 3:04 p.m.
    ...whatever works for you, but most days go home at the same time. Use time management tools and tactics to plan...need to match up exactly when you fold them. The same with sheets – wash them and put them right back ...family by forming a social circle of people in the same situation. Go to church, join a club, meet the neighbors...

  7. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ...Github and Gitlab, and science-specific tools, e.g. sample management apps, colony management software, and..., not all of them support reproducibility in the same way. When choosing an ELN for your research, be ...commercialization community in greater Boston, while at the same time keeping one foot on the other side of the pond...

  8. How to Be an Excellent Trainee

    Type
    Blog Post
    Published
    Sept. 26, 2023, 1:15 p.m.
    ... a saying that if the sample prep is poor, then the data will be poor. The same is true for training in...you remember where the equipment, reagents, and samples are. Do: Ask your bench mentor if they have time...

  9. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ...instead of human AAG (remember, MPG and AAG are the same protein!) (Chen et al., 2024). They called their...different teams succeeded with this approach around the same time (He et al., 2024; Tong et al., 2024; Ye et ...hypoxanthine from DNA, creating an abasic site. The same protein (or its homologs) has more than one name...

  11. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ...in the software we develop. We would all have the same blind spots, and there wouldn’t be anyone to question...parts of our code, we might be calling something a sample and in other places, we might be calling it an ...love to collaborate, which, incidentally, are the same principles for open science. I hope you enjoyed ...

  12. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    Published
    Sept. 5, 2017, 1:58 p.m.
    ... events were corrupted by unwanted indels on the same allele, probably due to concomitant NHEJ repair....blocking mutation and our intended mutation were on the same oligo, the intended mutation rarely got in. It seemed...mixed with another ssODN template that contains the same CRISPR/Cas-blocking mutation but not the intended...

  13. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type
    Blog Post
    Published
    March 1, 2016, 3:30 p.m.
    ... mix of enzymes is that they can all work at the same temperature, so the entire reaction takes an hour... to complete at 50 °C. After an hour or so, the sample is immediately ready to transform into competent... generate wild type and mutant constructs at the same time, rather than sequentially.   How does Gibson...

  14. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    Published
    Oct. 2, 2018, 12:56 p.m.
    ...talking to people in the lab across the hall or in the same department as well. What do they have to say about...they leave prematurely? Do lab alumni stay in the same field taking projects with them and remaining close...this warning and profoundly regretted it when the same thing happened to her a year later. I hear too many...

  15. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ...NLuc requires 100-fold less protein to produce the same amount of light from an Fluc or RLuc reaction. In...protein that could be co-imaged with GFP using the same wavelength for excitation. The result was the cyan-excitable... K., et al. (5) imaging multiple proteins at the same time in live cells using fluorescent proteins (FPs...

  16. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...chemogenetic receptors delivered by AAV within the same cell types. Not only can we compare these methods...McCarty, D. M., Paul E. Monahan, and Richard J. Samulski. "Self-complementary recombinant adeno-associated...PMC190186. 9. Grieger, Joshua C., and Richard J. Samulski. "Packaging capacity of adeno-associated virus...

  17. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...the power of the technique relies heavily on the same fluorophore being imaged multiple times in order...Additional PA-FP molecules are then activated in the same manner, until enough molecules have been analyzed...18727145. PubMed Central PMCID:PMC4007248. 11.Hess, Samuel T., Thanu PK Girirajan, and Michael D. Mason. "...

  18. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...multiplexed gRNAs? By expressing multiple gRNAs on the same plasmid, you’ll make sure that each cell that gets...situation: you only need to express two gRNAs at the same time. One system you could use is pX333 from the...options that are available from Addgene follow these same assembly principles, but they’re optimized for different...

  19. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    ... these regions, even if they are present in the sample. Other techniques, like electron microscopy and...RNA-bound state (PDB ID 4ZT0, bottom), seen from the same angle. Box highlights the DNA-binding region of ... structure (right, PDB ID 7S4X), viewed from the same angle to highlight conformational differences. HNH...
Showing: 161 - 180 of 512 results